Cargando…
A pilot evaluation of whole blood finger-prick sampling for point-of-care HIV viral load measurement: the UNICORN study
There is a global need for HIV viral load point-of-care (PoC) assays to monitor patients receiving antiretroviral therapy. UNICORN was the first study of an off-label protocol using whole blood finger-prick samples tested with and without a simple three minute spin using a clinic-room microcentrifug...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651802/ https://www.ncbi.nlm.nih.gov/pubmed/29057945 http://dx.doi.org/10.1038/s41598-017-13287-2 |
_version_ | 1783272951251992576 |
---|---|
author | Fidler, Sarah Lewis, Heather Meyerowitz, Jodi Kuldanek, Kristin Thornhill, John Muir, David Bonnissent, Alice Timson, Georgina Frater, John |
author_facet | Fidler, Sarah Lewis, Heather Meyerowitz, Jodi Kuldanek, Kristin Thornhill, John Muir, David Bonnissent, Alice Timson, Georgina Frater, John |
author_sort | Fidler, Sarah |
collection | PubMed |
description | There is a global need for HIV viral load point-of-care (PoC) assays to monitor patients receiving antiretroviral therapy. UNICORN was the first study of an off-label protocol using whole blood finger-prick samples tested with and without a simple three minute spin using a clinic-room microcentrifuge. Two PoC assays were evaluated in 40 HIV-positive participants, 20 with detectable and 20 with undetectable plasma viral load (pVL) (<20 copies/ml). Using 100 µl finger-prick blood samples, the Cepheid Xpert HIV-1 Viral Load and HIV-1 Qual cartridges were compared with laboratory pVL assessment (TaqMan, Roche). For participants with undetectable viraemia by TaqMan, there was poor concordance without centrifugation with the TaqMan platform with only 40% ‘undetectable’ using Xpert VL and 25% ‘not detected’ using the Qual assay. After a 3 minute spin, 100% of samples were undetectable using either assay, showing full concordance with the TaqMan assay. Defining a lower limit of detection of 1000 copies/ml when including a spin, there was 100% concordance with the TaqMan platform with strong correlation (rho 0.95 and 0.94; p < 0.0001 for both assays). When including a simple microcentrifugation step, finger-prick PoC testing was a quick and accurate approach for assessing HIV viraemia, with excellent concordance with validated laboratory approaches. |
format | Online Article Text |
id | pubmed-5651802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56518022017-10-26 A pilot evaluation of whole blood finger-prick sampling for point-of-care HIV viral load measurement: the UNICORN study Fidler, Sarah Lewis, Heather Meyerowitz, Jodi Kuldanek, Kristin Thornhill, John Muir, David Bonnissent, Alice Timson, Georgina Frater, John Sci Rep Article There is a global need for HIV viral load point-of-care (PoC) assays to monitor patients receiving antiretroviral therapy. UNICORN was the first study of an off-label protocol using whole blood finger-prick samples tested with and without a simple three minute spin using a clinic-room microcentrifuge. Two PoC assays were evaluated in 40 HIV-positive participants, 20 with detectable and 20 with undetectable plasma viral load (pVL) (<20 copies/ml). Using 100 µl finger-prick blood samples, the Cepheid Xpert HIV-1 Viral Load and HIV-1 Qual cartridges were compared with laboratory pVL assessment (TaqMan, Roche). For participants with undetectable viraemia by TaqMan, there was poor concordance without centrifugation with the TaqMan platform with only 40% ‘undetectable’ using Xpert VL and 25% ‘not detected’ using the Qual assay. After a 3 minute spin, 100% of samples were undetectable using either assay, showing full concordance with the TaqMan assay. Defining a lower limit of detection of 1000 copies/ml when including a spin, there was 100% concordance with the TaqMan platform with strong correlation (rho 0.95 and 0.94; p < 0.0001 for both assays). When including a simple microcentrifugation step, finger-prick PoC testing was a quick and accurate approach for assessing HIV viraemia, with excellent concordance with validated laboratory approaches. Nature Publishing Group UK 2017-10-20 /pmc/articles/PMC5651802/ /pubmed/29057945 http://dx.doi.org/10.1038/s41598-017-13287-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Fidler, Sarah Lewis, Heather Meyerowitz, Jodi Kuldanek, Kristin Thornhill, John Muir, David Bonnissent, Alice Timson, Georgina Frater, John A pilot evaluation of whole blood finger-prick sampling for point-of-care HIV viral load measurement: the UNICORN study |
title | A pilot evaluation of whole blood finger-prick sampling for point-of-care HIV viral load measurement: the UNICORN study |
title_full | A pilot evaluation of whole blood finger-prick sampling for point-of-care HIV viral load measurement: the UNICORN study |
title_fullStr | A pilot evaluation of whole blood finger-prick sampling for point-of-care HIV viral load measurement: the UNICORN study |
title_full_unstemmed | A pilot evaluation of whole blood finger-prick sampling for point-of-care HIV viral load measurement: the UNICORN study |
title_short | A pilot evaluation of whole blood finger-prick sampling for point-of-care HIV viral load measurement: the UNICORN study |
title_sort | pilot evaluation of whole blood finger-prick sampling for point-of-care hiv viral load measurement: the unicorn study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651802/ https://www.ncbi.nlm.nih.gov/pubmed/29057945 http://dx.doi.org/10.1038/s41598-017-13287-2 |
work_keys_str_mv | AT fidlersarah apilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT lewisheather apilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT meyerowitzjodi apilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT kuldanekkristin apilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT thornhilljohn apilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT muirdavid apilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT bonnissentalice apilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT timsongeorgina apilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT fraterjohn apilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT fidlersarah pilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT lewisheather pilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT meyerowitzjodi pilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT kuldanekkristin pilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT thornhilljohn pilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT muirdavid pilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT bonnissentalice pilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT timsongeorgina pilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy AT fraterjohn pilotevaluationofwholebloodfingerpricksamplingforpointofcarehivviralloadmeasurementtheunicornstudy |