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Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects

Spontaneous bone regeneration could occur to reestablish mandibular bony continuity in patients who underwent partial or total mandibulectomy for tumors with periosteum-preserving. However, scarce data is available related to the precise role of periosteum in this bone regeneration. Therefore we aim...

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Autores principales: Li, Zheyi, Pan, Juli, Ma, Jinling, Zhang, Zhen, Bai, Yuxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648814/
https://www.ncbi.nlm.nih.gov/pubmed/29051537
http://dx.doi.org/10.1038/s41598-017-13586-8
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author Li, Zheyi
Pan, Juli
Ma, Jinling
Zhang, Zhen
Bai, Yuxing
author_facet Li, Zheyi
Pan, Juli
Ma, Jinling
Zhang, Zhen
Bai, Yuxing
author_sort Li, Zheyi
collection PubMed
description Spontaneous bone regeneration could occur to reestablish mandibular bony continuity in patients who underwent partial or total mandibulectomy for tumors with periosteum-preserving. However, scarce data is available related to the precise role of periosteum in this bone regeneration. Therefore we aimed to investigate the gene expression of periosteum that were involved in the mandibular bone regeneration. Mandibular segmental defects were created in six mini-pigs with periosteum preserved. The periosteum of defects and control site were harvested at 1 and 2 weeks. Gene ontology (GO) analysis showed that the mechanisms concerning immature wound healing were clearly up-regulated at week 1. In contrast, by week-2, the GO categories of skeletal development, ossification and bone mineralization were significantly over-represented at week-2 with several genes encoding cell differentiation, extracellular matrix formation, and anatomical structure development. Furthermore, Tgfβ/Bmp, Wnt and Notch signaling were all related to the osteogenic process in this study. Besides osteogenesis, genes related to angiogenesis and neurogenesis were also prominent at week-2. These findings revealed that the gene expression profile of the periosteum’s cells participating in bone regeneration varied in different time points, and numbers of candidate genes that differentially expressed during early healing stages of intramembranous bone regeneration were suggested.
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spelling pubmed-56488142017-10-26 Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects Li, Zheyi Pan, Juli Ma, Jinling Zhang, Zhen Bai, Yuxing Sci Rep Article Spontaneous bone regeneration could occur to reestablish mandibular bony continuity in patients who underwent partial or total mandibulectomy for tumors with periosteum-preserving. However, scarce data is available related to the precise role of periosteum in this bone regeneration. Therefore we aimed to investigate the gene expression of periosteum that were involved in the mandibular bone regeneration. Mandibular segmental defects were created in six mini-pigs with periosteum preserved. The periosteum of defects and control site were harvested at 1 and 2 weeks. Gene ontology (GO) analysis showed that the mechanisms concerning immature wound healing were clearly up-regulated at week 1. In contrast, by week-2, the GO categories of skeletal development, ossification and bone mineralization were significantly over-represented at week-2 with several genes encoding cell differentiation, extracellular matrix formation, and anatomical structure development. Furthermore, Tgfβ/Bmp, Wnt and Notch signaling were all related to the osteogenic process in this study. Besides osteogenesis, genes related to angiogenesis and neurogenesis were also prominent at week-2. These findings revealed that the gene expression profile of the periosteum’s cells participating in bone regeneration varied in different time points, and numbers of candidate genes that differentially expressed during early healing stages of intramembranous bone regeneration were suggested. Nature Publishing Group UK 2017-10-19 /pmc/articles/PMC5648814/ /pubmed/29051537 http://dx.doi.org/10.1038/s41598-017-13586-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Zheyi
Pan, Juli
Ma, Jinling
Zhang, Zhen
Bai, Yuxing
Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects
title Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects
title_full Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects
title_fullStr Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects
title_full_unstemmed Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects
title_short Microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects
title_sort microarray gene expression of periosteum in spontaneous bone regeneration of mandibular segmental defects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648814/
https://www.ncbi.nlm.nih.gov/pubmed/29051537
http://dx.doi.org/10.1038/s41598-017-13586-8
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