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Overexpression of SIRT6 attenuates the tumorigenicity of hepatocellular carcinoma cells

OBJECTIVE: This study aimed to explore the effects of overexpression of sirtuin 6 (SIRT6) on the tumorigenicity of hepatocellular carcinoma (HCC) cells METHODS: Stable SIRT6-overexpressed HCC cell lines were established by transfecting SIRT6 plasmid. Soft agar assay and tumor xenograft assay in nude...

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Autores principales: Wang, Yadong, Pan, Teng, Wang, Haiyu, Li, Li, Li, Jiangmin, Zhang, Ding, Yang, Haiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652700/
https://www.ncbi.nlm.nih.gov/pubmed/29100306
http://dx.doi.org/10.18632/oncotarget.19297
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author Wang, Yadong
Pan, Teng
Wang, Haiyu
Li, Li
Li, Jiangmin
Zhang, Ding
Yang, Haiyan
author_facet Wang, Yadong
Pan, Teng
Wang, Haiyu
Li, Li
Li, Jiangmin
Zhang, Ding
Yang, Haiyan
author_sort Wang, Yadong
collection PubMed
description OBJECTIVE: This study aimed to explore the effects of overexpression of sirtuin 6 (SIRT6) on the tumorigenicity of hepatocellular carcinoma (HCC) cells METHODS: Stable SIRT6-overexpressed HCC cell lines were established by transfecting SIRT6 plasmid. Soft agar assay and tumor xenograft assay in nude mice were applied. Flow cytometry was employed to detect cell cycle distribution. Western blotting analysis was used to detect the expression of proteins. RESULTS: Overexpression of SIRT6 attenuated HepG2 and HCCLM3 cells proliferation, colony formation in vitro and tumor formation in nude mice, and resulted in the G1 phase cell cycle arrest. Overexpression of SIRT6 reduced the expression of cyclin D1 and p-ERK proteins in both HepG2 and HCCLM3 cells. CONCLUSION: Overexpression of SIRT6 attenuates the tumorigenicity of HCC cells.
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spelling pubmed-56527002017-11-02 Overexpression of SIRT6 attenuates the tumorigenicity of hepatocellular carcinoma cells Wang, Yadong Pan, Teng Wang, Haiyu Li, Li Li, Jiangmin Zhang, Ding Yang, Haiyan Oncotarget Research Paper OBJECTIVE: This study aimed to explore the effects of overexpression of sirtuin 6 (SIRT6) on the tumorigenicity of hepatocellular carcinoma (HCC) cells METHODS: Stable SIRT6-overexpressed HCC cell lines were established by transfecting SIRT6 plasmid. Soft agar assay and tumor xenograft assay in nude mice were applied. Flow cytometry was employed to detect cell cycle distribution. Western blotting analysis was used to detect the expression of proteins. RESULTS: Overexpression of SIRT6 attenuated HepG2 and HCCLM3 cells proliferation, colony formation in vitro and tumor formation in nude mice, and resulted in the G1 phase cell cycle arrest. Overexpression of SIRT6 reduced the expression of cyclin D1 and p-ERK proteins in both HepG2 and HCCLM3 cells. CONCLUSION: Overexpression of SIRT6 attenuates the tumorigenicity of HCC cells. Impact Journals LLC 2017-07-17 /pmc/articles/PMC5652700/ /pubmed/29100306 http://dx.doi.org/10.18632/oncotarget.19297 Text en Copyright: © 2017 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wang, Yadong
Pan, Teng
Wang, Haiyu
Li, Li
Li, Jiangmin
Zhang, Ding
Yang, Haiyan
Overexpression of SIRT6 attenuates the tumorigenicity of hepatocellular carcinoma cells
title Overexpression of SIRT6 attenuates the tumorigenicity of hepatocellular carcinoma cells
title_full Overexpression of SIRT6 attenuates the tumorigenicity of hepatocellular carcinoma cells
title_fullStr Overexpression of SIRT6 attenuates the tumorigenicity of hepatocellular carcinoma cells
title_full_unstemmed Overexpression of SIRT6 attenuates the tumorigenicity of hepatocellular carcinoma cells
title_short Overexpression of SIRT6 attenuates the tumorigenicity of hepatocellular carcinoma cells
title_sort overexpression of sirt6 attenuates the tumorigenicity of hepatocellular carcinoma cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652700/
https://www.ncbi.nlm.nih.gov/pubmed/29100306
http://dx.doi.org/10.18632/oncotarget.19297
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