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Constitutive expression of OsDof4, encoding a C(2)-C(2) zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.)

BACKGROUND: Dof (DNA binding with one finger) proteins, a class of plant-specific transcription factors which contain a conserved C2-C2-type zinc finger domain, are involved in many fundamental processes. In the Arabidopsis photoperiod response pathway, CDF (CYCLING DOF FACTOR) proteins have a prima...

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Autores principales: Wu, Qi, Liu, Xue, Yin, Dedong, Yuan, Hua, Xie, Qi, Zhao, Xianfeng, Li, Xiaobing, Zhu, Lihuang, Li, Shigui, Li, Dayong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649077/
https://www.ncbi.nlm.nih.gov/pubmed/29052517
http://dx.doi.org/10.1186/s12870-017-1109-0
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author Wu, Qi
Liu, Xue
Yin, Dedong
Yuan, Hua
Xie, Qi
Zhao, Xianfeng
Li, Xiaobing
Zhu, Lihuang
Li, Shigui
Li, Dayong
author_facet Wu, Qi
Liu, Xue
Yin, Dedong
Yuan, Hua
Xie, Qi
Zhao, Xianfeng
Li, Xiaobing
Zhu, Lihuang
Li, Shigui
Li, Dayong
author_sort Wu, Qi
collection PubMed
description BACKGROUND: Dof (DNA binding with one finger) proteins, a class of plant-specific transcription factors which contain a conserved C2-C2-type zinc finger domain, are involved in many fundamental processes. In the Arabidopsis photoperiod response pathway, CDF (CYCLING DOF FACTOR) proteins have a primary role as acting via transcriptional repression of the direct FLOWERING LOCUS T (FT) activator CONSTANS (CO). Our previous study indicated that one of CDF homologs, OsDOf12, was involved in photoperiodic flowering. However, the functional characterization of other rice CDF like genes is still in progress. Here, we characterized the function of OsDof4 in rice. RESULTS: Phylogenic analysis indicated that OsDof4 is closely clustered into the same subgroup with CDFs and OsDof12. The subcellular localization experiment and transcriptional activity assay suggested that OsDof4 may function as a transcription factor. The diurnal expression pattern indicated that OsDof4 was regulated by endogenous circadian clock. Overexpression of OsDof4 led to earlier flowering under natural long-day field conditions (NLDs) and late flowering under natural short-day field conditions (NSDs), respectively. We compared the expression level of key floral genes in vector line and OsDof4-ox lines grown under long-day conditions (LDs) and short-day conditions (SDs). Real-time q-PCR results demonstrated that under LDs, Hd3a, RFT1 and Ehd1 were up-regulated whereas under SDs they were down-regulated. Hd1 was down-regulated at dusk period independent of photoperiods. CONCLUSIONS: Taken these results together, we may speculate that the abnormal flowering responses in OsDof4-ox plants under LDs and SDs might be mediated by Ehd1 and Hd1. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-017-1109-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-56490772017-10-26 Constitutive expression of OsDof4, encoding a C(2)-C(2) zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.) Wu, Qi Liu, Xue Yin, Dedong Yuan, Hua Xie, Qi Zhao, Xianfeng Li, Xiaobing Zhu, Lihuang Li, Shigui Li, Dayong BMC Plant Biol Research Article BACKGROUND: Dof (DNA binding with one finger) proteins, a class of plant-specific transcription factors which contain a conserved C2-C2-type zinc finger domain, are involved in many fundamental processes. In the Arabidopsis photoperiod response pathway, CDF (CYCLING DOF FACTOR) proteins have a primary role as acting via transcriptional repression of the direct FLOWERING LOCUS T (FT) activator CONSTANS (CO). Our previous study indicated that one of CDF homologs, OsDOf12, was involved in photoperiodic flowering. However, the functional characterization of other rice CDF like genes is still in progress. Here, we characterized the function of OsDof4 in rice. RESULTS: Phylogenic analysis indicated that OsDof4 is closely clustered into the same subgroup with CDFs and OsDof12. The subcellular localization experiment and transcriptional activity assay suggested that OsDof4 may function as a transcription factor. The diurnal expression pattern indicated that OsDof4 was regulated by endogenous circadian clock. Overexpression of OsDof4 led to earlier flowering under natural long-day field conditions (NLDs) and late flowering under natural short-day field conditions (NSDs), respectively. We compared the expression level of key floral genes in vector line and OsDof4-ox lines grown under long-day conditions (LDs) and short-day conditions (SDs). Real-time q-PCR results demonstrated that under LDs, Hd3a, RFT1 and Ehd1 were up-regulated whereas under SDs they were down-regulated. Hd1 was down-regulated at dusk period independent of photoperiods. CONCLUSIONS: Taken these results together, we may speculate that the abnormal flowering responses in OsDof4-ox plants under LDs and SDs might be mediated by Ehd1 and Hd1. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-017-1109-0) contains supplementary material, which is available to authorized users. BioMed Central 2017-10-19 /pmc/articles/PMC5649077/ /pubmed/29052517 http://dx.doi.org/10.1186/s12870-017-1109-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wu, Qi
Liu, Xue
Yin, Dedong
Yuan, Hua
Xie, Qi
Zhao, Xianfeng
Li, Xiaobing
Zhu, Lihuang
Li, Shigui
Li, Dayong
Constitutive expression of OsDof4, encoding a C(2)-C(2) zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.)
title Constitutive expression of OsDof4, encoding a C(2)-C(2) zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.)
title_full Constitutive expression of OsDof4, encoding a C(2)-C(2) zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.)
title_fullStr Constitutive expression of OsDof4, encoding a C(2)-C(2) zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.)
title_full_unstemmed Constitutive expression of OsDof4, encoding a C(2)-C(2) zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.)
title_short Constitutive expression of OsDof4, encoding a C(2)-C(2) zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (Oryza sativa L.)
title_sort constitutive expression of osdof4, encoding a c(2)-c(2) zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice (oryza sativa l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649077/
https://www.ncbi.nlm.nih.gov/pubmed/29052517
http://dx.doi.org/10.1186/s12870-017-1109-0
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