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Comparative cytogenetics in three Sciaenid species (Teleostei, Perciformes): evidence of interspecific chromosomal diversification

BACKGROUND: Species belonging to the Sciaenidae family present a karyotype composed by 48 acrocentric chromosomes and are thus considered a striking example of chromosomal conservation. In this family, three species are extensively studied including Larimichthys crocea, Larimichthys polyactis and Ni...

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Autores principales: Xu, Dongdong, Molina, Wagner Franco, Yano, Cassia Fernanda, Zhang, Yurong, de Oliveira, Ezequiel Aguiar, Lou, Bao, de Bello Cioffi, Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5654061/
https://www.ncbi.nlm.nih.gov/pubmed/29075328
http://dx.doi.org/10.1186/s13039-017-0338-0
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author Xu, Dongdong
Molina, Wagner Franco
Yano, Cassia Fernanda
Zhang, Yurong
de Oliveira, Ezequiel Aguiar
Lou, Bao
de Bello Cioffi, Marcelo
author_facet Xu, Dongdong
Molina, Wagner Franco
Yano, Cassia Fernanda
Zhang, Yurong
de Oliveira, Ezequiel Aguiar
Lou, Bao
de Bello Cioffi, Marcelo
author_sort Xu, Dongdong
collection PubMed
description BACKGROUND: Species belonging to the Sciaenidae family present a karyotype composed by 48 acrocentric chromosomes and are thus considered a striking example of chromosomal conservation. In this family, three species are extensively studied including Larimichthys crocea, Larimichthys polyactis and Nibea albiflora due to their importance in fishery and aquaculture in East Asia. Despite abundant data of population genetics available for some of them, cytogenetic information on these species is still scarce and obtained by conventional cytogenetic protocols. Therefore, a more detailed cytogenomic investigation was performed in these species to analyze their karyotype differentiation using conventional staining techniques and fluorescence in situ hybridization to map several repetitive DNAs. RESULTS: The three species showed a slight karyotype differentiation with 4sm + 2st + 42a in L. polyactis, 20st + 28a in L. crocea and 48a in N. albiflora. Additionally, the mapping of repetitive sequences further revealed a number of interspecific differences among them. Particularly, 18S and 5S rDNA sites showed syntenic arrangements in N. albiflora and non-syntenic arrangements in both Larimichthys species. The microsatellites (CA)(15) and (GA)(15) showed conspicuous terminal clusters in some chromosomes of all species. On the other hand, (CGG)(10) repeats, Rex6 elements and U2 snRNA displayed a scattered distribution on the chromosomes. CONCLUSIONS: Although the three Sciaenid species examined displayed a general pattern of karyotypic conservatism, we explored chromosomal diversification among them. The diversificated karyotypic macrostructure is followed by intergeneric evolutionary diversification of the repetitive sequences. The data indicate some degree of intergeneric evolutionary diversification at chromosomal level, and suggest the evolutionary dynamics among Sciaenid species, higher than previously thought. The present cytogenetic data provide new insight into the chromosomal diversification in Sciaenidae, and contribute to inferring the chromosomal rearrangements and trends of karyotype evolution in this fish group.
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spelling pubmed-56540612017-10-26 Comparative cytogenetics in three Sciaenid species (Teleostei, Perciformes): evidence of interspecific chromosomal diversification Xu, Dongdong Molina, Wagner Franco Yano, Cassia Fernanda Zhang, Yurong de Oliveira, Ezequiel Aguiar Lou, Bao de Bello Cioffi, Marcelo Mol Cytogenet Research BACKGROUND: Species belonging to the Sciaenidae family present a karyotype composed by 48 acrocentric chromosomes and are thus considered a striking example of chromosomal conservation. In this family, three species are extensively studied including Larimichthys crocea, Larimichthys polyactis and Nibea albiflora due to their importance in fishery and aquaculture in East Asia. Despite abundant data of population genetics available for some of them, cytogenetic information on these species is still scarce and obtained by conventional cytogenetic protocols. Therefore, a more detailed cytogenomic investigation was performed in these species to analyze their karyotype differentiation using conventional staining techniques and fluorescence in situ hybridization to map several repetitive DNAs. RESULTS: The three species showed a slight karyotype differentiation with 4sm + 2st + 42a in L. polyactis, 20st + 28a in L. crocea and 48a in N. albiflora. Additionally, the mapping of repetitive sequences further revealed a number of interspecific differences among them. Particularly, 18S and 5S rDNA sites showed syntenic arrangements in N. albiflora and non-syntenic arrangements in both Larimichthys species. The microsatellites (CA)(15) and (GA)(15) showed conspicuous terminal clusters in some chromosomes of all species. On the other hand, (CGG)(10) repeats, Rex6 elements and U2 snRNA displayed a scattered distribution on the chromosomes. CONCLUSIONS: Although the three Sciaenid species examined displayed a general pattern of karyotypic conservatism, we explored chromosomal diversification among them. The diversificated karyotypic macrostructure is followed by intergeneric evolutionary diversification of the repetitive sequences. The data indicate some degree of intergeneric evolutionary diversification at chromosomal level, and suggest the evolutionary dynamics among Sciaenid species, higher than previously thought. The present cytogenetic data provide new insight into the chromosomal diversification in Sciaenidae, and contribute to inferring the chromosomal rearrangements and trends of karyotype evolution in this fish group. BioMed Central 2017-10-23 /pmc/articles/PMC5654061/ /pubmed/29075328 http://dx.doi.org/10.1186/s13039-017-0338-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xu, Dongdong
Molina, Wagner Franco
Yano, Cassia Fernanda
Zhang, Yurong
de Oliveira, Ezequiel Aguiar
Lou, Bao
de Bello Cioffi, Marcelo
Comparative cytogenetics in three Sciaenid species (Teleostei, Perciformes): evidence of interspecific chromosomal diversification
title Comparative cytogenetics in three Sciaenid species (Teleostei, Perciformes): evidence of interspecific chromosomal diversification
title_full Comparative cytogenetics in three Sciaenid species (Teleostei, Perciformes): evidence of interspecific chromosomal diversification
title_fullStr Comparative cytogenetics in three Sciaenid species (Teleostei, Perciformes): evidence of interspecific chromosomal diversification
title_full_unstemmed Comparative cytogenetics in three Sciaenid species (Teleostei, Perciformes): evidence of interspecific chromosomal diversification
title_short Comparative cytogenetics in three Sciaenid species (Teleostei, Perciformes): evidence of interspecific chromosomal diversification
title_sort comparative cytogenetics in three sciaenid species (teleostei, perciformes): evidence of interspecific chromosomal diversification
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5654061/
https://www.ncbi.nlm.nih.gov/pubmed/29075328
http://dx.doi.org/10.1186/s13039-017-0338-0
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