Cargando…
Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism
Precise characterization of biological processes critical to proliferation and metastasis of colorectal cancer should facilitate the development of diagnostic and prognostic biomarkers as well as novel treatments. Using mRNA-Seq, we examined the protein coding messenger RNA (mRNA) expression profile...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650373/ https://www.ncbi.nlm.nih.gov/pubmed/29088818 http://dx.doi.org/10.18632/oncotarget.20345 |
_version_ | 1783272696399790080 |
---|---|
author | Xu, Lai Wang, Rong Ziegelbauer, Joseph Wu, Wells W. Shen, Rong-Fong Juhl, Hartmut Zhang, Yaqin Pelosof, Lorraine Rosenberg, Amy S. |
author_facet | Xu, Lai Wang, Rong Ziegelbauer, Joseph Wu, Wells W. Shen, Rong-Fong Juhl, Hartmut Zhang, Yaqin Pelosof, Lorraine Rosenberg, Amy S. |
author_sort | Xu, Lai |
collection | PubMed |
description | Precise characterization of biological processes critical to proliferation and metastasis of colorectal cancer should facilitate the development of diagnostic and prognostic biomarkers as well as novel treatments. Using mRNA-Seq, we examined the protein coding messenger RNA (mRNA) expression profiles across different histologically defined stages of primary colon cancers and compared them to their patient matched normal tissue controls. In comparing 79 colorectal cancers to their matched normal mucosa, tumors were distinguished from normal non-malignant tissues not only in the upregulation of biological processes pertaining to cell proliferation, inflammation, and tissue remodeling, but even more strikingly, in downregulated biological processes including fatty acid beta oxidization for ATP production and epithelial cell differentiation and function. A network analysis of deregulated genes revealed newly described cancer networks and putative hub genes. Taken together, our findings suggest that, within an inflammatory microenvironment, invasive, dedifferentiated and rapidly dividing tumor cells divert the oxidation of fatty acids and lipids from energy production into lipid components of cell membranes and organelles to support tumor proliferation. A gene co-expression network analysis provides a clear and broad picture of biological pathways in tumors that may significantly enhance or supplant current histopathologic studies. |
format | Online Article Text |
id | pubmed-5650373 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56503732017-10-30 Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism Xu, Lai Wang, Rong Ziegelbauer, Joseph Wu, Wells W. Shen, Rong-Fong Juhl, Hartmut Zhang, Yaqin Pelosof, Lorraine Rosenberg, Amy S. Oncotarget Research Paper Precise characterization of biological processes critical to proliferation and metastasis of colorectal cancer should facilitate the development of diagnostic and prognostic biomarkers as well as novel treatments. Using mRNA-Seq, we examined the protein coding messenger RNA (mRNA) expression profiles across different histologically defined stages of primary colon cancers and compared them to their patient matched normal tissue controls. In comparing 79 colorectal cancers to their matched normal mucosa, tumors were distinguished from normal non-malignant tissues not only in the upregulation of biological processes pertaining to cell proliferation, inflammation, and tissue remodeling, but even more strikingly, in downregulated biological processes including fatty acid beta oxidization for ATP production and epithelial cell differentiation and function. A network analysis of deregulated genes revealed newly described cancer networks and putative hub genes. Taken together, our findings suggest that, within an inflammatory microenvironment, invasive, dedifferentiated and rapidly dividing tumor cells divert the oxidation of fatty acids and lipids from energy production into lipid components of cell membranes and organelles to support tumor proliferation. A gene co-expression network analysis provides a clear and broad picture of biological pathways in tumors that may significantly enhance or supplant current histopathologic studies. Impact Journals LLC 2017-08-18 /pmc/articles/PMC5650373/ /pubmed/29088818 http://dx.doi.org/10.18632/oncotarget.20345 Text en Copyright: © 2017 Xu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xu, Lai Wang, Rong Ziegelbauer, Joseph Wu, Wells W. Shen, Rong-Fong Juhl, Hartmut Zhang, Yaqin Pelosof, Lorraine Rosenberg, Amy S. Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism |
title | Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism |
title_full | Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism |
title_fullStr | Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism |
title_full_unstemmed | Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism |
title_short | Transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism |
title_sort | transcriptome analysis of human colorectal cancer biopsies reveals extensive expression correlations among genes related to cell proliferation, lipid metabolism, immune response and collagen catabolism |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650373/ https://www.ncbi.nlm.nih.gov/pubmed/29088818 http://dx.doi.org/10.18632/oncotarget.20345 |
work_keys_str_mv | AT xulai transcriptomeanalysisofhumancolorectalcancerbiopsiesrevealsextensiveexpressioncorrelationsamonggenesrelatedtocellproliferationlipidmetabolismimmuneresponseandcollagencatabolism AT wangrong transcriptomeanalysisofhumancolorectalcancerbiopsiesrevealsextensiveexpressioncorrelationsamonggenesrelatedtocellproliferationlipidmetabolismimmuneresponseandcollagencatabolism AT ziegelbauerjoseph transcriptomeanalysisofhumancolorectalcancerbiopsiesrevealsextensiveexpressioncorrelationsamonggenesrelatedtocellproliferationlipidmetabolismimmuneresponseandcollagencatabolism AT wuwellsw transcriptomeanalysisofhumancolorectalcancerbiopsiesrevealsextensiveexpressioncorrelationsamonggenesrelatedtocellproliferationlipidmetabolismimmuneresponseandcollagencatabolism AT shenrongfong transcriptomeanalysisofhumancolorectalcancerbiopsiesrevealsextensiveexpressioncorrelationsamonggenesrelatedtocellproliferationlipidmetabolismimmuneresponseandcollagencatabolism AT juhlhartmut transcriptomeanalysisofhumancolorectalcancerbiopsiesrevealsextensiveexpressioncorrelationsamonggenesrelatedtocellproliferationlipidmetabolismimmuneresponseandcollagencatabolism AT zhangyaqin transcriptomeanalysisofhumancolorectalcancerbiopsiesrevealsextensiveexpressioncorrelationsamonggenesrelatedtocellproliferationlipidmetabolismimmuneresponseandcollagencatabolism AT pelosoflorraine transcriptomeanalysisofhumancolorectalcancerbiopsiesrevealsextensiveexpressioncorrelationsamonggenesrelatedtocellproliferationlipidmetabolismimmuneresponseandcollagencatabolism AT rosenbergamys transcriptomeanalysisofhumancolorectalcancerbiopsiesrevealsextensiveexpressioncorrelationsamonggenesrelatedtocellproliferationlipidmetabolismimmuneresponseandcollagencatabolism |