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DnaJC18, a Novel Type III DnaJ Family Protein, is Expressed Specifically in Rat Male Germ Cells
Mammalian spermatogenesis occurs in a precise and coordinated manner in the seminiferous tubules. One of the attempts to understand the detailed biological process during mammalian spermatogenesis at the molecular level has been to identify the testis specific genes followed by study of the testicul...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Society of Developmental Biology
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651690/ https://www.ncbi.nlm.nih.gov/pubmed/29082339 http://dx.doi.org/10.12717/DR.2017.21.3.237 |
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author | Gomes, Cynthia Soh, Jaemog |
author_facet | Gomes, Cynthia Soh, Jaemog |
author_sort | Gomes, Cynthia |
collection | PubMed |
description | Mammalian spermatogenesis occurs in a precise and coordinated manner in the seminiferous tubules. One of the attempts to understand the detailed biological process during mammalian spermatogenesis at the molecular level has been to identify the testis specific genes followed by study of the testicular expression pattern of the genes. From the subtracted cDNA library of rat testis prepared using representational difference analysis (RDA) method, a complimentary DNA clone encoding type III member of a DnaJ family protein, DnaJC18, was cloned (GenBank Accession No. DQ158861). The full-length DnaJC18 cDNA has the longest open reading frame of 357 amino acids. Tissue and developmental Northern blot analysis revealed that the DnaJC18 gene was expressed specifically in testis and began to express from postnatal week 4 testis, respectively. In situ hybridization studies showed that DnaJC18 mRNA was expressed only during the maturation stages of late pachy- tene, round and elongated spermatids of adult rat testis. Western blot analysis with DnaJC18 antibody revealed that 41.2 kDa DnaJC18 protein was detected only in adult testis. Immunohistochemistry study further confirmed that DnaJC18 protein, was expressed in developing germ cells and the result was in concert with the in situ hybridization result. Confocal microscopy with GFP tagged DnaJC18 protein revealed that it was localized in the cytoplasm of cells. Taken together, these results suggested that testis specific DnaJC18, a member of the type III DnaJ protein family, might play a role during germ cell maturation in adult rat testis. |
format | Online Article Text |
id | pubmed-5651690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Korean Society of Developmental Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-56516902017-10-27 DnaJC18, a Novel Type III DnaJ Family Protein, is Expressed Specifically in Rat Male Germ Cells Gomes, Cynthia Soh, Jaemog Dev Reprod Original Research Paper Mammalian spermatogenesis occurs in a precise and coordinated manner in the seminiferous tubules. One of the attempts to understand the detailed biological process during mammalian spermatogenesis at the molecular level has been to identify the testis specific genes followed by study of the testicular expression pattern of the genes. From the subtracted cDNA library of rat testis prepared using representational difference analysis (RDA) method, a complimentary DNA clone encoding type III member of a DnaJ family protein, DnaJC18, was cloned (GenBank Accession No. DQ158861). The full-length DnaJC18 cDNA has the longest open reading frame of 357 amino acids. Tissue and developmental Northern blot analysis revealed that the DnaJC18 gene was expressed specifically in testis and began to express from postnatal week 4 testis, respectively. In situ hybridization studies showed that DnaJC18 mRNA was expressed only during the maturation stages of late pachy- tene, round and elongated spermatids of adult rat testis. Western blot analysis with DnaJC18 antibody revealed that 41.2 kDa DnaJC18 protein was detected only in adult testis. Immunohistochemistry study further confirmed that DnaJC18 protein, was expressed in developing germ cells and the result was in concert with the in situ hybridization result. Confocal microscopy with GFP tagged DnaJC18 protein revealed that it was localized in the cytoplasm of cells. Taken together, these results suggested that testis specific DnaJC18, a member of the type III DnaJ protein family, might play a role during germ cell maturation in adult rat testis. The Korean Society of Developmental Biology 2017-09 2017-09-30 /pmc/articles/PMC5651690/ /pubmed/29082339 http://dx.doi.org/10.12717/DR.2017.21.3.237 Text en ⓒ Copyright 2017 The Korean Society of Developmental Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Paper Gomes, Cynthia Soh, Jaemog DnaJC18, a Novel Type III DnaJ Family Protein, is Expressed Specifically in Rat Male Germ Cells |
title | DnaJC18, a Novel Type III DnaJ Family Protein, is Expressed
Specifically in Rat Male Germ Cells |
title_full | DnaJC18, a Novel Type III DnaJ Family Protein, is Expressed
Specifically in Rat Male Germ Cells |
title_fullStr | DnaJC18, a Novel Type III DnaJ Family Protein, is Expressed
Specifically in Rat Male Germ Cells |
title_full_unstemmed | DnaJC18, a Novel Type III DnaJ Family Protein, is Expressed
Specifically in Rat Male Germ Cells |
title_short | DnaJC18, a Novel Type III DnaJ Family Protein, is Expressed
Specifically in Rat Male Germ Cells |
title_sort | dnajc18, a novel type iii dnaj family protein, is expressed
specifically in rat male germ cells |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651690/ https://www.ncbi.nlm.nih.gov/pubmed/29082339 http://dx.doi.org/10.12717/DR.2017.21.3.237 |
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