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Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering
The development of synthetic biology and metabolic engineering has painted a great future for the bio-based economy, including fuels, chemicals, and drugs produced from renewable feedstocks. With the rapid advance of genome-scale modeling, pathway assembling and genome engineering/editing, our abili...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651080/ https://www.ncbi.nlm.nih.gov/pubmed/29089935 http://dx.doi.org/10.3389/fmicb.2017.02012 |
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author | Liu, Yang Liu, Ye Wang, Meng |
author_facet | Liu, Yang Liu, Ye Wang, Meng |
author_sort | Liu, Yang |
collection | PubMed |
description | The development of synthetic biology and metabolic engineering has painted a great future for the bio-based economy, including fuels, chemicals, and drugs produced from renewable feedstocks. With the rapid advance of genome-scale modeling, pathway assembling and genome engineering/editing, our ability to design and generate microbial cell factories with various phenotype becomes almost limitless. However, our lack of ability to measure and exert precise control over metabolite concentration related phenotypes becomes a bottleneck in metabolic engineering. Genetically encoded small molecule biosensors, which provide the means to couple metabolite concentration to measurable or actionable outputs, are highly promising solutions to the bottleneck. Here we review recent advances in the design, optimization and application of small molecule biosensor in metabolic engineering, with particular focus on optimization strategies for transcription factor (TF) based biosensors. |
format | Online Article Text |
id | pubmed-5651080 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56510802017-10-31 Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering Liu, Yang Liu, Ye Wang, Meng Front Microbiol Microbiology The development of synthetic biology and metabolic engineering has painted a great future for the bio-based economy, including fuels, chemicals, and drugs produced from renewable feedstocks. With the rapid advance of genome-scale modeling, pathway assembling and genome engineering/editing, our ability to design and generate microbial cell factories with various phenotype becomes almost limitless. However, our lack of ability to measure and exert precise control over metabolite concentration related phenotypes becomes a bottleneck in metabolic engineering. Genetically encoded small molecule biosensors, which provide the means to couple metabolite concentration to measurable or actionable outputs, are highly promising solutions to the bottleneck. Here we review recent advances in the design, optimization and application of small molecule biosensor in metabolic engineering, with particular focus on optimization strategies for transcription factor (TF) based biosensors. Frontiers Media S.A. 2017-10-17 /pmc/articles/PMC5651080/ /pubmed/29089935 http://dx.doi.org/10.3389/fmicb.2017.02012 Text en Copyright © 2017 Liu, Liu and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Liu, Yang Liu, Ye Wang, Meng Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
title | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
title_full | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
title_fullStr | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
title_full_unstemmed | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
title_short | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
title_sort | design, optimization and application of small molecule biosensor in metabolic engineering |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651080/ https://www.ncbi.nlm.nih.gov/pubmed/29089935 http://dx.doi.org/10.3389/fmicb.2017.02012 |
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