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Gastric TFF1 Expression from Acute to Chronic Helicobacter Infection

TFF1, a mucin-associated secreted peptide of gastric mucous cells, is known as a protective agent for stomach epithelium under different stimuli, but its role upon Helicobacter infection is still not clear. In this paper we characterized TFFs expression, with particular attention to TFF1, under Heli...

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Autores principales: Esposito, Roberta, Morello, Silvana, Vllahu, Megi, Eletto, Daniela, Porta, Amalia, Tosco, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649190/
https://www.ncbi.nlm.nih.gov/pubmed/29085807
http://dx.doi.org/10.3389/fcimb.2017.00434
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author Esposito, Roberta
Morello, Silvana
Vllahu, Megi
Eletto, Daniela
Porta, Amalia
Tosco, Alessandra
author_facet Esposito, Roberta
Morello, Silvana
Vllahu, Megi
Eletto, Daniela
Porta, Amalia
Tosco, Alessandra
author_sort Esposito, Roberta
collection PubMed
description TFF1, a mucin-associated secreted peptide of gastric mucous cells, is known as a protective agent for stomach epithelium under different stimuli, but its role upon Helicobacter infection is still not clear. In this paper we characterized TFFs expression, with particular attention to TFF1, under Helicobacter infection in gastric cell lines. A mouse model was used to distinguish TFF1 mRNA expression between acute and chronic stages of Helicobacter infection. Our results show that TFF1 expression is induced in infected cells; in addition, the inflammatory response upon Helicobacter infection is inversely associated to pre-existing TFF1 protein levels. In infected mice, TFF1 is initially upregulated in gastric antrum in the acute phase of infection, along with IL-1β and IL-6. Then, expression of TFF1 is gradually silenced when the infection becomes chronic and IFN-γ, CXCL5, and CXCL15 reach higher levels. Our data suggest that TFF1 might help cells to counteract bacteria colonization and the development of a chronic inflammation.
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spelling pubmed-56491902017-10-30 Gastric TFF1 Expression from Acute to Chronic Helicobacter Infection Esposito, Roberta Morello, Silvana Vllahu, Megi Eletto, Daniela Porta, Amalia Tosco, Alessandra Front Cell Infect Microbiol Microbiology TFF1, a mucin-associated secreted peptide of gastric mucous cells, is known as a protective agent for stomach epithelium under different stimuli, but its role upon Helicobacter infection is still not clear. In this paper we characterized TFFs expression, with particular attention to TFF1, under Helicobacter infection in gastric cell lines. A mouse model was used to distinguish TFF1 mRNA expression between acute and chronic stages of Helicobacter infection. Our results show that TFF1 expression is induced in infected cells; in addition, the inflammatory response upon Helicobacter infection is inversely associated to pre-existing TFF1 protein levels. In infected mice, TFF1 is initially upregulated in gastric antrum in the acute phase of infection, along with IL-1β and IL-6. Then, expression of TFF1 is gradually silenced when the infection becomes chronic and IFN-γ, CXCL5, and CXCL15 reach higher levels. Our data suggest that TFF1 might help cells to counteract bacteria colonization and the development of a chronic inflammation. Frontiers Media S.A. 2017-10-09 /pmc/articles/PMC5649190/ /pubmed/29085807 http://dx.doi.org/10.3389/fcimb.2017.00434 Text en Copyright © 2017 Esposito, Morello, Vllahu, Eletto, Porta and Tosco. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Esposito, Roberta
Morello, Silvana
Vllahu, Megi
Eletto, Daniela
Porta, Amalia
Tosco, Alessandra
Gastric TFF1 Expression from Acute to Chronic Helicobacter Infection
title Gastric TFF1 Expression from Acute to Chronic Helicobacter Infection
title_full Gastric TFF1 Expression from Acute to Chronic Helicobacter Infection
title_fullStr Gastric TFF1 Expression from Acute to Chronic Helicobacter Infection
title_full_unstemmed Gastric TFF1 Expression from Acute to Chronic Helicobacter Infection
title_short Gastric TFF1 Expression from Acute to Chronic Helicobacter Infection
title_sort gastric tff1 expression from acute to chronic helicobacter infection
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649190/
https://www.ncbi.nlm.nih.gov/pubmed/29085807
http://dx.doi.org/10.3389/fcimb.2017.00434
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