Ionic Strength Sensing in Living Cells

[Image: see text] Knowledge of the ionic strength in cells is required to understand the in vivo biochemistry of the charged biomacromolecules. Here, we present the first sensors to determine the ionic strength in living cells, by designing protein probes based on Förster resonance energy transfer (...

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Detalles Bibliográficos
Autores principales: Liu, Boqun, Poolman, Bert, Boersma, Arnold J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653947/
https://www.ncbi.nlm.nih.gov/pubmed/28853549
http://dx.doi.org/10.1021/acschembio.7b00348
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author Liu, Boqun
Poolman, Bert
Boersma, Arnold J.
author_facet Liu, Boqun
Poolman, Bert
Boersma, Arnold J.
author_sort Liu, Boqun
collection PubMed
description [Image: see text] Knowledge of the ionic strength in cells is required to understand the in vivo biochemistry of the charged biomacromolecules. Here, we present the first sensors to determine the ionic strength in living cells, by designing protein probes based on Förster resonance energy transfer (FRET). These probes allow observation of spatiotemporal changes in the ionic strength on the single-cell level.
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spelling pubmed-56539472017-10-26 Ionic Strength Sensing in Living Cells Liu, Boqun Poolman, Bert Boersma, Arnold J. ACS Chem Biol [Image: see text] Knowledge of the ionic strength in cells is required to understand the in vivo biochemistry of the charged biomacromolecules. Here, we present the first sensors to determine the ionic strength in living cells, by designing protein probes based on Förster resonance energy transfer (FRET). These probes allow observation of spatiotemporal changes in the ionic strength on the single-cell level. American Chemical Society 2017-08-30 2017-10-20 /pmc/articles/PMC5653947/ /pubmed/28853549 http://dx.doi.org/10.1021/acschembio.7b00348 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Liu, Boqun
Poolman, Bert
Boersma, Arnold J.
Ionic Strength Sensing in Living Cells
title Ionic Strength Sensing in Living Cells
title_full Ionic Strength Sensing in Living Cells
title_fullStr Ionic Strength Sensing in Living Cells
title_full_unstemmed Ionic Strength Sensing in Living Cells
title_short Ionic Strength Sensing in Living Cells
title_sort ionic strength sensing in living cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653947/
https://www.ncbi.nlm.nih.gov/pubmed/28853549
http://dx.doi.org/10.1021/acschembio.7b00348
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