Cargando…

The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma

OBJECTIVE: To discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma. METHODS: A total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system....

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Can, He, QiuYan, Zhu, Wei, Fu, ChunYan, Zhou, JianHua, Tao, YongGuang, Liu, Shuang, Xiao, DeSheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652759/
https://www.ncbi.nlm.nih.gov/pubmed/29100365
http://dx.doi.org/10.18632/oncotarget.20330
_version_ 1783273121434828800
author Lu, Can
He, QiuYan
Zhu, Wei
Fu, ChunYan
Zhou, JianHua
Tao, YongGuang
Liu, Shuang
Xiao, DeSheng
author_facet Lu, Can
He, QiuYan
Zhu, Wei
Fu, ChunYan
Zhou, JianHua
Tao, YongGuang
Liu, Shuang
Xiao, DeSheng
author_sort Lu, Can
collection PubMed
description OBJECTIVE: To discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma. METHODS: A total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system. Gel electrophoresis of heteroduplexes was used to analyze immunoglobulin gene rearrangements. RESULTS: A total of 209 cases of B-cell lymphoma, including 69 extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue, 63 diffuse large B-cell lymphomas, 39 follicular lymphomas, 15 small lymphocytic lymphomas, 6 plasmacytomas, 6 mantle cell lymphomas, 7 nodal marginal zone B-cell lymphomas, and 4 lymphoplasmacytoid lymphomas, were examined. Immunoglobulin gene rearrangements were found in all 209 cases, with 93 IGHA, 122 IGHB, 98 IGHC, 167 IGK, 100 IGL, 167 IGHA/B/C, 204 IGH/IGK, 209 IGH/IGK/IGL, 129 IGH+IGK, 81 IGH+IGL, 83 IGK+IGL and 68 IGH+IGK+IGL gene rearrangements. Immunoglobulin gene rearrangements were not found in the 35 cases of reactive lymphoid hyperplasia. IGH and IGK gene rearrangements were mainly found in mantle cell lymphomas, small lymphocytic lymphomas, extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue and diffuse large B-cell lymphomas. The IGH gene rearrangement was mainly found in lymphoplasmacytoid lymphomas and follicular lymphomas. IGK and IGL gene rearrangements were mainly found in plasmocytoma, and the IGK gene rearrangement was mainly found in nodal marginal zone B-cell lymphomas. CONCLUSIONS: The BIOMED-2 standardized immunoglobulin gene rearrangement detection system is an important tool in B-cell lymphoma diagnosis. Analysis of IGH, IGK and IGL gene rearrangements is valuable in confirming the classification of B-cell NHL.
format Online
Article
Text
id pubmed-5652759
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-56527592017-11-02 The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma Lu, Can He, QiuYan Zhu, Wei Fu, ChunYan Zhou, JianHua Tao, YongGuang Liu, Shuang Xiao, DeSheng Oncotarget Research Paper OBJECTIVE: To discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma. METHODS: A total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system. Gel electrophoresis of heteroduplexes was used to analyze immunoglobulin gene rearrangements. RESULTS: A total of 209 cases of B-cell lymphoma, including 69 extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue, 63 diffuse large B-cell lymphomas, 39 follicular lymphomas, 15 small lymphocytic lymphomas, 6 plasmacytomas, 6 mantle cell lymphomas, 7 nodal marginal zone B-cell lymphomas, and 4 lymphoplasmacytoid lymphomas, were examined. Immunoglobulin gene rearrangements were found in all 209 cases, with 93 IGHA, 122 IGHB, 98 IGHC, 167 IGK, 100 IGL, 167 IGHA/B/C, 204 IGH/IGK, 209 IGH/IGK/IGL, 129 IGH+IGK, 81 IGH+IGL, 83 IGK+IGL and 68 IGH+IGK+IGL gene rearrangements. Immunoglobulin gene rearrangements were not found in the 35 cases of reactive lymphoid hyperplasia. IGH and IGK gene rearrangements were mainly found in mantle cell lymphomas, small lymphocytic lymphomas, extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue and diffuse large B-cell lymphomas. The IGH gene rearrangement was mainly found in lymphoplasmacytoid lymphomas and follicular lymphomas. IGK and IGL gene rearrangements were mainly found in plasmocytoma, and the IGK gene rearrangement was mainly found in nodal marginal zone B-cell lymphomas. CONCLUSIONS: The BIOMED-2 standardized immunoglobulin gene rearrangement detection system is an important tool in B-cell lymphoma diagnosis. Analysis of IGH, IGK and IGL gene rearrangements is valuable in confirming the classification of B-cell NHL. Impact Journals LLC 2017-08-18 /pmc/articles/PMC5652759/ /pubmed/29100365 http://dx.doi.org/10.18632/oncotarget.20330 Text en Copyright: © 2017 Lu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Lu, Can
He, QiuYan
Zhu, Wei
Fu, ChunYan
Zhou, JianHua
Tao, YongGuang
Liu, Shuang
Xiao, DeSheng
The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma
title The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma
title_full The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma
title_fullStr The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma
title_full_unstemmed The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma
title_short The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma
title_sort value of detecting immunoglobulin gene rearrangements in the diagnosis of b-cell lymphoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652759/
https://www.ncbi.nlm.nih.gov/pubmed/29100365
http://dx.doi.org/10.18632/oncotarget.20330
work_keys_str_mv AT lucan thevalueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT heqiuyan thevalueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT zhuwei thevalueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT fuchunyan thevalueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT zhoujianhua thevalueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT taoyongguang thevalueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT liushuang thevalueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT xiaodesheng thevalueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT lucan valueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT heqiuyan valueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT zhuwei valueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT fuchunyan valueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT zhoujianhua valueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT taoyongguang valueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT liushuang valueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma
AT xiaodesheng valueofdetectingimmunoglobulingenerearrangementsinthediagnosisofbcelllymphoma