Ionic Strength Sensing in Living Cells

[Image: see text] Knowledge of the ionic strength in cells is required to understand the in vivo biochemistry of the charged biomacromolecules. Here, we present the first sensors to determine the ionic strength in living cells, by designing protein probes based on Förster resonance energy transfer (...

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Detalles Bibliográficos
Autores principales: Liu, Boqun, Poolman, Bert, Boersma, Arnold J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653947/
https://www.ncbi.nlm.nih.gov/pubmed/28853549
http://dx.doi.org/10.1021/acschembio.7b00348
Descripción
Sumario:[Image: see text] Knowledge of the ionic strength in cells is required to understand the in vivo biochemistry of the charged biomacromolecules. Here, we present the first sensors to determine the ionic strength in living cells, by designing protein probes based on Förster resonance energy transfer (FRET). These probes allow observation of spatiotemporal changes in the ionic strength on the single-cell level.

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