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Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat
BACKGROUND: Toll-like receptor 4 (TLR4), an innate immune receptor, is suspected to play a key role in the postprandial inflammation that is induced by a high-fat meal rich in saturated fatty acids (SFA). Our objective was to test this hypothesis by using a specific competitive inhibitor of TLR4 (IN...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649083/ https://www.ncbi.nlm.nih.gov/pubmed/29075306 http://dx.doi.org/10.1186/s12986-017-0220-4 |
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author | Hermier, Dominique Mathé, Véronique Lan, Annaïg Santini, Clélia Quignard-Boulangé, Annie Huneau, Jean-François Mariotti, François |
author_facet | Hermier, Dominique Mathé, Véronique Lan, Annaïg Santini, Clélia Quignard-Boulangé, Annie Huneau, Jean-François Mariotti, François |
author_sort | Hermier, Dominique |
collection | PubMed |
description | BACKGROUND: Toll-like receptor 4 (TLR4), an innate immune receptor, is suspected to play a key role in the postprandial inflammation that is induced by a high-fat meal rich in saturated fatty acids (SFA). Our objective was to test this hypothesis by using a specific competitive inhibitor of TLR4 (INH) vs vehicle (VEH) administered immediately before a high-SFA meal in rats. METHODS: First, in a cross-over kinetic study of 12 rats receiving INH and VEH i.v. 10 min before the test meal, we measured plasma inflammatory and vascular markers for 6 h. Then, in 20 rats, 3 h after INH or VEH followed by the test meal (parallel study), we measured the mRNA level of a set of cytokines (Il1-β, Il-6, Tnfα, Mcp-1, Pai-1), and of Tlr4 and Tlr2 in the adipose tissue and the liver, and that of adhesion molecules (Icam-1 and Vcam-1) in the aorta. RESULTS: Plasma IL-6 and PAI-1 increased >4-fold at 3–4 h after test-meals, very similarly after INH as compared to VEH. The expression of TLR2 and of all measured cytokine genes in the adipose tissue was dramatically higher after INH (vs VEH). In the liver, gene expression of Il1-β, Tnfα, Mcp-1 and Tlr2, was also higher after INH, though more moderately, whereas that of Il-6 and Pai-1 was similar between groups. INH did not affect mRNA level of Icam-1 and Vcam-1 in the aorta. CONCLUSION: TLR4 activation is not specifically required to mediate systemic postprandial inflammation and we propose that TLR2 and TLR4 exert a dual and interdependent mediation of the postprandial inflammatory response, at least in the adipose tissue. |
format | Online Article Text |
id | pubmed-5649083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56490832017-10-26 Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat Hermier, Dominique Mathé, Véronique Lan, Annaïg Santini, Clélia Quignard-Boulangé, Annie Huneau, Jean-François Mariotti, François Nutr Metab (Lond) Research BACKGROUND: Toll-like receptor 4 (TLR4), an innate immune receptor, is suspected to play a key role in the postprandial inflammation that is induced by a high-fat meal rich in saturated fatty acids (SFA). Our objective was to test this hypothesis by using a specific competitive inhibitor of TLR4 (INH) vs vehicle (VEH) administered immediately before a high-SFA meal in rats. METHODS: First, in a cross-over kinetic study of 12 rats receiving INH and VEH i.v. 10 min before the test meal, we measured plasma inflammatory and vascular markers for 6 h. Then, in 20 rats, 3 h after INH or VEH followed by the test meal (parallel study), we measured the mRNA level of a set of cytokines (Il1-β, Il-6, Tnfα, Mcp-1, Pai-1), and of Tlr4 and Tlr2 in the adipose tissue and the liver, and that of adhesion molecules (Icam-1 and Vcam-1) in the aorta. RESULTS: Plasma IL-6 and PAI-1 increased >4-fold at 3–4 h after test-meals, very similarly after INH as compared to VEH. The expression of TLR2 and of all measured cytokine genes in the adipose tissue was dramatically higher after INH (vs VEH). In the liver, gene expression of Il1-β, Tnfα, Mcp-1 and Tlr2, was also higher after INH, though more moderately, whereas that of Il-6 and Pai-1 was similar between groups. INH did not affect mRNA level of Icam-1 and Vcam-1 in the aorta. CONCLUSION: TLR4 activation is not specifically required to mediate systemic postprandial inflammation and we propose that TLR2 and TLR4 exert a dual and interdependent mediation of the postprandial inflammatory response, at least in the adipose tissue. BioMed Central 2017-10-19 /pmc/articles/PMC5649083/ /pubmed/29075306 http://dx.doi.org/10.1186/s12986-017-0220-4 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Hermier, Dominique Mathé, Véronique Lan, Annaïg Santini, Clélia Quignard-Boulangé, Annie Huneau, Jean-François Mariotti, François Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat |
title | Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat |
title_full | Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat |
title_fullStr | Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat |
title_full_unstemmed | Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat |
title_short | Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat |
title_sort | postprandial low-grade inflammation does not specifically require tlr4 activation in the rat |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649083/ https://www.ncbi.nlm.nih.gov/pubmed/29075306 http://dx.doi.org/10.1186/s12986-017-0220-4 |
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