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Formation of the chaperonin complex studied by 2D NMR spectroscopy
We studied the interaction between GroES and a single-ring mutant (SR1) of GroEL by the NMR titration of (15)N-labeled GroES with SR1 at three different temperatures (20, 25 and 30°C) in the presence of 3 mM ADP in 100 mM KCl and 10 mM MgCl(2) at pH 7.5. We used SR1 instead of wild-type double-ring...
Autores principales: | Takenaka, Toshio, Nakamura, Takashi, Yanaka, Saeko, Yagi-Utsumi, Maho, Chandak, Mahesh S., Takahashi, Kazunobu, Paul, Subhankar, Makabe, Koki, Arai, Munehito, Kato, Koichi, Kuwajima, Kunihiro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653362/ https://www.ncbi.nlm.nih.gov/pubmed/29059240 http://dx.doi.org/10.1371/journal.pone.0187022 |
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