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Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance
Antimicrobial resistance is a significant threat to the treatment of infectious disease. Multiple mechanisms of resistance to different classes of antibiotics have been identified and well-studied. However, these mechanisms are studied with bacteria in isolation, whereas often, infections have a pol...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648753/ https://www.ncbi.nlm.nih.gov/pubmed/29062489 http://dx.doi.org/10.1038/s41522-017-0035-0 |
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author | Beaudoin, T. Yau, Y. C. W. Stapleton, P. J. Gong, Y. Wang, P. W. Guttman, D. S. Waters, V. |
author_facet | Beaudoin, T. Yau, Y. C. W. Stapleton, P. J. Gong, Y. Wang, P. W. Guttman, D. S. Waters, V. |
author_sort | Beaudoin, T. |
collection | PubMed |
description | Antimicrobial resistance is a significant threat to the treatment of infectious disease. Multiple mechanisms of resistance to different classes of antibiotics have been identified and well-studied. However, these mechanisms are studied with bacteria in isolation, whereas often, infections have a polymicrobial basis. Using a biofilm slide chamber model, we visualized the formation and development of clinical Pseudomonas aeruginosa biofilms in the presence of secreted Staphylococcus aureus exoproducts, two bacteria that commonly co-infect pediatric patients with cystic fibrosis. We showed that, over time, certain isolates of P. aeruginosa can form different biofilm architecture in the presence of S. aureus exoproducts. We further determined that this interaction was dependent on Psl produced by P. aeruginosa and staphylococcal protein A from S. aureus. Importantly, we identified a mechanism of antibiotic resistance to tobramycin that is dependent on the polymicrobial interactions between these two bacteria. This interaction occurred in isolates of P. aeruginosa recovered from children with cystic fibrosis who failed to clear P. aeruginosa following inhaled tobramycin treatment. |
format | Online Article Text |
id | pubmed-5648753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56487532017-10-23 Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance Beaudoin, T. Yau, Y. C. W. Stapleton, P. J. Gong, Y. Wang, P. W. Guttman, D. S. Waters, V. NPJ Biofilms Microbiomes Article Antimicrobial resistance is a significant threat to the treatment of infectious disease. Multiple mechanisms of resistance to different classes of antibiotics have been identified and well-studied. However, these mechanisms are studied with bacteria in isolation, whereas often, infections have a polymicrobial basis. Using a biofilm slide chamber model, we visualized the formation and development of clinical Pseudomonas aeruginosa biofilms in the presence of secreted Staphylococcus aureus exoproducts, two bacteria that commonly co-infect pediatric patients with cystic fibrosis. We showed that, over time, certain isolates of P. aeruginosa can form different biofilm architecture in the presence of S. aureus exoproducts. We further determined that this interaction was dependent on Psl produced by P. aeruginosa and staphylococcal protein A from S. aureus. Importantly, we identified a mechanism of antibiotic resistance to tobramycin that is dependent on the polymicrobial interactions between these two bacteria. This interaction occurred in isolates of P. aeruginosa recovered from children with cystic fibrosis who failed to clear P. aeruginosa following inhaled tobramycin treatment. Nature Publishing Group UK 2017-10-19 /pmc/articles/PMC5648753/ /pubmed/29062489 http://dx.doi.org/10.1038/s41522-017-0035-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Beaudoin, T. Yau, Y. C. W. Stapleton, P. J. Gong, Y. Wang, P. W. Guttman, D. S. Waters, V. Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance |
title | Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance |
title_full | Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance |
title_fullStr | Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance |
title_full_unstemmed | Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance |
title_short | Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance |
title_sort | staphylococcus aureus interaction with pseudomonas aeruginosa biofilm enhances tobramycin resistance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648753/ https://www.ncbi.nlm.nih.gov/pubmed/29062489 http://dx.doi.org/10.1038/s41522-017-0035-0 |
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