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Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity

This study evaluated the anesthetic potential of thymol and carvacrol, and their influence on acetylcholinesterase (AChE) activity in the muscle and brain of silver catfish (Rhamdia quelen). The AChE activity of S-(+)-linalool was also evaluated. We subsequently assessed the effects of thymol and S-...

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Autores principales: Bianchini, A.E., Garlet, Q.I., da Cunha, J.A., Bandeira, G., Brusque, I.C.M., Salbego, J., Heinzmann, B.M., Baldisserotto, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649867/
https://www.ncbi.nlm.nih.gov/pubmed/29069225
http://dx.doi.org/10.1590/1414-431X20176346
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author Bianchini, A.E.
Garlet, Q.I.
da Cunha, J.A.
Bandeira, G.
Brusque, I.C.M.
Salbego, J.
Heinzmann, B.M.
Baldisserotto, B.
author_facet Bianchini, A.E.
Garlet, Q.I.
da Cunha, J.A.
Bandeira, G.
Brusque, I.C.M.
Salbego, J.
Heinzmann, B.M.
Baldisserotto, B.
author_sort Bianchini, A.E.
collection PubMed
description This study evaluated the anesthetic potential of thymol and carvacrol, and their influence on acetylcholinesterase (AChE) activity in the muscle and brain of silver catfish (Rhamdia quelen). The AChE activity of S-(+)-linalool was also evaluated. We subsequently assessed the effects of thymol and S-(+)-linalool on the GABAergic system. Fish were exposed to thymol and carvacrol (25, 50, 75, and 100 mg/L) to evaluate time for anesthesia and recovery. Both compounds induced sedation at 25 mg/L and anesthesia with 50–100 mg/L. However, fish exposed to carvacrol presented strong muscle contractions and mortality. AChE activity was increased in the brain of fish at 50 mg/L carvacrol and 100 mg/L thymol, and decreased in the muscle at 100 mg/L carvacrol. S-(+)-linalool did not alter AChE activity. Anesthesia with thymol was reversed by exposure to picrotoxin (GABA(A) antagonist), similar to the positive control propofol, but was not reversed by flumazenil (antagonist of benzodiazepine binding site), as observed for the positive control diazepam. Picrotoxin did not reverse the effect of S-(+)-linalool. Thymol exposure at 50 mg/L is more suitable than carvacrol for anesthesia in silver catfish, because this concentration did not cause any mortality or interference with AChE activity. Thymol interacted with GABA(A) receptors, but not with the GABA(A)/benzodiazepine site. In contrast, S-(+)-linalool did not act in GABA(A) receptors in silver catfish.
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spelling pubmed-56498672017-10-31 Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity Bianchini, A.E. Garlet, Q.I. da Cunha, J.A. Bandeira, G. Brusque, I.C.M. Salbego, J. Heinzmann, B.M. Baldisserotto, B. Braz J Med Biol Res Research Articles This study evaluated the anesthetic potential of thymol and carvacrol, and their influence on acetylcholinesterase (AChE) activity in the muscle and brain of silver catfish (Rhamdia quelen). The AChE activity of S-(+)-linalool was also evaluated. We subsequently assessed the effects of thymol and S-(+)-linalool on the GABAergic system. Fish were exposed to thymol and carvacrol (25, 50, 75, and 100 mg/L) to evaluate time for anesthesia and recovery. Both compounds induced sedation at 25 mg/L and anesthesia with 50–100 mg/L. However, fish exposed to carvacrol presented strong muscle contractions and mortality. AChE activity was increased in the brain of fish at 50 mg/L carvacrol and 100 mg/L thymol, and decreased in the muscle at 100 mg/L carvacrol. S-(+)-linalool did not alter AChE activity. Anesthesia with thymol was reversed by exposure to picrotoxin (GABA(A) antagonist), similar to the positive control propofol, but was not reversed by flumazenil (antagonist of benzodiazepine binding site), as observed for the positive control diazepam. Picrotoxin did not reverse the effect of S-(+)-linalool. Thymol exposure at 50 mg/L is more suitable than carvacrol for anesthesia in silver catfish, because this concentration did not cause any mortality or interference with AChE activity. Thymol interacted with GABA(A) receptors, but not with the GABA(A)/benzodiazepine site. In contrast, S-(+)-linalool did not act in GABA(A) receptors in silver catfish. Associação Brasileira de Divulgação Científica 2017-10-19 /pmc/articles/PMC5649867/ /pubmed/29069225 http://dx.doi.org/10.1590/1414-431X20176346 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Bianchini, A.E.
Garlet, Q.I.
da Cunha, J.A.
Bandeira, G.
Brusque, I.C.M.
Salbego, J.
Heinzmann, B.M.
Baldisserotto, B.
Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity
title Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity
title_full Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity
title_fullStr Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity
title_full_unstemmed Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity
title_short Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity
title_sort monoterpenoids (thymol, carvacrol and s-(+)-linalool) with anesthetic activity in silver catfish (rhamdia quelen): evaluation of acetylcholinesterase and gabaergic activity
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649867/
https://www.ncbi.nlm.nih.gov/pubmed/29069225
http://dx.doi.org/10.1590/1414-431X20176346
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