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Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients

BACKGROUND: The use of stem cells, including mesenchymal stem cells (MSCs), for regenerative medicine is gaining interest for the clinical benefits so far obtained in patients. This study investigates the use of adipose autologous tissue in combination with platelet-rich plasma (PRP) to improve the...

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Autores principales: Virzì, Francesco, Bianca, Paola, Giammona, Alessandro, Apuzzo, Tiziana, Di Franco, Simone, Mangiapane, Laura Rosa, Colorito, Maria Luisa, Catalano, Dario, Scavo, Emanuela, Nicotra, Annalisa, Benfante, Antonina, Pistone, Giuseppe, Caputo, Valentina, Dieli, Francesco, Pirrello, Roberto, Stassi, Giorgio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651639/
https://www.ncbi.nlm.nih.gov/pubmed/29058626
http://dx.doi.org/10.1186/s13287-017-0690-3
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author Virzì, Francesco
Bianca, Paola
Giammona, Alessandro
Apuzzo, Tiziana
Di Franco, Simone
Mangiapane, Laura Rosa
Colorito, Maria Luisa
Catalano, Dario
Scavo, Emanuela
Nicotra, Annalisa
Benfante, Antonina
Pistone, Giuseppe
Caputo, Valentina
Dieli, Francesco
Pirrello, Roberto
Stassi, Giorgio
author_facet Virzì, Francesco
Bianca, Paola
Giammona, Alessandro
Apuzzo, Tiziana
Di Franco, Simone
Mangiapane, Laura Rosa
Colorito, Maria Luisa
Catalano, Dario
Scavo, Emanuela
Nicotra, Annalisa
Benfante, Antonina
Pistone, Giuseppe
Caputo, Valentina
Dieli, Francesco
Pirrello, Roberto
Stassi, Giorgio
author_sort Virzì, Francesco
collection PubMed
description BACKGROUND: The use of stem cells, including mesenchymal stem cells (MSCs), for regenerative medicine is gaining interest for the clinical benefits so far obtained in patients. This study investigates the use of adipose autologous tissue in combination with platelet-rich plasma (PRP) to improve the clinical outcome of patients affected by systemic sclerosis (SSc). METHODS: Adipose-derived mesenchymal stem cells (AD-MSCs) and PRPs were purified from healthy donors and SSc patients. The multilineage differentiation potential of AD-MSCs and their genotypic–phenotypic features were investigated. A cytokine production profile was evaluated on AD-MSCs and PRPs from both healthy subjects and SSc patients. The adipose tissue-derived cell fraction, the so-called stromal vascular fraction (SVF), was coinjected with PRP in the perioral area of SSc patients. RESULTS: Histopathological and phenotypical analysis of adipose tissue from SSc patients revealed a disorganization of its distinct architecture coupled with an altered cell composition. Although AD-MSCs derived from SSc patients showed high multipotency, they failed to sustain a terminally differentiated progeny. Furthermore, SVFs derived from SSc patients differed from healthy donors in their MSC-like traits coupled with an aberrant cytokine production profile. Finally, the administration of PRP in combination with autologous SVF improved buccal’s rhyme, skin elasticity and vascularization for all of the SSc patients enrolled in this study. CONCLUSIONS: This innovative regenerative therapy could be exploited for the treatment of chronic connective tissue diseases, including SSc. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-017-0690-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-56516392017-10-26 Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients Virzì, Francesco Bianca, Paola Giammona, Alessandro Apuzzo, Tiziana Di Franco, Simone Mangiapane, Laura Rosa Colorito, Maria Luisa Catalano, Dario Scavo, Emanuela Nicotra, Annalisa Benfante, Antonina Pistone, Giuseppe Caputo, Valentina Dieli, Francesco Pirrello, Roberto Stassi, Giorgio Stem Cell Res Ther Research BACKGROUND: The use of stem cells, including mesenchymal stem cells (MSCs), for regenerative medicine is gaining interest for the clinical benefits so far obtained in patients. This study investigates the use of adipose autologous tissue in combination with platelet-rich plasma (PRP) to improve the clinical outcome of patients affected by systemic sclerosis (SSc). METHODS: Adipose-derived mesenchymal stem cells (AD-MSCs) and PRPs were purified from healthy donors and SSc patients. The multilineage differentiation potential of AD-MSCs and their genotypic–phenotypic features were investigated. A cytokine production profile was evaluated on AD-MSCs and PRPs from both healthy subjects and SSc patients. The adipose tissue-derived cell fraction, the so-called stromal vascular fraction (SVF), was coinjected with PRP in the perioral area of SSc patients. RESULTS: Histopathological and phenotypical analysis of adipose tissue from SSc patients revealed a disorganization of its distinct architecture coupled with an altered cell composition. Although AD-MSCs derived from SSc patients showed high multipotency, they failed to sustain a terminally differentiated progeny. Furthermore, SVFs derived from SSc patients differed from healthy donors in their MSC-like traits coupled with an aberrant cytokine production profile. Finally, the administration of PRP in combination with autologous SVF improved buccal’s rhyme, skin elasticity and vascularization for all of the SSc patients enrolled in this study. CONCLUSIONS: This innovative regenerative therapy could be exploited for the treatment of chronic connective tissue diseases, including SSc. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-017-0690-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-10-23 /pmc/articles/PMC5651639/ /pubmed/29058626 http://dx.doi.org/10.1186/s13287-017-0690-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Virzì, Francesco
Bianca, Paola
Giammona, Alessandro
Apuzzo, Tiziana
Di Franco, Simone
Mangiapane, Laura Rosa
Colorito, Maria Luisa
Catalano, Dario
Scavo, Emanuela
Nicotra, Annalisa
Benfante, Antonina
Pistone, Giuseppe
Caputo, Valentina
Dieli, Francesco
Pirrello, Roberto
Stassi, Giorgio
Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients
title Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients
title_full Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients
title_fullStr Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients
title_full_unstemmed Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients
title_short Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients
title_sort combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651639/
https://www.ncbi.nlm.nih.gov/pubmed/29058626
http://dx.doi.org/10.1186/s13287-017-0690-3
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