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Sequence Evidence for the Presence of Two Tetrapyrrole Pathways in Euglena gracilis

Genes encoding enzymes of the tetrapyrrole biosynthetic pathway were searched within Euglena gracilis EST databases and 454 genome reads and their 5' end regions were sequenced when not available. Phylogenetic analyses and protein localization predictions support the hypothesis concerning the p...

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Autores principales: Kořený, Luděk, Oborník, Miroslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5654406/
https://www.ncbi.nlm.nih.gov/pubmed/21444293
http://dx.doi.org/10.1093/gbe/evr029
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author Kořený, Luděk
Oborník, Miroslav
author_facet Kořený, Luděk
Oborník, Miroslav
author_sort Kořený, Luděk
collection PubMed
description Genes encoding enzymes of the tetrapyrrole biosynthetic pathway were searched within Euglena gracilis EST databases and 454 genome reads and their 5' end regions were sequenced when not available. Phylogenetic analyses and protein localization predictions support the hypothesis concerning the presence of two separated tetrapyrrole pathways in E. gracilis. One of these pathways resembles the heme synthesis in primarily heterotrophic eukaryotes and was presumably present in the host cell prior to secondary endosymbiosis with a green alga. The second pathway is similar to the plastid-localized tetrapyrrole syntheses in plants and photosynthetic algae. It appears to be localized to the secondary plastid, presumably derived from an algal endosymbiont and probably serves only for the production of plastidial heme and chlorophyll. Thus, E. gracilis represents an evolutionary intermediate in a metabolic transformation of a primary heterotroph to a photoautotroph through secondary endosymbiosis. We propose here that the tetrapyrrole pathway serves as a highly informative marker for the evolution of plastids and plays a crucial role in the loss of plastids.
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spelling pubmed-56544062017-10-30 Sequence Evidence for the Presence of Two Tetrapyrrole Pathways in Euglena gracilis Kořený, Luděk Oborník, Miroslav Genome Biol Evol Letters Genes encoding enzymes of the tetrapyrrole biosynthetic pathway were searched within Euglena gracilis EST databases and 454 genome reads and their 5' end regions were sequenced when not available. Phylogenetic analyses and protein localization predictions support the hypothesis concerning the presence of two separated tetrapyrrole pathways in E. gracilis. One of these pathways resembles the heme synthesis in primarily heterotrophic eukaryotes and was presumably present in the host cell prior to secondary endosymbiosis with a green alga. The second pathway is similar to the plastid-localized tetrapyrrole syntheses in plants and photosynthetic algae. It appears to be localized to the secondary plastid, presumably derived from an algal endosymbiont and probably serves only for the production of plastidial heme and chlorophyll. Thus, E. gracilis represents an evolutionary intermediate in a metabolic transformation of a primary heterotroph to a photoautotroph through secondary endosymbiosis. We propose here that the tetrapyrrole pathway serves as a highly informative marker for the evolution of plastids and plays a crucial role in the loss of plastids. Oxford University Press 2011-03-27 /pmc/articles/PMC5654406/ /pubmed/21444293 http://dx.doi.org/10.1093/gbe/evr029 Text en © The Author(s) 2011. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Letters
Kořený, Luděk
Oborník, Miroslav
Sequence Evidence for the Presence of Two Tetrapyrrole Pathways in Euglena gracilis
title Sequence Evidence for the Presence of Two Tetrapyrrole Pathways in Euglena gracilis
title_full Sequence Evidence for the Presence of Two Tetrapyrrole Pathways in Euglena gracilis
title_fullStr Sequence Evidence for the Presence of Two Tetrapyrrole Pathways in Euglena gracilis
title_full_unstemmed Sequence Evidence for the Presence of Two Tetrapyrrole Pathways in Euglena gracilis
title_short Sequence Evidence for the Presence of Two Tetrapyrrole Pathways in Euglena gracilis
title_sort sequence evidence for the presence of two tetrapyrrole pathways in euglena gracilis
topic Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5654406/
https://www.ncbi.nlm.nih.gov/pubmed/21444293
http://dx.doi.org/10.1093/gbe/evr029
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