Cargando…

Use of a bioinformatic-assisted primer design strategy to establish a new nested PCR-based method for Cryptosporidium

BACKGROUND: The accurate tracking of Cryptosporidium in faecal, water and/or soil samples in water catchment areas is central to developing strategies to manage the potential risk of cryptosporidiosis transmission to humans. Various PCR assays are used for this purpose. Although some assays achieve...

Descripción completa

Detalles Bibliográficos
Autores principales:	Koehler, Anson V., Korhonen, Pasi K., Hall, Ross S., Young, Neil D., Wang, Tao, Haydon, Shane R., Gasser, Robin B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5654123/ https://www.ncbi.nlm.nih.gov/pubmed/29061171 http://dx.doi.org/10.1186/s13071-017-2462-4

Ejemplares similares

Cryptosporidium cuniculus - new records in human and kangaroo in Australia
por: Koehler, Anson V, et al.
Publicado: (2014)

Cryptosporidium viatorum from the native Australian swamp rat Rattus lutreolus - An emerging zoonotic pathogen?
por: Koehler, Anson V., et al.
Publicado: (2018)

Cryptosporidium and Giardia taxa in faecal samples from animals in catchments supplying the city of Melbourne with drinking water (2011 to 2015)
por: Koehler, Anson V., et al.
Publicado: (2016)

Cryptosporidium cf. avium in an inland-bearded dragon (Pogona vitticeps) – A case report and review of the literature
por: Koehler, Anson V., et al.
Publicado: (2020)

Cryptosporidium of birds in pet markets in Wuhan city, Hubei, China
por: Liao, Cong, et al.
Publicado: (2021)

First molecular characterization of Cryptosporidium and Giardia from bovines (Bos taurus and Bubalus bubalis) in Sri Lanka: unexpected absence of C. parvum from pre-weaned calves
por: Abeywardena, Harshanie, et al.
Publicado: (2014)

Molecular investigation of Cryptosporidium and Giardia in pre- and post-weaned calves in Hubei Province, China
por: Fan, Yingying, et al.
Publicado: (2017)

First detection and genetic characterisation of Enterocytozoon bieneusi in wild deer in Melbourne’s water catchments in Australia
por: Zhang, Yan, et al.
Publicado: (2018)

New operational taxonomic units of Enterocytozoon in three marsupial species
por: Zhang, Yan, et al.
Publicado: (2018)

Multiplex PCRs for the specific identification of marsupial and deer species from faecal samples as a basis for non-invasive epidemiological studies of parasites
por: Koehler, Anson V., et al.
Publicado: (2020)

Molecular investigation of Cryptosporidium in farmed chickens in Hubei Province, China, identifies ‘zoonotic’ subtypes of C. meleagridis
por: Liao, Cong, et al.
Publicado: (2018)

Common workflow language (CWL)-based software pipeline for de novo genome assembly from long- and short-read data
por: Korhonen, Pasi K, et al.
Publicado: (2019)

Targeted Next-Generation Sequencing and Informatics as an Effective Tool to Establish the Composition of Bovine Piroplasm Populations in Endemic Regions
por: Ghafar, Abdul, et al.
Publicado: (2020)

Comparative bioinformatic analysis suggests that specific dauer-like signalling pathway components regulate Toxocara canis development and migration in the mammalian host
por: Ma, Guangxu, et al.
Publicado: (2019)

First cross-sectional, molecular epidemiological survey of Cryptosporidium, Giardia and Enterocytozoon in alpaca (Vicugna pacos) in Australia
por: Koehler, Anson V., et al.
Publicado: (2018)

A Targeted “Next-Generation” Sequencing-Informatic Approach to Define Genetic Diversity in Theileria orientalis Populations within Individual Cattle: Proof-of-Principle
por: Koehler, Anson V., et al.
Publicado: (2020)

Using PCR-Based Sequencing to Diagnose Haycocknema perplexum Infection in Human Myositis Case, Australia
por: Koehler, Anson V., et al.
Publicado: (2018)

Genomic resources for a unique, low-virulence Babesia taxon from China
por: Guan, Guiquan, et al.
Publicado: (2016)

Marked mitochondrial genetic variation in individuals and populations of the carcinogenic liver fluke Clonorchis sinensis
por: Kinkar, Liina, et al.
Publicado: (2020)

Pipeline for the identification and classification of ion channels in parasitic flatworms
por: Nor, Bahiyah, et al.
Publicado: (2016)

A Bioinformatics Approach to Identifying Potential Biomarkers for Cryptosporidium parvum: A Coccidian Parasite Associated with Fetal Diarrhea
por: Sabir, Mumdooh J., et al.
Publicado: (2021)

SpeciesPrimer: a bioinformatics pipeline dedicated to the design of qPCR primers for the quantification of bacterial species
por: Dreier, Matthias, et al.
Publicado: (2020)

Application of PCR-Based Tools to Explore Strongyloides Infection in People in Parts of Northern Australia
por: Robertson, Gemma J., et al.
Publicado: (2017)

Identification of G protein-coupled receptors in Schistosoma haematobium and S. mansoni by comparative genomics
por: Campos, Tulio D L, et al.
Publicado: (2014)

Bulinus truncatus transcriptome – a resource to enable molecular studies of snail and schistosome biology
por: Stroehlein, Andreas J., et al.
Publicado: (2021)

Phylogenetic Analysis of Mitogenomic Data Sets Resolves the Relationship of Seven Macropostrongyloides Species from Australian Macropodid and Vombatid Marsupials
por: Sukee, Tanapan, et al.
Publicado: (2020)

Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data
por: Sukee, Tanapan, et al.
Publicado: (2021)

Phylogenetic Relationships of the Strongyloid Nematodes of Australasian Marsupials Based on Mitochondrial Protein Sequences
por: Sukee, Tanapan, et al.
Publicado: (2022)

Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples
por: Santana, Bruna Nicoleti, et al.
Publicado: (2022)

Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
por: Marangi, Marianna, et al.
Publicado: (2015)

Improvement of Rotavirus Genotyping Method by Using the Semi-Nested Multiplex-PCR With New Primer Set
por: Fujii, Yoshiki, et al.
Publicado: (2019)

Comparative Transcriptomic Exploration Reveals Unique Molecular Adaptations of Neuropathogenic Trichobilharzia to Invade and Parasitize Its Avian Definitive Host
por: Leontovyč, Roman, et al.
Publicado: (2016)

CAP protein superfamily members in Toxocara canis
por: Stroehlein, Andreas J., et al.
Publicado: (2016)

Molecular evidence for distinct modes of nutrient acquisition between visceral and neurotropic schistosomes of birds
por: Leontovyč, Roman, et al.
Publicado: (2019)

A Sensitive and Specific PCR Based Method for Identification of Cryptosporidium Sp. Using New Primers from 18S Ribosomal RNA
por: Bairami Kuzehkanan, A, et al.
Publicado: (2011)

An Evaluation of Machine Learning Approaches for the Prediction of Essential Genes in Eukaryotes Using Protein Sequence-Derived Features()
por: Campos, Tulio L., et al.
Publicado: (2019)

Detection and Molecular Characterization of Cryptosporidium species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran using nested-PCR-RFLP
por: Manouchehri Naeini, K, et al.
Publicado: (2011)

Screening nested-PCR primer for ‘Candidatus Liberibacter asiaticus’ associated with citrus Huanglongbing and application in Hunan, China
por: Hong, Yanyun, et al.
Publicado: (2019)

Updating a New Semi-nested PCR Primer Pair for the Specific Detection of GII Norovirus in Oysters
por: Dong, Lei, et al.
Publicado: (2022)

Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11
por: Lin, Tingru, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_io8adqln8rog8nchapiplf6m58