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Solea senegalensis sperm cryopreservation: New insights on sperm quality
Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650144/ https://www.ncbi.nlm.nih.gov/pubmed/29053706 http://dx.doi.org/10.1371/journal.pone.0186542 |
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author | Riesco, Marta F. Oliveira, Catarina Soares, Florbela Gavaia, Paulo J. Dinis, María T. Cabrita, Elsa |
author_facet | Riesco, Marta F. Oliveira, Catarina Soares, Florbela Gavaia, Paulo J. Dinis, María T. Cabrita, Elsa |
author_sort | Riesco, Marta F. |
collection | PubMed |
description | Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed. In the present study, motility and lipid peroxidation were performed as specific tests allowing us to discard cryopreservation conditions such as methanol as internal cryoprotectant and bovine serum albumin as external cryoprotectant. In addition, a caspase 3/7 detection by flow cytometry was performed to analyze apoptosis activity in the best selected conditions. Moreover, new highly sensitive tests based on transcript number detection have recently been described in fish sperm cryopreservation. For this reason, a transcript level detection assay was performed on certain oxidative and chaperone genes related to fertilization ability and embryo development (hsp70, hsp90BB, hsp90AA, gpx) to select the best cryopreservation conditions. DMSO+ egg yolk proved to be the best cryoprotectant combination in terms of transcript level. This study describes an optimized cryopreservation protocol for Solea senegalensis sperm demonstrating for the first time that transcript degradation is the most sensitive predictor of cell status in this species after cryopreservation. |
format | Online Article Text |
id | pubmed-5650144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56501442017-11-03 Solea senegalensis sperm cryopreservation: New insights on sperm quality Riesco, Marta F. Oliveira, Catarina Soares, Florbela Gavaia, Paulo J. Dinis, María T. Cabrita, Elsa PLoS One Research Article Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed. In the present study, motility and lipid peroxidation were performed as specific tests allowing us to discard cryopreservation conditions such as methanol as internal cryoprotectant and bovine serum albumin as external cryoprotectant. In addition, a caspase 3/7 detection by flow cytometry was performed to analyze apoptosis activity in the best selected conditions. Moreover, new highly sensitive tests based on transcript number detection have recently been described in fish sperm cryopreservation. For this reason, a transcript level detection assay was performed on certain oxidative and chaperone genes related to fertilization ability and embryo development (hsp70, hsp90BB, hsp90AA, gpx) to select the best cryopreservation conditions. DMSO+ egg yolk proved to be the best cryoprotectant combination in terms of transcript level. This study describes an optimized cryopreservation protocol for Solea senegalensis sperm demonstrating for the first time that transcript degradation is the most sensitive predictor of cell status in this species after cryopreservation. Public Library of Science 2017-10-20 /pmc/articles/PMC5650144/ /pubmed/29053706 http://dx.doi.org/10.1371/journal.pone.0186542 Text en © 2017 Riesco et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Riesco, Marta F. Oliveira, Catarina Soares, Florbela Gavaia, Paulo J. Dinis, María T. Cabrita, Elsa Solea senegalensis sperm cryopreservation: New insights on sperm quality |
title | Solea senegalensis sperm cryopreservation: New insights on sperm quality |
title_full | Solea senegalensis sperm cryopreservation: New insights on sperm quality |
title_fullStr | Solea senegalensis sperm cryopreservation: New insights on sperm quality |
title_full_unstemmed | Solea senegalensis sperm cryopreservation: New insights on sperm quality |
title_short | Solea senegalensis sperm cryopreservation: New insights on sperm quality |
title_sort | solea senegalensis sperm cryopreservation: new insights on sperm quality |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650144/ https://www.ncbi.nlm.nih.gov/pubmed/29053706 http://dx.doi.org/10.1371/journal.pone.0186542 |
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