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Prohibition of antibiotic growth promoters has affected the genomic profiles of Lactobacillus salivarius inhabiting the swine intestine

After the introduction of a ban on the use of antibiotic growth promoters (AGPs) for livestock, the feeding environment, including the composition of animal intestinal microbiota, has changed rapidly. We hypothesized that the microbial genomes have also been affected by this legal prohibition, and i...

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Autores principales: Lee, Jun-Yeong, Han, Geon Goo, Lee, Ho-Bin, Lee, Sang-Mok, Kang, Sang-Kee, Jin, Gwi-Deuk, Park, Jongbin, Chae, Byung Jo, Choi, Yo Han, Kim, Eun Bae, Choi, Yun-Jaie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653324/
https://www.ncbi.nlm.nih.gov/pubmed/29059217
http://dx.doi.org/10.1371/journal.pone.0186671
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author Lee, Jun-Yeong
Han, Geon Goo
Lee, Ho-Bin
Lee, Sang-Mok
Kang, Sang-Kee
Jin, Gwi-Deuk
Park, Jongbin
Chae, Byung Jo
Choi, Yo Han
Kim, Eun Bae
Choi, Yun-Jaie
author_facet Lee, Jun-Yeong
Han, Geon Goo
Lee, Ho-Bin
Lee, Sang-Mok
Kang, Sang-Kee
Jin, Gwi-Deuk
Park, Jongbin
Chae, Byung Jo
Choi, Yo Han
Kim, Eun Bae
Choi, Yun-Jaie
author_sort Lee, Jun-Yeong
collection PubMed
description After the introduction of a ban on the use of antibiotic growth promoters (AGPs) for livestock, the feeding environment, including the composition of animal intestinal microbiota, has changed rapidly. We hypothesized that the microbial genomes have also been affected by this legal prohibition, and investigated an important member of the swine gut microbiota, Lactobacillus salivarius, with a pan-genomic approach. Here, we isolated 21 L. salivarius strains composed of 6 strains isolated before the AGP prohibition (SBPs) and 15 strains isolated after the AGP prohibition (SAPs) at an interval of a decade, and the draft genomes were generated de novo. Several genomic differences between SBPs and SAPs were identified, although the number and function of antibiotic resistance genes were not different. SBPs showed larger genome size and a higher number of orthologs, as well as lower genetic diversity, than SAPs. SBPs had genes associated with the utilization of L-rhamnose and D-tagatose for energy production. Because these sugars are also used in exopolysaccharide (EPS) synthesis, we tried to identify differences in biofilm formation-associated genes. The genes for the production of EPSs and extracellular proteins were different in terms of amino acid sequences. Indeed, SAPs formed dense biofilm and survived better than SBPs in the swine intestinal environment. These results suggest that SAPs have evolved and adapted to protect themselves from new selection pressure of the swine intestinal microenvironment by forming dense biofilms, adopting a distinct antibiotic resistance strategy. This finding is particularly important to understand the evolutionary changes in host-microbe interaction and provide detailed insight for the development of effective probiotics for livestock.
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spelling pubmed-56533242017-11-08 Prohibition of antibiotic growth promoters has affected the genomic profiles of Lactobacillus salivarius inhabiting the swine intestine Lee, Jun-Yeong Han, Geon Goo Lee, Ho-Bin Lee, Sang-Mok Kang, Sang-Kee Jin, Gwi-Deuk Park, Jongbin Chae, Byung Jo Choi, Yo Han Kim, Eun Bae Choi, Yun-Jaie PLoS One Research Article After the introduction of a ban on the use of antibiotic growth promoters (AGPs) for livestock, the feeding environment, including the composition of animal intestinal microbiota, has changed rapidly. We hypothesized that the microbial genomes have also been affected by this legal prohibition, and investigated an important member of the swine gut microbiota, Lactobacillus salivarius, with a pan-genomic approach. Here, we isolated 21 L. salivarius strains composed of 6 strains isolated before the AGP prohibition (SBPs) and 15 strains isolated after the AGP prohibition (SAPs) at an interval of a decade, and the draft genomes were generated de novo. Several genomic differences between SBPs and SAPs were identified, although the number and function of antibiotic resistance genes were not different. SBPs showed larger genome size and a higher number of orthologs, as well as lower genetic diversity, than SAPs. SBPs had genes associated with the utilization of L-rhamnose and D-tagatose for energy production. Because these sugars are also used in exopolysaccharide (EPS) synthesis, we tried to identify differences in biofilm formation-associated genes. The genes for the production of EPSs and extracellular proteins were different in terms of amino acid sequences. Indeed, SAPs formed dense biofilm and survived better than SBPs in the swine intestinal environment. These results suggest that SAPs have evolved and adapted to protect themselves from new selection pressure of the swine intestinal microenvironment by forming dense biofilms, adopting a distinct antibiotic resistance strategy. This finding is particularly important to understand the evolutionary changes in host-microbe interaction and provide detailed insight for the development of effective probiotics for livestock. Public Library of Science 2017-10-23 /pmc/articles/PMC5653324/ /pubmed/29059217 http://dx.doi.org/10.1371/journal.pone.0186671 Text en © 2017 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lee, Jun-Yeong
Han, Geon Goo
Lee, Ho-Bin
Lee, Sang-Mok
Kang, Sang-Kee
Jin, Gwi-Deuk
Park, Jongbin
Chae, Byung Jo
Choi, Yo Han
Kim, Eun Bae
Choi, Yun-Jaie
Prohibition of antibiotic growth promoters has affected the genomic profiles of Lactobacillus salivarius inhabiting the swine intestine
title Prohibition of antibiotic growth promoters has affected the genomic profiles of Lactobacillus salivarius inhabiting the swine intestine
title_full Prohibition of antibiotic growth promoters has affected the genomic profiles of Lactobacillus salivarius inhabiting the swine intestine
title_fullStr Prohibition of antibiotic growth promoters has affected the genomic profiles of Lactobacillus salivarius inhabiting the swine intestine
title_full_unstemmed Prohibition of antibiotic growth promoters has affected the genomic profiles of Lactobacillus salivarius inhabiting the swine intestine
title_short Prohibition of antibiotic growth promoters has affected the genomic profiles of Lactobacillus salivarius inhabiting the swine intestine
title_sort prohibition of antibiotic growth promoters has affected the genomic profiles of lactobacillus salivarius inhabiting the swine intestine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653324/
https://www.ncbi.nlm.nih.gov/pubmed/29059217
http://dx.doi.org/10.1371/journal.pone.0186671
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