Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil

The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between...

Descripción completa

Detalles Bibliográficos
Autores principales: Fischer, Carlo, Torres, Maria C., Patel, Pranav, Moreira-Soto, Andres, Gould, Ernest A., Charrel, Rémi N., de Lamballerie, Xavier, Nogueira, Rita Maria Ribeiro, Sequeira, Patricia C., Rodrigues, Cintia D.S., Kümmerer, Beate M., Drosten, Christian, Landt, Olfert, Bispo de Filippis, Ana Maria, Drexler, Jan Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2017
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652426/
https://www.ncbi.nlm.nih.gov/pubmed/28949285
http://dx.doi.org/10.3201/eid2311.171131
_version_ 1783273054846058496
author Fischer, Carlo
Torres, Maria C.
Patel, Pranav
Moreira-Soto, Andres
Gould, Ernest A.
Charrel, Rémi N.
de Lamballerie, Xavier
Nogueira, Rita Maria Ribeiro
Sequeira, Patricia C.
Rodrigues, Cintia D.S.
Kümmerer, Beate M.
Drosten, Christian
Landt, Olfert
Bispo de Filippis, Ana Maria
Drexler, Jan Felix
author_facet Fischer, Carlo
Torres, Maria C.
Patel, Pranav
Moreira-Soto, Andres
Gould, Ernest A.
Charrel, Rémi N.
de Lamballerie, Xavier
Nogueira, Rita Maria Ribeiro
Sequeira, Patricia C.
Rodrigues, Cintia D.S.
Kümmerer, Beate M.
Drosten, Christian
Landt, Olfert
Bispo de Filippis, Ana Maria
Drexler, Jan Felix
author_sort Fischer, Carlo
collection PubMed
description The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.
format Online
Article
Text
id pubmed-5652426
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Centers for Disease Control and Prevention
record_format MEDLINE/PubMed
spelling pubmed-56524262017-11-01 Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil Fischer, Carlo Torres, Maria C. Patel, Pranav Moreira-Soto, Andres Gould, Ernest A. Charrel, Rémi N. de Lamballerie, Xavier Nogueira, Rita Maria Ribeiro Sequeira, Patricia C. Rodrigues, Cintia D.S. Kümmerer, Beate M. Drosten, Christian Landt, Olfert Bispo de Filippis, Ana Maria Drexler, Jan Felix Emerg Infect Dis Dispatch The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting. Centers for Disease Control and Prevention 2017-11 /pmc/articles/PMC5652426/ /pubmed/28949285 http://dx.doi.org/10.3201/eid2311.171131 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Fischer, Carlo
Torres, Maria C.
Patel, Pranav
Moreira-Soto, Andres
Gould, Ernest A.
Charrel, Rémi N.
de Lamballerie, Xavier
Nogueira, Rita Maria Ribeiro
Sequeira, Patricia C.
Rodrigues, Cintia D.S.
Kümmerer, Beate M.
Drosten, Christian
Landt, Olfert
Bispo de Filippis, Ana Maria
Drexler, Jan Felix
Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil
title Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil
title_full Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil
title_fullStr Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil
title_full_unstemmed Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil
title_short Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil
title_sort lineage-specific real-time rt-pcr for yellow fever virus outbreak surveillance, brazil
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652426/
https://www.ncbi.nlm.nih.gov/pubmed/28949285
http://dx.doi.org/10.3201/eid2311.171131
work_keys_str_mv AT fischercarlo lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT torresmariac lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT patelpranav lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT moreirasotoandres lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT gouldernesta lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT charrelremin lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT delamballeriexavier lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT nogueiraritamariaribeiro lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT sequeirapatriciac lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT rodriguescintiads lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT kummererbeatem lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT drostenchristian lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT landtolfert lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT bispodefilippisanamaria lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil
AT drexlerjanfelix lineagespecificrealtimertpcrforyellowfevervirusoutbreaksurveillancebrazil

Ejemplares similares