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Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer
BACKGROUND: Diagnosis of Non-small Cell Lung Cancer (NSCLC) at an early stage is a daunting challenge due to the deficiency of specific noninvasive markers. MicroRNAs (miRNAs) play important roles in the initiation and progression of NSCLC. Measuring miRNA expression levels could provide a potential...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Avicenna Research Institute
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650736/ https://www.ncbi.nlm.nih.gov/pubmed/29090068 |
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author | Bagheri, Abouzar Khorram Khorshid, Hamid Reza Mowla, Seyed Javad Mohebbi, Hassan Ali Mohammadian, Azam Yaseri, Mehdi Solaymani-Dodaran, Masoud Sherafatian, Masih Tavallaie, Mahmood |
author_facet | Bagheri, Abouzar Khorram Khorshid, Hamid Reza Mowla, Seyed Javad Mohebbi, Hassan Ali Mohammadian, Azam Yaseri, Mehdi Solaymani-Dodaran, Masoud Sherafatian, Masih Tavallaie, Mahmood |
author_sort | Bagheri, Abouzar |
collection | PubMed |
description | BACKGROUND: Diagnosis of Non-small Cell Lung Cancer (NSCLC) at an early stage is a daunting challenge due to the deficiency of specific noninvasive markers. MicroRNAs (miRNAs) play important roles in the initiation and progression of NSCLC. Measuring miRNA expression levels could provide a potential approach for the diagnosis of NSCLC. Our goals were to examine miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 expression levels in tissue and sputum of NSCLC patients and cancer free subjects for molecular diagnosis of NSCLC. METHODS: Relative expressions of miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 were examined with quantitative real-time RT-PCR assay in tissue and sputum obtained from 17 NSCLC patients and 17 controls. RESULTS: miR-3074 was upregulated in tissue samples of NSCLC patients compared with control group. miR-223 was upregulated, miR-212 and SNORD37 were downergulated in sputum samples of patients compared with controls. miR-223 quantification produced 82% sensitivity and 95% specificity with areas under the ROC curve at 0.90 in detection of NSCLC. CONCLUSION: miR-223 clearly discriminated cancer patients from cancer-free subjects and our results suggest that miR-223 could be a diagnostic useful biomarker. The measurement of altered miRNA expression in sputum samples manifested the potential noninvasive approach for detection of lung cancer. |
format | Online Article Text |
id | pubmed-5650736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Avicenna Research Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-56507362017-10-31 Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer Bagheri, Abouzar Khorram Khorshid, Hamid Reza Mowla, Seyed Javad Mohebbi, Hassan Ali Mohammadian, Azam Yaseri, Mehdi Solaymani-Dodaran, Masoud Sherafatian, Masih Tavallaie, Mahmood Avicenna J Med Biotechnol Original Article BACKGROUND: Diagnosis of Non-small Cell Lung Cancer (NSCLC) at an early stage is a daunting challenge due to the deficiency of specific noninvasive markers. MicroRNAs (miRNAs) play important roles in the initiation and progression of NSCLC. Measuring miRNA expression levels could provide a potential approach for the diagnosis of NSCLC. Our goals were to examine miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 expression levels in tissue and sputum of NSCLC patients and cancer free subjects for molecular diagnosis of NSCLC. METHODS: Relative expressions of miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 were examined with quantitative real-time RT-PCR assay in tissue and sputum obtained from 17 NSCLC patients and 17 controls. RESULTS: miR-3074 was upregulated in tissue samples of NSCLC patients compared with control group. miR-223 was upregulated, miR-212 and SNORD37 were downergulated in sputum samples of patients compared with controls. miR-223 quantification produced 82% sensitivity and 95% specificity with areas under the ROC curve at 0.90 in detection of NSCLC. CONCLUSION: miR-223 clearly discriminated cancer patients from cancer-free subjects and our results suggest that miR-223 could be a diagnostic useful biomarker. The measurement of altered miRNA expression in sputum samples manifested the potential noninvasive approach for detection of lung cancer. Avicenna Research Institute 2017 /pmc/articles/PMC5650736/ /pubmed/29090068 Text en Copyright© 2017 Avicenna Research Institute http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Bagheri, Abouzar Khorram Khorshid, Hamid Reza Mowla, Seyed Javad Mohebbi, Hassan Ali Mohammadian, Azam Yaseri, Mehdi Solaymani-Dodaran, Masoud Sherafatian, Masih Tavallaie, Mahmood Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer |
title | Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer |
title_full | Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer |
title_fullStr | Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer |
title_full_unstemmed | Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer |
title_short | Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer |
title_sort | altered mir-223 expression in sputum for diagnosis of non-small cell lung cancer |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650736/ https://www.ncbi.nlm.nih.gov/pubmed/29090068 |
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