Cargando…
Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty
BACKGROUND: common use of sevoflurane in congenital defects during repeated surgeries may have detrimental effects on spermatogenesis after puberty. OBJECTIVE: This study investigated sevoflurane effects on spermatogenesis process in male mature mice after exposure in prepubertal time. MATERIALS AND...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research and Clinical Center for Infertility
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653908/ https://www.ncbi.nlm.nih.gov/pubmed/29082365 |
_version_ | 1783273305129615360 |
---|---|
author | Nezhad Sistani, Maryam Maleki, Anahid Salimi, Maryam Ghaffari Novin, Marefat Nazarian, Hamid |
author_facet | Nezhad Sistani, Maryam Maleki, Anahid Salimi, Maryam Ghaffari Novin, Marefat Nazarian, Hamid |
author_sort | Nezhad Sistani, Maryam |
collection | PubMed |
description | BACKGROUND: common use of sevoflurane in congenital defects during repeated surgeries may have detrimental effects on spermatogenesis after puberty. OBJECTIVE: This study investigated sevoflurane effects on spermatogenesis process in male mature mice after exposure in prepubertal time. MATERIALS AND METHODS: 24 neonatal NMRI male mice were randomly classified in three groups. Experimental 1 and 2 groups (exposure to 1 minimum alveolar concentration (MAC) and 2 MAC sevoflurane, respectively in 2 lit/min oxygen (O(2)) for 7 days (30 min, daily) and control. All groups were sacrificed after 2 months. Histological assessment, immunohistochemistry and apoptosis process was done. Bax and Bcl(2) expression was evaluated in the testicular tissue by real time Poly Chain Reaction. RESULTS: Our results showed that the integrity of testicular tissue was preserved in both experimental groups. Count of spermatogonial cells had significant decrease in group 2 compared to others. The rate of apoptosis in spermatogonial cells was 15±3% and 9±2% in the group 2 and 1, respectively. Also, Bax/Bcl(2) ratio was 0.2615, 1.0070 and 9.3657 in control, experimental group 1 and 2, respectively. This result was significant (p≤0.002) between groups 2 with other groups. CONCLUSION: Continuous exposure of 2 MAC sevoflurane in 2 lit/min O(2) simultaneous during prepubertal may create more testicular tissue damage in terms of cellular and molecular function compared to continuous exposure to lower level of sevoflurane by increase in ratio of Bax/Bcl(2) and apoptosis in germ cells after puberty. |
format | Online Article Text |
id | pubmed-5653908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Research and Clinical Center for Infertility |
record_format | MEDLINE/PubMed |
spelling | pubmed-56539082017-10-27 Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty Nezhad Sistani, Maryam Maleki, Anahid Salimi, Maryam Ghaffari Novin, Marefat Nazarian, Hamid Int J Reprod Biomed Original Article BACKGROUND: common use of sevoflurane in congenital defects during repeated surgeries may have detrimental effects on spermatogenesis after puberty. OBJECTIVE: This study investigated sevoflurane effects on spermatogenesis process in male mature mice after exposure in prepubertal time. MATERIALS AND METHODS: 24 neonatal NMRI male mice were randomly classified in three groups. Experimental 1 and 2 groups (exposure to 1 minimum alveolar concentration (MAC) and 2 MAC sevoflurane, respectively in 2 lit/min oxygen (O(2)) for 7 days (30 min, daily) and control. All groups were sacrificed after 2 months. Histological assessment, immunohistochemistry and apoptosis process was done. Bax and Bcl(2) expression was evaluated in the testicular tissue by real time Poly Chain Reaction. RESULTS: Our results showed that the integrity of testicular tissue was preserved in both experimental groups. Count of spermatogonial cells had significant decrease in group 2 compared to others. The rate of apoptosis in spermatogonial cells was 15±3% and 9±2% in the group 2 and 1, respectively. Also, Bax/Bcl(2) ratio was 0.2615, 1.0070 and 9.3657 in control, experimental group 1 and 2, respectively. This result was significant (p≤0.002) between groups 2 with other groups. CONCLUSION: Continuous exposure of 2 MAC sevoflurane in 2 lit/min O(2) simultaneous during prepubertal may create more testicular tissue damage in terms of cellular and molecular function compared to continuous exposure to lower level of sevoflurane by increase in ratio of Bax/Bcl(2) and apoptosis in germ cells after puberty. Research and Clinical Center for Infertility 2017-08 /pmc/articles/PMC5653908/ /pubmed/29082365 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nezhad Sistani, Maryam Maleki, Anahid Salimi, Maryam Ghaffari Novin, Marefat Nazarian, Hamid Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty |
title | Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty |
title_full | Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty |
title_fullStr | Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty |
title_full_unstemmed | Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty |
title_short | Exposure of neonatal mice to Sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty |
title_sort | exposure of neonatal mice to sevoflurane may induce male germ cell apoptosis in testicular tissue after puberty |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653908/ https://www.ncbi.nlm.nih.gov/pubmed/29082365 |
work_keys_str_mv | AT nezhadsistanimaryam exposureofneonatalmicetosevofluranemayinducemalegermcellapoptosisintesticulartissueafterpuberty AT malekianahid exposureofneonatalmicetosevofluranemayinducemalegermcellapoptosisintesticulartissueafterpuberty AT salimimaryam exposureofneonatalmicetosevofluranemayinducemalegermcellapoptosisintesticulartissueafterpuberty AT ghaffarinovinmarefat exposureofneonatalmicetosevofluranemayinducemalegermcellapoptosisintesticulartissueafterpuberty AT nazarianhamid exposureofneonatalmicetosevofluranemayinducemalegermcellapoptosisintesticulartissueafterpuberty |