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Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review
As long as the genetically modified crops are gaining attention globally, their proper approval and commercialization need accurate and reliable diagnostic methods for the transgenic content. These diagnostic techniques are mainly divided into two major groups, i.e., identification of transgenic (1)...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650622/ https://www.ncbi.nlm.nih.gov/pubmed/29085378 http://dx.doi.org/10.3389/fpls.2017.01670 |
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author | Salisu, Ibrahim B. Shahid, Ahmad A. Yaqoob, Amina Ali, Qurban Bajwa, Kamran S. Rao, Abdul Q. Husnain, Tayyab |
author_facet | Salisu, Ibrahim B. Shahid, Ahmad A. Yaqoob, Amina Ali, Qurban Bajwa, Kamran S. Rao, Abdul Q. Husnain, Tayyab |
author_sort | Salisu, Ibrahim B. |
collection | PubMed |
description | As long as the genetically modified crops are gaining attention globally, their proper approval and commercialization need accurate and reliable diagnostic methods for the transgenic content. These diagnostic techniques are mainly divided into two major groups, i.e., identification of transgenic (1) DNA and (2) proteins from GMOs and their products. Conventional methods such as PCR (polymerase chain reaction) and enzyme-linked immunosorbent assay (ELISA) were routinely employed for DNA and protein based quantification respectively. Although, these Techniques (PCR and ELISA) are considered as significantly convenient and productive, but there is need for more advance technologies that allow for high throughput detection and the quantification of GM event as the production of more complex GMO is increasing day by day. Therefore, recent approaches like microarray, capillary gel electrophoresis, digital PCR and next generation sequencing are more promising due to their accuracy and precise detection of transgenic contents. The present article is a brief comparative study of all such detection techniques on the basis of their advent, feasibility, accuracy, and cost effectiveness. However, these emerging technologies have a lot to do with detection of a specific event, contamination of different events and determination of fusion as well as stacked gene protein are the critical issues to be addressed in future. |
format | Online Article Text |
id | pubmed-5650622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56506222017-10-30 Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review Salisu, Ibrahim B. Shahid, Ahmad A. Yaqoob, Amina Ali, Qurban Bajwa, Kamran S. Rao, Abdul Q. Husnain, Tayyab Front Plant Sci Plant Science As long as the genetically modified crops are gaining attention globally, their proper approval and commercialization need accurate and reliable diagnostic methods for the transgenic content. These diagnostic techniques are mainly divided into two major groups, i.e., identification of transgenic (1) DNA and (2) proteins from GMOs and their products. Conventional methods such as PCR (polymerase chain reaction) and enzyme-linked immunosorbent assay (ELISA) were routinely employed for DNA and protein based quantification respectively. Although, these Techniques (PCR and ELISA) are considered as significantly convenient and productive, but there is need for more advance technologies that allow for high throughput detection and the quantification of GM event as the production of more complex GMO is increasing day by day. Therefore, recent approaches like microarray, capillary gel electrophoresis, digital PCR and next generation sequencing are more promising due to their accuracy and precise detection of transgenic contents. The present article is a brief comparative study of all such detection techniques on the basis of their advent, feasibility, accuracy, and cost effectiveness. However, these emerging technologies have a lot to do with detection of a specific event, contamination of different events and determination of fusion as well as stacked gene protein are the critical issues to be addressed in future. Frontiers Media S.A. 2017-10-16 /pmc/articles/PMC5650622/ /pubmed/29085378 http://dx.doi.org/10.3389/fpls.2017.01670 Text en Copyright © 2017 Salisu, Shahid, Yaqoob, Ali, Bajwa, Rao and Husnain. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Salisu, Ibrahim B. Shahid, Ahmad A. Yaqoob, Amina Ali, Qurban Bajwa, Kamran S. Rao, Abdul Q. Husnain, Tayyab Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review |
title | Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review |
title_full | Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review |
title_fullStr | Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review |
title_full_unstemmed | Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review |
title_short | Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review |
title_sort | molecular approaches for high throughput detection and quantification of genetically modified crops: a review |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650622/ https://www.ncbi.nlm.nih.gov/pubmed/29085378 http://dx.doi.org/10.3389/fpls.2017.01670 |
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