Cargando…

A Simple Confocal Microscopy-based Method for Assessing Sperm Movement

In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Sung Woo, Ki, Min Su, Kim, Chan-Lan, Hwang, In-Sul, Jeon, Ik Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Developmental Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651689/
https://www.ncbi.nlm.nih.gov/pubmed/29082338
http://dx.doi.org/10.12717/DR.2017.21.3.229
_version_ 1783272943171665920
author Kim, Sung Woo
Ki, Min Su
Kim, Chan-Lan
Hwang, In-Sul
Jeon, Ik Soo
author_facet Kim, Sung Woo
Ki, Min Su
Kim, Chan-Lan
Hwang, In-Sul
Jeon, Ik Soo
author_sort Kim, Sung Woo
collection PubMed
description In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11.0±2.3 beats/s (fresh sperm) to 5.7±1.8 beats/s and increased the flagellar bending angle from 19.8°±13.8° (fresh) to 30.6°±15.6°. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques.
format Online
Article
Text
id pubmed-5651689
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The Korean Society of Developmental Biology
record_format MEDLINE/PubMed
spelling pubmed-56516892017-10-27 A Simple Confocal Microscopy-based Method for Assessing Sperm Movement Kim, Sung Woo Ki, Min Su Kim, Chan-Lan Hwang, In-Sul Jeon, Ik Soo Dev Reprod Original Research Paper In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11.0±2.3 beats/s (fresh sperm) to 5.7±1.8 beats/s and increased the flagellar bending angle from 19.8°±13.8° (fresh) to 30.6°±15.6°. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques. The Korean Society of Developmental Biology 2017-09 2017-09-30 /pmc/articles/PMC5651689/ /pubmed/29082338 http://dx.doi.org/10.12717/DR.2017.21.3.229 Text en ⓒ Copyright 2017 The Korean Society of Developmental Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Paper
Kim, Sung Woo
Ki, Min Su
Kim, Chan-Lan
Hwang, In-Sul
Jeon, Ik Soo
A Simple Confocal Microscopy-based Method for Assessing Sperm Movement
title A Simple Confocal Microscopy-based Method for Assessing Sperm Movement
title_full A Simple Confocal Microscopy-based Method for Assessing Sperm Movement
title_fullStr A Simple Confocal Microscopy-based Method for Assessing Sperm Movement
title_full_unstemmed A Simple Confocal Microscopy-based Method for Assessing Sperm Movement
title_short A Simple Confocal Microscopy-based Method for Assessing Sperm Movement
title_sort simple confocal microscopy-based method for assessing sperm movement
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651689/
https://www.ncbi.nlm.nih.gov/pubmed/29082338
http://dx.doi.org/10.12717/DR.2017.21.3.229
work_keys_str_mv AT kimsungwoo asimpleconfocalmicroscopybasedmethodforassessingspermmovement
AT kiminsu asimpleconfocalmicroscopybasedmethodforassessingspermmovement
AT kimchanlan asimpleconfocalmicroscopybasedmethodforassessingspermmovement
AT hwanginsul asimpleconfocalmicroscopybasedmethodforassessingspermmovement
AT jeoniksoo asimpleconfocalmicroscopybasedmethodforassessingspermmovement
AT kimsungwoo simpleconfocalmicroscopybasedmethodforassessingspermmovement
AT kiminsu simpleconfocalmicroscopybasedmethodforassessingspermmovement
AT kimchanlan simpleconfocalmicroscopybasedmethodforassessingspermmovement
AT hwanginsul simpleconfocalmicroscopybasedmethodforassessingspermmovement
AT jeoniksoo simpleconfocalmicroscopybasedmethodforassessingspermmovement