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Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila

Our understanding of the molecular mechanisms underlying sleep homeostasis is limited. We have taken a systematic approach to study neural signaling by the transmitter 5-hydroxytryptamine (5-HT) in drosophila. We have generated knockout and knockin lines for Trh, the 5-HT synthesizing enzyme and all...

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Autores principales: Qian, Yongjun, Cao, Yue, Deng, Bowen, Yang, Guang, Li, Jiayun, Xu, Rui, zhang, Dandan, Huang, Juan, Rao, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648528/
https://www.ncbi.nlm.nih.gov/pubmed/28984573
http://dx.doi.org/10.7554/eLife.26519
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author Qian, Yongjun
Cao, Yue
Deng, Bowen
Yang, Guang
Li, Jiayun
Xu, Rui
zhang, Dandan
Huang, Juan
Rao, Yi
author_facet Qian, Yongjun
Cao, Yue
Deng, Bowen
Yang, Guang
Li, Jiayun
Xu, Rui
zhang, Dandan
Huang, Juan
Rao, Yi
author_sort Qian, Yongjun
collection PubMed
description Our understanding of the molecular mechanisms underlying sleep homeostasis is limited. We have taken a systematic approach to study neural signaling by the transmitter 5-hydroxytryptamine (5-HT) in drosophila. We have generated knockout and knockin lines for Trh, the 5-HT synthesizing enzyme and all five 5-HT receptors, making it possible for us to determine their expression patterns and to investigate their functional roles. Loss of the Trh, 5HT1a or 5HT2b gene decreased sleep time whereas loss of the Trh or 5HT2b gene diminished sleep rebound after sleep deprivation. 5HT2b expression in a small subset of, probably a single pair of, neurons in the dorsal fan-shaped body (dFB) is functionally essential: elimination of the 5HT2b gene from these neurons led to loss of sleep homeostasis. Genetic ablation of 5HT2b neurons in the dFB decreased sleep and impaired sleep homeostasis. Our results have shown that serotonergic signaling in specific neurons is required for the regulation of sleep homeostasis.
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spelling pubmed-56485282017-10-23 Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila Qian, Yongjun Cao, Yue Deng, Bowen Yang, Guang Li, Jiayun Xu, Rui zhang, Dandan Huang, Juan Rao, Yi eLife Neuroscience Our understanding of the molecular mechanisms underlying sleep homeostasis is limited. We have taken a systematic approach to study neural signaling by the transmitter 5-hydroxytryptamine (5-HT) in drosophila. We have generated knockout and knockin lines for Trh, the 5-HT synthesizing enzyme and all five 5-HT receptors, making it possible for us to determine their expression patterns and to investigate their functional roles. Loss of the Trh, 5HT1a or 5HT2b gene decreased sleep time whereas loss of the Trh or 5HT2b gene diminished sleep rebound after sleep deprivation. 5HT2b expression in a small subset of, probably a single pair of, neurons in the dorsal fan-shaped body (dFB) is functionally essential: elimination of the 5HT2b gene from these neurons led to loss of sleep homeostasis. Genetic ablation of 5HT2b neurons in the dFB decreased sleep and impaired sleep homeostasis. Our results have shown that serotonergic signaling in specific neurons is required for the regulation of sleep homeostasis. eLife Sciences Publications, Ltd 2017-10-06 /pmc/articles/PMC5648528/ /pubmed/28984573 http://dx.doi.org/10.7554/eLife.26519 Text en © 2017, Qian et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Qian, Yongjun
Cao, Yue
Deng, Bowen
Yang, Guang
Li, Jiayun
Xu, Rui
zhang, Dandan
Huang, Juan
Rao, Yi
Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
title Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
title_full Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
title_fullStr Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
title_full_unstemmed Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
title_short Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
title_sort sleep homeostasis regulated by 5ht2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648528/
https://www.ncbi.nlm.nih.gov/pubmed/28984573
http://dx.doi.org/10.7554/eLife.26519
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