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Phylogeographic and phylogenetic analysis for Tripterygium species delimitation

Tripterygium wilfordii (Celastraceae) is a traditional Chinese medicine; and the dried root and rhizome constitute the main officinal parts. Tripterygium wilfordii has been identified as a potential candidate for the treatment of systemic lupus erythematosus, rheumatoid arthritis, nephritis, asthma,...

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Autores principales: Ma, Baowei, Hu, Tianyuan, Li, Pei, Yuan, Qingjun, Lin, Zhaoshou, Tu, Yuhe, Li, Jia, Zhang, Xianan, Wu, Xiaoyi, Wang, Xiujuan, Huang, Luqi, Gao, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648662/
https://www.ncbi.nlm.nih.gov/pubmed/29075476
http://dx.doi.org/10.1002/ece3.3344
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author Ma, Baowei
Hu, Tianyuan
Li, Pei
Yuan, Qingjun
Lin, Zhaoshou
Tu, Yuhe
Li, Jia
Zhang, Xianan
Wu, Xiaoyi
Wang, Xiujuan
Huang, Luqi
Gao, Wei
author_facet Ma, Baowei
Hu, Tianyuan
Li, Pei
Yuan, Qingjun
Lin, Zhaoshou
Tu, Yuhe
Li, Jia
Zhang, Xianan
Wu, Xiaoyi
Wang, Xiujuan
Huang, Luqi
Gao, Wei
author_sort Ma, Baowei
collection PubMed
description Tripterygium wilfordii (Celastraceae) is a traditional Chinese medicine; and the dried root and rhizome constitute the main officinal parts. Tripterygium wilfordii has been identified as a potential candidate for the treatment of systemic lupus erythematosus, rheumatoid arthritis, nephritis, asthma, leprosy, and cancer. The phylogenetic relationships within the Tripterygium genus are ambiguous; thus, our aim is to clarify the relationships within this genus using phylogeographic and phylogenetic analyses. Here, we first sequenced three plastid DNA regions (i.e., psbA‐trnH, rpl32‐trnL, and trnL‐trnF) and found that Tripterygium hypoglaucum and T. wilfordii were clustered together based on the strength of the topology in the phylogenetic analysis: T. hypoglaucum is polyphyletic, and T. wilfordii is paraphyletic. A spatial analysis of molecular variance showed that the best group value is 4, and the groups were almost consistent with the topology of in the phylogenetic analysis. The Mantel analyses of Tripterygium using IBD web showed statistically significant relationships between genetic and geographical distance distributions (r = .3479, p < .0001). The molecular dating using Fossil calibration indicated that the divergence in Tripterygium was approximately 8.13 Ma. Furthermore, we also analyzed four DNA regions (i.e., ITS2, psbA‐trnH, matK, and rbcL) that were obtained from the NCBI nucleotide database; these results showed that T. wilfordii and T. hypoglaucum clustered together, while Tripterygium regelii represented a separate cluster. Tripterygium hypoglaucum and T. wilfordii were never distinct lineages, and the species circumscriptions are artificial. We propose that T. wilfordii and T. hypoglaucum are conspecific, while T. regelii likely constitutes a separate species.
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spelling pubmed-56486622017-10-26 Phylogeographic and phylogenetic analysis for Tripterygium species delimitation Ma, Baowei Hu, Tianyuan Li, Pei Yuan, Qingjun Lin, Zhaoshou Tu, Yuhe Li, Jia Zhang, Xianan Wu, Xiaoyi Wang, Xiujuan Huang, Luqi Gao, Wei Ecol Evol Original Research Tripterygium wilfordii (Celastraceae) is a traditional Chinese medicine; and the dried root and rhizome constitute the main officinal parts. Tripterygium wilfordii has been identified as a potential candidate for the treatment of systemic lupus erythematosus, rheumatoid arthritis, nephritis, asthma, leprosy, and cancer. The phylogenetic relationships within the Tripterygium genus are ambiguous; thus, our aim is to clarify the relationships within this genus using phylogeographic and phylogenetic analyses. Here, we first sequenced three plastid DNA regions (i.e., psbA‐trnH, rpl32‐trnL, and trnL‐trnF) and found that Tripterygium hypoglaucum and T. wilfordii were clustered together based on the strength of the topology in the phylogenetic analysis: T. hypoglaucum is polyphyletic, and T. wilfordii is paraphyletic. A spatial analysis of molecular variance showed that the best group value is 4, and the groups were almost consistent with the topology of in the phylogenetic analysis. The Mantel analyses of Tripterygium using IBD web showed statistically significant relationships between genetic and geographical distance distributions (r = .3479, p < .0001). The molecular dating using Fossil calibration indicated that the divergence in Tripterygium was approximately 8.13 Ma. Furthermore, we also analyzed four DNA regions (i.e., ITS2, psbA‐trnH, matK, and rbcL) that were obtained from the NCBI nucleotide database; these results showed that T. wilfordii and T. hypoglaucum clustered together, while Tripterygium regelii represented a separate cluster. Tripterygium hypoglaucum and T. wilfordii were never distinct lineages, and the species circumscriptions are artificial. We propose that T. wilfordii and T. hypoglaucum are conspecific, while T. regelii likely constitutes a separate species. John Wiley and Sons Inc. 2017-09-22 /pmc/articles/PMC5648662/ /pubmed/29075476 http://dx.doi.org/10.1002/ece3.3344 Text en © 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Ma, Baowei
Hu, Tianyuan
Li, Pei
Yuan, Qingjun
Lin, Zhaoshou
Tu, Yuhe
Li, Jia
Zhang, Xianan
Wu, Xiaoyi
Wang, Xiujuan
Huang, Luqi
Gao, Wei
Phylogeographic and phylogenetic analysis for Tripterygium species delimitation
title Phylogeographic and phylogenetic analysis for Tripterygium species delimitation
title_full Phylogeographic and phylogenetic analysis for Tripterygium species delimitation
title_fullStr Phylogeographic and phylogenetic analysis for Tripterygium species delimitation
title_full_unstemmed Phylogeographic and phylogenetic analysis for Tripterygium species delimitation
title_short Phylogeographic and phylogenetic analysis for Tripterygium species delimitation
title_sort phylogeographic and phylogenetic analysis for tripterygium species delimitation
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648662/
https://www.ncbi.nlm.nih.gov/pubmed/29075476
http://dx.doi.org/10.1002/ece3.3344
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