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Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants

Most soybean cultivars possess broad leaflets; however, a recessive allele on the Ln locus is known to cause the alteration of broad to narrow leaflets. The recessive allele ln has also been considered to increase the number of seeds per pod (NSP) and has the potential to improve yield. Recently, Gm...

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Autores principales: Sayama, Takashi, Tanabata, Takanari, Saruta, Masayasu, Yamada, Testsuya, Anai, Toyoaki, Kaga, Akito, Ishimoto, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5654458/
https://www.ncbi.nlm.nih.gov/pubmed/29085246
http://dx.doi.org/10.1270/jsbbs.16201
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author Sayama, Takashi
Tanabata, Takanari
Saruta, Masayasu
Yamada, Testsuya
Anai, Toyoaki
Kaga, Akito
Ishimoto, Masao
author_facet Sayama, Takashi
Tanabata, Takanari
Saruta, Masayasu
Yamada, Testsuya
Anai, Toyoaki
Kaga, Akito
Ishimoto, Masao
author_sort Sayama, Takashi
collection PubMed
description Most soybean cultivars possess broad leaflets; however, a recessive allele on the Ln locus is known to cause the alteration of broad to narrow leaflets. The recessive allele ln has also been considered to increase the number of seeds per pod (NSP) and has the potential to improve yield. Recently, Gm-JAG1 (Glyma20g25000), a gene controlling Ln, has been shown to complement leaf shape and silique length in Arabidopsis mutants. However, whether Gm-JAG1 is responsible for those traits in soybean is not yet known. In this study, we investigated the pleiotropic effect of soybean Ln gene on leaflet shape and NSP by using two independent soybean Gm-jag1 mutants and four ln near isogenic lines (NILs). The leaflet shape was evaluated using a leaf image analysis software, SmartLeaf, which was customized from SmartGrain. The leaflets of both the Gm-jag1 mutants were longer and narrower than those of the wild-type plants. Interestingly, the image analysis results clarified that the perimeter of the mutant leaflets did not change, although their leaflet area decreased. Furthermore, one mutant line with narrow leaflets showed significantly higher NSP than that in the wild (or Ln) genotype, indicating that soybean Ln gene pleiotropically controls leaflet shape and NSP.
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spelling pubmed-56544582017-10-30 Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants Sayama, Takashi Tanabata, Takanari Saruta, Masayasu Yamada, Testsuya Anai, Toyoaki Kaga, Akito Ishimoto, Masao Breed Sci Research Paper Most soybean cultivars possess broad leaflets; however, a recessive allele on the Ln locus is known to cause the alteration of broad to narrow leaflets. The recessive allele ln has also been considered to increase the number of seeds per pod (NSP) and has the potential to improve yield. Recently, Gm-JAG1 (Glyma20g25000), a gene controlling Ln, has been shown to complement leaf shape and silique length in Arabidopsis mutants. However, whether Gm-JAG1 is responsible for those traits in soybean is not yet known. In this study, we investigated the pleiotropic effect of soybean Ln gene on leaflet shape and NSP by using two independent soybean Gm-jag1 mutants and four ln near isogenic lines (NILs). The leaflet shape was evaluated using a leaf image analysis software, SmartLeaf, which was customized from SmartGrain. The leaflets of both the Gm-jag1 mutants were longer and narrower than those of the wild-type plants. Interestingly, the image analysis results clarified that the perimeter of the mutant leaflets did not change, although their leaflet area decreased. Furthermore, one mutant line with narrow leaflets showed significantly higher NSP than that in the wild (or Ln) genotype, indicating that soybean Ln gene pleiotropically controls leaflet shape and NSP. Japanese Society of Breeding 2017-09 2017-07-28 /pmc/articles/PMC5654458/ /pubmed/29085246 http://dx.doi.org/10.1270/jsbbs.16201 Text en Copyright © 2017 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Sayama, Takashi
Tanabata, Takanari
Saruta, Masayasu
Yamada, Testsuya
Anai, Toyoaki
Kaga, Akito
Ishimoto, Masao
Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants
title Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants
title_full Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants
title_fullStr Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants
title_full_unstemmed Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants
title_short Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants
title_sort confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the ln gene in induced soybean mutants
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5654458/
https://www.ncbi.nlm.nih.gov/pubmed/29085246
http://dx.doi.org/10.1270/jsbbs.16201
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