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Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism

Precise characterization of biological processes critical to proliferation and metastasis of colorectal cancer should facilitate the development of diagnostic and prognostic biomarkers as well as novel treatments. Using mRNA-Seq, we examined the protein coding messenger RNA (mRNA) expression profile...

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Autores principales: Xu, Lai, Wang, Rong, Ziegelbauer, Joseph, Wu, Wells W., Shen, Rong-Fong, Juhl, Hartmut, Zhang, Yaqin, Pelosof, Lorraine, Rosenberg, Amy S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650373/
https://www.ncbi.nlm.nih.gov/pubmed/29088818
http://dx.doi.org/10.18632/oncotarget.20345
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author Xu, Lai
Wang, Rong
Ziegelbauer, Joseph
Wu, Wells W.
Shen, Rong-Fong
Juhl, Hartmut
Zhang, Yaqin
Pelosof, Lorraine
Rosenberg, Amy S.
author_facet Xu, Lai
Wang, Rong
Ziegelbauer, Joseph
Wu, Wells W.
Shen, Rong-Fong
Juhl, Hartmut
Zhang, Yaqin
Pelosof, Lorraine
Rosenberg, Amy S.
author_sort Xu, Lai
collection PubMed
description Precise characterization of biological processes critical to proliferation and metastasis of colorectal cancer should facilitate the development of diagnostic and prognostic biomarkers as well as novel treatments. Using mRNA-Seq, we examined the protein coding messenger RNA (mRNA) expression profiles across different histologically defined stages of primary colon cancers and compared them to their patient matched normal tissue controls. In comparing 79 colorectal cancers to their matched normal mucosa, tumors were distinguished from normal non-malignant tissues not only in the upregulation of biological processes pertaining to cell proliferation, inflammation, and tissue remodeling, but even more strikingly, in downregulated biological processes including fatty acid beta oxidization for ATP production and epithelial cell differentiation and function. A network analysis of deregulated genes revealed newly described cancer networks and putative hub genes. Taken together, our findings suggest that, within an inflammatory microenvironment, invasive, dedifferentiated and rapidly dividing tumor cells divert the oxidation of fatty acids and lipids from energy production into lipid components of cell membranes and organelles to support tumor proliferation. A gene co-expression network analysis provides a clear and broad picture of biological pathways in tumors that may significantly enhance or supplant current histopathologic studies.
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spelling pubmed-56503732017-10-30 Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism Xu, Lai Wang, Rong Ziegelbauer, Joseph Wu, Wells W. Shen, Rong-Fong Juhl, Hartmut Zhang, Yaqin Pelosof, Lorraine Rosenberg, Amy S. Oncotarget Research Paper Precise characterization of biological processes critical to proliferation and metastasis of colorectal cancer should facilitate the development of diagnostic and prognostic biomarkers as well as novel treatments. Using mRNA-Seq, we examined the protein coding messenger RNA (mRNA) expression profiles across different histologically defined stages of primary colon cancers and compared them to their patient matched normal tissue controls. In comparing 79 colorectal cancers to their matched normal mucosa, tumors were distinguished from normal non-malignant tissues not only in the upregulation of biological processes pertaining to cell proliferation, inflammation, and tissue remodeling, but even more strikingly, in downregulated biological processes including fatty acid beta oxidization for ATP production and epithelial cell differentiation and function. A network analysis of deregulated genes revealed newly described cancer networks and putative hub genes. Taken together, our findings suggest that, within an inflammatory microenvironment, invasive, dedifferentiated and rapidly dividing tumor cells divert the oxidation of fatty acids and lipids from energy production into lipid components of cell membranes and organelles to support tumor proliferation. A gene co-expression network analysis provides a clear and broad picture of biological pathways in tumors that may significantly enhance or supplant current histopathologic studies. Impact Journals LLC 2017-08-18 /pmc/articles/PMC5650373/ /pubmed/29088818 http://dx.doi.org/10.18632/oncotarget.20345 Text en Copyright: © 2017 Xu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Xu, Lai
Wang, Rong
Ziegelbauer, Joseph
Wu, Wells W.
Shen, Rong-Fong
Juhl, Hartmut
Zhang, Yaqin
Pelosof, Lorraine
Rosenberg, Amy S.
Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism
title Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism
title_full Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism
title_fullStr Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism
title_full_unstemmed Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism
title_short Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism
title_sort transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650373/
https://www.ncbi.nlm.nih.gov/pubmed/29088818
http://dx.doi.org/10.18632/oncotarget.20345
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