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Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation

Ultraviolet radiation (UVR) from sunlight is the major effector for skin aging and carcinogenesis. However, genes and pathways altered by solar-simulated UVR (ssUVR), a mixture of UVA and UVB, are not well characterized. Here we report global changes in gene expression as well as associated pathways...

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Autores principales: Marais, Thomas L. Des, Kluz, Thomas, Xu, Dazhong, Zhang, Xiaoru, Gesumaria, Lisa, Matsui, Mary S., Costa, Max, Sun, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648893/
https://www.ncbi.nlm.nih.gov/pubmed/29051608
http://dx.doi.org/10.1038/s41598-017-13765-7
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author Marais, Thomas L. Des
Kluz, Thomas
Xu, Dazhong
Zhang, Xiaoru
Gesumaria, Lisa
Matsui, Mary S.
Costa, Max
Sun, Hong
author_facet Marais, Thomas L. Des
Kluz, Thomas
Xu, Dazhong
Zhang, Xiaoru
Gesumaria, Lisa
Matsui, Mary S.
Costa, Max
Sun, Hong
author_sort Marais, Thomas L. Des
collection PubMed
description Ultraviolet radiation (UVR) from sunlight is the major effector for skin aging and carcinogenesis. However, genes and pathways altered by solar-simulated UVR (ssUVR), a mixture of UVA and UVB, are not well characterized. Here we report global changes in gene expression as well as associated pathways and upstream transcription factors in human keratinocytes exposed to ssUVR. Human HaCaT keratinocytes were exposed to either a single dose or 5 repetitive doses of ssUVR. Comprehensive analyses of gene expression profiles as well as functional annotation were performed at 24 hours post irradiation. Our results revealed that ssUVR modulated genes with diverse cellular functions changed in a dose-dependent manner. Gene expression in cells exposed to a single dose of ssUVR differed significantly from those that underwent repetitive exposures. While single ssUVR caused a significant inhibition in genes involved in cell cycle progression, especially G2/M checkpoint and mitotic regulation, repetitive ssUVR led to extensive changes in genes related to cell signaling and metabolism. We have also identified a panel of ssUVR target genes that exhibited persistent changes in gene expression even at 1 week after irradiation. These results revealed a complex network of transcriptional regulators and pathways that orchestrate the cellular response to ssUVR.
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spelling pubmed-56488932017-10-26 Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation Marais, Thomas L. Des Kluz, Thomas Xu, Dazhong Zhang, Xiaoru Gesumaria, Lisa Matsui, Mary S. Costa, Max Sun, Hong Sci Rep Article Ultraviolet radiation (UVR) from sunlight is the major effector for skin aging and carcinogenesis. However, genes and pathways altered by solar-simulated UVR (ssUVR), a mixture of UVA and UVB, are not well characterized. Here we report global changes in gene expression as well as associated pathways and upstream transcription factors in human keratinocytes exposed to ssUVR. Human HaCaT keratinocytes were exposed to either a single dose or 5 repetitive doses of ssUVR. Comprehensive analyses of gene expression profiles as well as functional annotation were performed at 24 hours post irradiation. Our results revealed that ssUVR modulated genes with diverse cellular functions changed in a dose-dependent manner. Gene expression in cells exposed to a single dose of ssUVR differed significantly from those that underwent repetitive exposures. While single ssUVR caused a significant inhibition in genes involved in cell cycle progression, especially G2/M checkpoint and mitotic regulation, repetitive ssUVR led to extensive changes in genes related to cell signaling and metabolism. We have also identified a panel of ssUVR target genes that exhibited persistent changes in gene expression even at 1 week after irradiation. These results revealed a complex network of transcriptional regulators and pathways that orchestrate the cellular response to ssUVR. Nature Publishing Group UK 2017-10-19 /pmc/articles/PMC5648893/ /pubmed/29051608 http://dx.doi.org/10.1038/s41598-017-13765-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Marais, Thomas L. Des
Kluz, Thomas
Xu, Dazhong
Zhang, Xiaoru
Gesumaria, Lisa
Matsui, Mary S.
Costa, Max
Sun, Hong
Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation
title Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation
title_full Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation
title_fullStr Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation
title_full_unstemmed Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation
title_short Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation
title_sort transcription factors and stress response gene alterations in human keratinocytes following solar simulated ultra violet radiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648893/
https://www.ncbi.nlm.nih.gov/pubmed/29051608
http://dx.doi.org/10.1038/s41598-017-13765-7
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