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The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma
OBJECTIVE: To discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma. METHODS: A total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652759/ https://www.ncbi.nlm.nih.gov/pubmed/29100365 http://dx.doi.org/10.18632/oncotarget.20330 |
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author | Lu, Can He, QiuYan Zhu, Wei Fu, ChunYan Zhou, JianHua Tao, YongGuang Liu, Shuang Xiao, DeSheng |
author_facet | Lu, Can He, QiuYan Zhu, Wei Fu, ChunYan Zhou, JianHua Tao, YongGuang Liu, Shuang Xiao, DeSheng |
author_sort | Lu, Can |
collection | PubMed |
description | OBJECTIVE: To discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma. METHODS: A total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system. Gel electrophoresis of heteroduplexes was used to analyze immunoglobulin gene rearrangements. RESULTS: A total of 209 cases of B-cell lymphoma, including 69 extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue, 63 diffuse large B-cell lymphomas, 39 follicular lymphomas, 15 small lymphocytic lymphomas, 6 plasmacytomas, 6 mantle cell lymphomas, 7 nodal marginal zone B-cell lymphomas, and 4 lymphoplasmacytoid lymphomas, were examined. Immunoglobulin gene rearrangements were found in all 209 cases, with 93 IGHA, 122 IGHB, 98 IGHC, 167 IGK, 100 IGL, 167 IGHA/B/C, 204 IGH/IGK, 209 IGH/IGK/IGL, 129 IGH+IGK, 81 IGH+IGL, 83 IGK+IGL and 68 IGH+IGK+IGL gene rearrangements. Immunoglobulin gene rearrangements were not found in the 35 cases of reactive lymphoid hyperplasia. IGH and IGK gene rearrangements were mainly found in mantle cell lymphomas, small lymphocytic lymphomas, extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue and diffuse large B-cell lymphomas. The IGH gene rearrangement was mainly found in lymphoplasmacytoid lymphomas and follicular lymphomas. IGK and IGL gene rearrangements were mainly found in plasmocytoma, and the IGK gene rearrangement was mainly found in nodal marginal zone B-cell lymphomas. CONCLUSIONS: The BIOMED-2 standardized immunoglobulin gene rearrangement detection system is an important tool in B-cell lymphoma diagnosis. Analysis of IGH, IGK and IGL gene rearrangements is valuable in confirming the classification of B-cell NHL. |
format | Online Article Text |
id | pubmed-5652759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56527592017-11-02 The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma Lu, Can He, QiuYan Zhu, Wei Fu, ChunYan Zhou, JianHua Tao, YongGuang Liu, Shuang Xiao, DeSheng Oncotarget Research Paper OBJECTIVE: To discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma. METHODS: A total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system. Gel electrophoresis of heteroduplexes was used to analyze immunoglobulin gene rearrangements. RESULTS: A total of 209 cases of B-cell lymphoma, including 69 extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue, 63 diffuse large B-cell lymphomas, 39 follicular lymphomas, 15 small lymphocytic lymphomas, 6 plasmacytomas, 6 mantle cell lymphomas, 7 nodal marginal zone B-cell lymphomas, and 4 lymphoplasmacytoid lymphomas, were examined. Immunoglobulin gene rearrangements were found in all 209 cases, with 93 IGHA, 122 IGHB, 98 IGHC, 167 IGK, 100 IGL, 167 IGHA/B/C, 204 IGH/IGK, 209 IGH/IGK/IGL, 129 IGH+IGK, 81 IGH+IGL, 83 IGK+IGL and 68 IGH+IGK+IGL gene rearrangements. Immunoglobulin gene rearrangements were not found in the 35 cases of reactive lymphoid hyperplasia. IGH and IGK gene rearrangements were mainly found in mantle cell lymphomas, small lymphocytic lymphomas, extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue and diffuse large B-cell lymphomas. The IGH gene rearrangement was mainly found in lymphoplasmacytoid lymphomas and follicular lymphomas. IGK and IGL gene rearrangements were mainly found in plasmocytoma, and the IGK gene rearrangement was mainly found in nodal marginal zone B-cell lymphomas. CONCLUSIONS: The BIOMED-2 standardized immunoglobulin gene rearrangement detection system is an important tool in B-cell lymphoma diagnosis. Analysis of IGH, IGK and IGL gene rearrangements is valuable in confirming the classification of B-cell NHL. Impact Journals LLC 2017-08-18 /pmc/articles/PMC5652759/ /pubmed/29100365 http://dx.doi.org/10.18632/oncotarget.20330 Text en Copyright: © 2017 Lu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Lu, Can He, QiuYan Zhu, Wei Fu, ChunYan Zhou, JianHua Tao, YongGuang Liu, Shuang Xiao, DeSheng The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma |
title | The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma |
title_full | The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma |
title_fullStr | The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma |
title_full_unstemmed | The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma |
title_short | The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma |
title_sort | value of detecting immunoglobulin gene rearrangements in the diagnosis of b-cell lymphoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652759/ https://www.ncbi.nlm.nih.gov/pubmed/29100365 http://dx.doi.org/10.18632/oncotarget.20330 |
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