Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil

The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between...

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Detalles Bibliográficos
Autores principales: Fischer, Carlo, Torres, Maria C., Patel, Pranav, Moreira-Soto, Andres, Gould, Ernest A., Charrel, Rémi N., de Lamballerie, Xavier, Nogueira, Rita Maria Ribeiro, Sequeira, Patricia C., Rodrigues, Cintia D.S., Kümmerer, Beate M., Drosten, Christian, Landt, Olfert, Bispo de Filippis, Ana Maria, Drexler, Jan Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2017
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652426/
https://www.ncbi.nlm.nih.gov/pubmed/28949285
http://dx.doi.org/10.3201/eid2311.171131
Descripción
Sumario:The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.

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