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A Simple Confocal Microscopy-based Method for Assessing Sperm Movement
In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Developmental Biology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651689/ https://www.ncbi.nlm.nih.gov/pubmed/29082338 http://dx.doi.org/10.12717/DR.2017.21.3.229 |
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author | Kim, Sung Woo Ki, Min Su Kim, Chan-Lan Hwang, In-Sul Jeon, Ik Soo |
author_facet | Kim, Sung Woo Ki, Min Su Kim, Chan-Lan Hwang, In-Sul Jeon, Ik Soo |
author_sort | Kim, Sung Woo |
collection | PubMed |
description | In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11.0±2.3 beats/s (fresh sperm) to 5.7±1.8 beats/s and increased the flagellar bending angle from 19.8°±13.8° (fresh) to 30.6°±15.6°. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques. |
format | Online Article Text |
id | pubmed-5651689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Korean Society of Developmental Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-56516892017-10-27 A Simple Confocal Microscopy-based Method for Assessing Sperm Movement Kim, Sung Woo Ki, Min Su Kim, Chan-Lan Hwang, In-Sul Jeon, Ik Soo Dev Reprod Original Research Paper In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11.0±2.3 beats/s (fresh sperm) to 5.7±1.8 beats/s and increased the flagellar bending angle from 19.8°±13.8° (fresh) to 30.6°±15.6°. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques. The Korean Society of Developmental Biology 2017-09 2017-09-30 /pmc/articles/PMC5651689/ /pubmed/29082338 http://dx.doi.org/10.12717/DR.2017.21.3.229 Text en ⓒ Copyright 2017 The Korean Society of Developmental Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Paper Kim, Sung Woo Ki, Min Su Kim, Chan-Lan Hwang, In-Sul Jeon, Ik Soo A Simple Confocal Microscopy-based Method for Assessing Sperm Movement |
title | A Simple Confocal Microscopy-based Method for Assessing Sperm
Movement |
title_full | A Simple Confocal Microscopy-based Method for Assessing Sperm
Movement |
title_fullStr | A Simple Confocal Microscopy-based Method for Assessing Sperm
Movement |
title_full_unstemmed | A Simple Confocal Microscopy-based Method for Assessing Sperm
Movement |
title_short | A Simple Confocal Microscopy-based Method for Assessing Sperm
Movement |
title_sort | simple confocal microscopy-based method for assessing sperm
movement |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651689/ https://www.ncbi.nlm.nih.gov/pubmed/29082338 http://dx.doi.org/10.12717/DR.2017.21.3.229 |
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