Cargando…

Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria

Purpose: Many antimicrobial medications are available to combat infections. However, the indiscriminate use of antibiotics has produced antibiotic resistance in the case of many bacterial pathogens. This study focuses on the development of nanoparticles (NPs) that enhance the in vitro antibiotic act...

Descripción completa

Detalles Bibliográficos
Autores principales: Esmaeillou, Mahsa, Zarrini, Gholamreza, Ahangarzadeh Rezaee, Mohammad, Shahbazi mojarrad, Javid, Bahadori, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651071/
https://www.ncbi.nlm.nih.gov/pubmed/29071232
http://dx.doi.org/10.15171/apb.2017.058
_version_ 1783272823314186240
author Esmaeillou, Mahsa
Zarrini, Gholamreza
Ahangarzadeh Rezaee, Mohammad
Shahbazi mojarrad, Javid
Bahadori, Ali
author_facet Esmaeillou, Mahsa
Zarrini, Gholamreza
Ahangarzadeh Rezaee, Mohammad
Shahbazi mojarrad, Javid
Bahadori, Ali
author_sort Esmaeillou, Mahsa
collection PubMed
description Purpose: Many antimicrobial medications are available to combat infections. However, the indiscriminate use of antibiotics has produced antibiotic resistance in the case of many bacterial pathogens. This study focuses on the development of nanoparticles (NPs) that enhance the in vitro antibiotic activity of vancomycin against multi-drug resistant (MDR) organisms. Methods: Spherical shaped thioglycolic acid-stabilized silver nanoparticles (TGA-AgNPs) were prepared by using a simple chemical reduction method. Then, vancomycin was conjugated to the terminal carboxyl of TGA in the presence of N-Hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC). Afterwards, the antibacterial activity of these nanoconjugates was examined by using the minimum inhibitory concentration (MIC) assay against MDR bacteria. Results: The rate of vancomycin bound to the AgNPs was 19.6%. The MIC values of vancomycin (Van)-capped AgNPs against tested pathogens were in the range of (3.2, 1.6, 0.8, 0.4, 0.2, 0.1, 0.05, and 0.025 µl/ml). The MIC was 0.1 µg/ml for VRE, MIC≤0.02 µg/ml for MRSE, and 0.05 µg/ml for S. aureus. The MIC corresponded to the MBC for all bacterial species. Conclusion: This study indicated that some antimicrobial agents like vancomycin can be conjugated with AgNPs. This can lead to increased antimicrobial activity against MDR microorganisms.
format Online
Article
Text
id pubmed-5651071
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Tabriz University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-56510712017-10-25 Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria Esmaeillou, Mahsa Zarrini, Gholamreza Ahangarzadeh Rezaee, Mohammad Shahbazi mojarrad, Javid Bahadori, Ali Adv Pharm Bull Short Communication Purpose: Many antimicrobial medications are available to combat infections. However, the indiscriminate use of antibiotics has produced antibiotic resistance in the case of many bacterial pathogens. This study focuses on the development of nanoparticles (NPs) that enhance the in vitro antibiotic activity of vancomycin against multi-drug resistant (MDR) organisms. Methods: Spherical shaped thioglycolic acid-stabilized silver nanoparticles (TGA-AgNPs) were prepared by using a simple chemical reduction method. Then, vancomycin was conjugated to the terminal carboxyl of TGA in the presence of N-Hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC). Afterwards, the antibacterial activity of these nanoconjugates was examined by using the minimum inhibitory concentration (MIC) assay against MDR bacteria. Results: The rate of vancomycin bound to the AgNPs was 19.6%. The MIC values of vancomycin (Van)-capped AgNPs against tested pathogens were in the range of (3.2, 1.6, 0.8, 0.4, 0.2, 0.1, 0.05, and 0.025 µl/ml). The MIC was 0.1 µg/ml for VRE, MIC≤0.02 µg/ml for MRSE, and 0.05 µg/ml for S. aureus. The MIC corresponded to the MBC for all bacterial species. Conclusion: This study indicated that some antimicrobial agents like vancomycin can be conjugated with AgNPs. This can lead to increased antimicrobial activity against MDR microorganisms. Tabriz University of Medical Sciences 2017-09 2017-09-25 /pmc/articles/PMC5651071/ /pubmed/29071232 http://dx.doi.org/10.15171/apb.2017.058 Text en ©2017 The Authors. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
spellingShingle Short Communication
Esmaeillou, Mahsa
Zarrini, Gholamreza
Ahangarzadeh Rezaee, Mohammad
Shahbazi mojarrad, Javid
Bahadori, Ali
Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria
title Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria
title_full Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria
title_fullStr Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria
title_full_unstemmed Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria
title_short Vancomycin Capped with Silver Nanoparticles as an Antibacterial Agent against Multi-Drug Resistance Bacteria
title_sort vancomycin capped with silver nanoparticles as an antibacterial agent against multi-drug resistance bacteria
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651071/
https://www.ncbi.nlm.nih.gov/pubmed/29071232
http://dx.doi.org/10.15171/apb.2017.058
work_keys_str_mv AT esmaeilloumahsa vancomycincappedwithsilvernanoparticlesasanantibacterialagentagainstmultidrugresistancebacteria
AT zarrinigholamreza vancomycincappedwithsilvernanoparticlesasanantibacterialagentagainstmultidrugresistancebacteria
AT ahangarzadehrezaeemohammad vancomycincappedwithsilvernanoparticlesasanantibacterialagentagainstmultidrugresistancebacteria
AT shahbazimojarradjavid vancomycincappedwithsilvernanoparticlesasanantibacterialagentagainstmultidrugresistancebacteria
AT bahadoriali vancomycincappedwithsilvernanoparticlesasanantibacterialagentagainstmultidrugresistancebacteria