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Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer

BACKGROUND: Diagnosis of Non-small Cell Lung Cancer (NSCLC) at an early stage is a daunting challenge due to the deficiency of specific noninvasive markers. MicroRNAs (miRNAs) play important roles in the initiation and progression of NSCLC. Measuring miRNA expression levels could provide a potential...

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Autores principales: Bagheri, Abouzar, Khorram Khorshid, Hamid Reza, Mowla, Seyed Javad, Mohebbi, Hassan Ali, Mohammadian, Azam, Yaseri, Mehdi, Solaymani-Dodaran, Masoud, Sherafatian, Masih, Tavallaie, Mahmood
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650736/
https://www.ncbi.nlm.nih.gov/pubmed/29090068
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author Bagheri, Abouzar
Khorram Khorshid, Hamid Reza
Mowla, Seyed Javad
Mohebbi, Hassan Ali
Mohammadian, Azam
Yaseri, Mehdi
Solaymani-Dodaran, Masoud
Sherafatian, Masih
Tavallaie, Mahmood
author_facet Bagheri, Abouzar
Khorram Khorshid, Hamid Reza
Mowla, Seyed Javad
Mohebbi, Hassan Ali
Mohammadian, Azam
Yaseri, Mehdi
Solaymani-Dodaran, Masoud
Sherafatian, Masih
Tavallaie, Mahmood
author_sort Bagheri, Abouzar
collection PubMed
description BACKGROUND: Diagnosis of Non-small Cell Lung Cancer (NSCLC) at an early stage is a daunting challenge due to the deficiency of specific noninvasive markers. MicroRNAs (miRNAs) play important roles in the initiation and progression of NSCLC. Measuring miRNA expression levels could provide a potential approach for the diagnosis of NSCLC. Our goals were to examine miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 expression levels in tissue and sputum of NSCLC patients and cancer free subjects for molecular diagnosis of NSCLC. METHODS: Relative expressions of miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 were examined with quantitative real-time RT-PCR assay in tissue and sputum obtained from 17 NSCLC patients and 17 controls. RESULTS: miR-3074 was upregulated in tissue samples of NSCLC patients compared with control group. miR-223 was upregulated, miR-212 and SNORD37 were downergulated in sputum samples of patients compared with controls. miR-223 quantification produced 82% sensitivity and 95% specificity with areas under the ROC curve at 0.90 in detection of NSCLC. CONCLUSION: miR-223 clearly discriminated cancer patients from cancer-free subjects and our results suggest that miR-223 could be a diagnostic useful biomarker. The measurement of altered miRNA expression in sputum samples manifested the potential noninvasive approach for detection of lung cancer.
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spelling pubmed-56507362017-10-31 Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer Bagheri, Abouzar Khorram Khorshid, Hamid Reza Mowla, Seyed Javad Mohebbi, Hassan Ali Mohammadian, Azam Yaseri, Mehdi Solaymani-Dodaran, Masoud Sherafatian, Masih Tavallaie, Mahmood Avicenna J Med Biotechnol Original Article BACKGROUND: Diagnosis of Non-small Cell Lung Cancer (NSCLC) at an early stage is a daunting challenge due to the deficiency of specific noninvasive markers. MicroRNAs (miRNAs) play important roles in the initiation and progression of NSCLC. Measuring miRNA expression levels could provide a potential approach for the diagnosis of NSCLC. Our goals were to examine miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 expression levels in tissue and sputum of NSCLC patients and cancer free subjects for molecular diagnosis of NSCLC. METHODS: Relative expressions of miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 were examined with quantitative real-time RT-PCR assay in tissue and sputum obtained from 17 NSCLC patients and 17 controls. RESULTS: miR-3074 was upregulated in tissue samples of NSCLC patients compared with control group. miR-223 was upregulated, miR-212 and SNORD37 were downergulated in sputum samples of patients compared with controls. miR-223 quantification produced 82% sensitivity and 95% specificity with areas under the ROC curve at 0.90 in detection of NSCLC. CONCLUSION: miR-223 clearly discriminated cancer patients from cancer-free subjects and our results suggest that miR-223 could be a diagnostic useful biomarker. The measurement of altered miRNA expression in sputum samples manifested the potential noninvasive approach for detection of lung cancer. Avicenna Research Institute 2017 /pmc/articles/PMC5650736/ /pubmed/29090068 Text en Copyright© 2017 Avicenna Research Institute http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Bagheri, Abouzar
Khorram Khorshid, Hamid Reza
Mowla, Seyed Javad
Mohebbi, Hassan Ali
Mohammadian, Azam
Yaseri, Mehdi
Solaymani-Dodaran, Masoud
Sherafatian, Masih
Tavallaie, Mahmood
Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer
title Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer
title_full Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer
title_fullStr Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer
title_full_unstemmed Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer
title_short Altered miR-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer
title_sort altered mir-223 expression in sputum for diagnosis of non-small cell lung cancer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5650736/
https://www.ncbi.nlm.nih.gov/pubmed/29090068
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