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Upregulated exosomic miR-23b-3p plays regulatory roles in the progression of pancreatic cancer

Pancreatic cancer (PC) is one of the most lethal malignances. Identification of biomarkers for early diagnosis of PC is a key imperative. MicroRNAs (miRNAs) have been shown to be valuable biomarkers in the context of several cancers. Exosomes refer to vesicles released by the tumor cells at the earl...

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Autores principales: Chen, Dayang, Wu, Xiongbo, Xia, Min, Wu, Fang, Ding, Junli, Jiao, Yang, Zhan, Qiang, An, Fangmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652966/
https://www.ncbi.nlm.nih.gov/pubmed/28849236
http://dx.doi.org/10.3892/or.2017.5919
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author Chen, Dayang
Wu, Xiongbo
Xia, Min
Wu, Fang
Ding, Junli
Jiao, Yang
Zhan, Qiang
An, Fangmei
author_facet Chen, Dayang
Wu, Xiongbo
Xia, Min
Wu, Fang
Ding, Junli
Jiao, Yang
Zhan, Qiang
An, Fangmei
author_sort Chen, Dayang
collection PubMed
description Pancreatic cancer (PC) is one of the most lethal malignances. Identification of biomarkers for early diagnosis of PC is a key imperative. MicroRNAs (miRNAs) have been shown to be valuable biomarkers in the context of several cancers. Exosomes refer to vesicles released by the tumor cells at the early stage of disease. Thus, detection of miRNA in exosomes can be used as a potential biomarker for PC. In this study, we profiled serum levels of miRNAs in patients with chronic pancreatitis (CP) and PC; the role of miR-23b-3p in PC progression was assessed in vitro. Additionally, we assessed, the expression of miR-23b-3p in exosomes isolated from serum samples and assessed the correlation between the expression of miR-23b-3p and carbohydrate antigen 19-9 (CA19-9). Three serum samples each were randomly selected from healthy controls (n=20), and patients with CP (n=18) and PC (n=16) for miRNA microarray profiling. The dysregulated miRNAs were confirmed using qRT-PCR. Four dysregulated miRNAs common to patients with CP and PC were identified on miRNA microarray analysis and confirmed by qRT-PCR. miR-23b-3p level was consistently higher in serum samples from PC patients as compared to those from healthy controls and CP patients (p<0.05). Overexpression of miR-23b-3p promoted proliferation, migration, and invasion capability of PC cells in vitro (p<0.05). Furthermore, miR-23b-3p was upregulated in exosomes of PC serum samples and the supernatant of pancreatic cancer cells (PANC-1), and the expression levels of miR-23b-3p were associated with those of serum CA19-9 levels. This study provides insights into the potential role of miR-23b-3p as a novel biomarker and target for treatment of PC.
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spelling pubmed-56529662017-11-02 Upregulated exosomic miR-23b-3p plays regulatory roles in the progression of pancreatic cancer Chen, Dayang Wu, Xiongbo Xia, Min Wu, Fang Ding, Junli Jiao, Yang Zhan, Qiang An, Fangmei Oncol Rep Articles Pancreatic cancer (PC) is one of the most lethal malignances. Identification of biomarkers for early diagnosis of PC is a key imperative. MicroRNAs (miRNAs) have been shown to be valuable biomarkers in the context of several cancers. Exosomes refer to vesicles released by the tumor cells at the early stage of disease. Thus, detection of miRNA in exosomes can be used as a potential biomarker for PC. In this study, we profiled serum levels of miRNAs in patients with chronic pancreatitis (CP) and PC; the role of miR-23b-3p in PC progression was assessed in vitro. Additionally, we assessed, the expression of miR-23b-3p in exosomes isolated from serum samples and assessed the correlation between the expression of miR-23b-3p and carbohydrate antigen 19-9 (CA19-9). Three serum samples each were randomly selected from healthy controls (n=20), and patients with CP (n=18) and PC (n=16) for miRNA microarray profiling. The dysregulated miRNAs were confirmed using qRT-PCR. Four dysregulated miRNAs common to patients with CP and PC were identified on miRNA microarray analysis and confirmed by qRT-PCR. miR-23b-3p level was consistently higher in serum samples from PC patients as compared to those from healthy controls and CP patients (p<0.05). Overexpression of miR-23b-3p promoted proliferation, migration, and invasion capability of PC cells in vitro (p<0.05). Furthermore, miR-23b-3p was upregulated in exosomes of PC serum samples and the supernatant of pancreatic cancer cells (PANC-1), and the expression levels of miR-23b-3p were associated with those of serum CA19-9 levels. This study provides insights into the potential role of miR-23b-3p as a novel biomarker and target for treatment of PC. D.A. Spandidos 2017-10 2017-08-24 /pmc/articles/PMC5652966/ /pubmed/28849236 http://dx.doi.org/10.3892/or.2017.5919 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Chen, Dayang
Wu, Xiongbo
Xia, Min
Wu, Fang
Ding, Junli
Jiao, Yang
Zhan, Qiang
An, Fangmei
Upregulated exosomic miR-23b-3p plays regulatory roles in the progression of pancreatic cancer
title Upregulated exosomic miR-23b-3p plays regulatory roles in the progression of pancreatic cancer
title_full Upregulated exosomic miR-23b-3p plays regulatory roles in the progression of pancreatic cancer
title_fullStr Upregulated exosomic miR-23b-3p plays regulatory roles in the progression of pancreatic cancer
title_full_unstemmed Upregulated exosomic miR-23b-3p plays regulatory roles in the progression of pancreatic cancer
title_short Upregulated exosomic miR-23b-3p plays regulatory roles in the progression of pancreatic cancer
title_sort upregulated exosomic mir-23b-3p plays regulatory roles in the progression of pancreatic cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652966/
https://www.ncbi.nlm.nih.gov/pubmed/28849236
http://dx.doi.org/10.3892/or.2017.5919
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