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Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I

Secondary phosphorylation develops in myocytes expressing phospho-mimetic cardiac troponin I (cTnI) but it is not known whether multiple substitutions (e.g. cTnISDTD and cTnIS4D) cause preferential phosphorylation of the remaining endogenous or the phospho-mimetic cTnI in intact myocytes. Western an...

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Autores principales: Lang, Sarah E., Stevenson, Tamara K., Schatz, Tabea M., Biesiadecki, Brandon J., Westfall, Margaret V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651495/
https://www.ncbi.nlm.nih.gov/pubmed/29071293
http://dx.doi.org/10.1016/j.dib.2017.09.066
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author Lang, Sarah E.
Stevenson, Tamara K.
Schatz, Tabea M.
Biesiadecki, Brandon J.
Westfall, Margaret V.
author_facet Lang, Sarah E.
Stevenson, Tamara K.
Schatz, Tabea M.
Biesiadecki, Brandon J.
Westfall, Margaret V.
author_sort Lang, Sarah E.
collection PubMed
description Secondary phosphorylation develops in myocytes expressing phospho-mimetic cardiac troponin I (cTnI) but it is not known whether multiple substitutions (e.g. cTnISDTD and cTnIS4D) cause preferential phosphorylation of the remaining endogenous or the phospho-mimetic cTnI in intact myocytes. Western analysis was performed to determine whether the FLAG/total cTnI ratios are similar for phosphorylated versus total cTnI in myocytes expressing phospho-mimetic cTnI with Asp(D) substitutions at S43/45 plus S23/24 (cTnIS4D) or T144 (cTnISDTD). Representative Western analysis of phosphorylated S23/24 (p-S23/24) and S150 (p-S150) are presented along with re-probes using an antibody which detects all cTnI (MAB1691 Ab). The level of p-S150 also is compared to results obtained using single S43D and/or S45D phospho-mimetic substitutions. These results are discussed in more detail in Lang et al. [1]
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spelling pubmed-56514952017-10-25 Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I Lang, Sarah E. Stevenson, Tamara K. Schatz, Tabea M. Biesiadecki, Brandon J. Westfall, Margaret V. Data Brief Proteomics and Biochemistry Secondary phosphorylation develops in myocytes expressing phospho-mimetic cardiac troponin I (cTnI) but it is not known whether multiple substitutions (e.g. cTnISDTD and cTnIS4D) cause preferential phosphorylation of the remaining endogenous or the phospho-mimetic cTnI in intact myocytes. Western analysis was performed to determine whether the FLAG/total cTnI ratios are similar for phosphorylated versus total cTnI in myocytes expressing phospho-mimetic cTnI with Asp(D) substitutions at S43/45 plus S23/24 (cTnIS4D) or T144 (cTnISDTD). Representative Western analysis of phosphorylated S23/24 (p-S23/24) and S150 (p-S150) are presented along with re-probes using an antibody which detects all cTnI (MAB1691 Ab). The level of p-S150 also is compared to results obtained using single S43D and/or S45D phospho-mimetic substitutions. These results are discussed in more detail in Lang et al. [1] Elsevier 2017-10-05 /pmc/articles/PMC5651495/ /pubmed/29071293 http://dx.doi.org/10.1016/j.dib.2017.09.066 Text en © 2017 Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Proteomics and Biochemistry
Lang, Sarah E.
Stevenson, Tamara K.
Schatz, Tabea M.
Biesiadecki, Brandon J.
Westfall, Margaret V.
Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I
title Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I
title_full Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I
title_fullStr Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I
title_full_unstemmed Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I
title_short Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I
title_sort secondary phosphorylation in myocytes expressing flag-tagged and non-tagged phospho-mimetic cardiac troponin i
topic Proteomics and Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651495/
https://www.ncbi.nlm.nih.gov/pubmed/29071293
http://dx.doi.org/10.1016/j.dib.2017.09.066
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