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Genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents

Little is known about evolutionary drivers of microbial populations in the warm subseafloor of deep-sea hydrothermal vents. Here we reconstruct 73 metagenome-assembled genomes (MAGs) from two geochemically distinct vent fields in the Mid-Cayman Rise to investigate patterns of genomic variation withi...

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Autores principales: Anderson, Rika E., Reveillaud, Julie, Reddington, Emily, Delmont, Tom O., Eren, A. Murat, McDermott, Jill M., Seewald, Jeff S., Huber, Julie A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655027/
https://www.ncbi.nlm.nih.gov/pubmed/29066755
http://dx.doi.org/10.1038/s41467-017-01228-6
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author Anderson, Rika E.
Reveillaud, Julie
Reddington, Emily
Delmont, Tom O.
Eren, A. Murat
McDermott, Jill M.
Seewald, Jeff S.
Huber, Julie A.
author_facet Anderson, Rika E.
Reveillaud, Julie
Reddington, Emily
Delmont, Tom O.
Eren, A. Murat
McDermott, Jill M.
Seewald, Jeff S.
Huber, Julie A.
author_sort Anderson, Rika E.
collection PubMed
description Little is known about evolutionary drivers of microbial populations in the warm subseafloor of deep-sea hydrothermal vents. Here we reconstruct 73 metagenome-assembled genomes (MAGs) from two geochemically distinct vent fields in the Mid-Cayman Rise to investigate patterns of genomic variation within subseafloor populations. Low-abundance populations with high intra-population diversity coexist alongside high-abundance populations with low genomic diversity, with taxonomic differences in patterns of genomic variation between the mafic Piccard and ultramafic Von Damm vent fields. Populations from Piccard are significantly enriched in nonsynonymous mutations, suggesting stronger purifying selection in Von Damm relative to Piccard. Comparison of nine Sulfurovum MAGs reveals two high-coverage, low-diversity MAGs from Piccard enriched in unique genes related to the cellular membrane, suggesting these populations were subject to distinct evolutionary pressures that may correlate with genes related to nutrient uptake, biofilm formation, or viral invasion. These results are consistent with distinct evolutionary histories between geochemically different vent fields, with implications for understanding evolutionary processes in subseafloor microbial populations.
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spelling pubmed-56550272017-10-26 Genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents Anderson, Rika E. Reveillaud, Julie Reddington, Emily Delmont, Tom O. Eren, A. Murat McDermott, Jill M. Seewald, Jeff S. Huber, Julie A. Nat Commun Article Little is known about evolutionary drivers of microbial populations in the warm subseafloor of deep-sea hydrothermal vents. Here we reconstruct 73 metagenome-assembled genomes (MAGs) from two geochemically distinct vent fields in the Mid-Cayman Rise to investigate patterns of genomic variation within subseafloor populations. Low-abundance populations with high intra-population diversity coexist alongside high-abundance populations with low genomic diversity, with taxonomic differences in patterns of genomic variation between the mafic Piccard and ultramafic Von Damm vent fields. Populations from Piccard are significantly enriched in nonsynonymous mutations, suggesting stronger purifying selection in Von Damm relative to Piccard. Comparison of nine Sulfurovum MAGs reveals two high-coverage, low-diversity MAGs from Piccard enriched in unique genes related to the cellular membrane, suggesting these populations were subject to distinct evolutionary pressures that may correlate with genes related to nutrient uptake, biofilm formation, or viral invasion. These results are consistent with distinct evolutionary histories between geochemically different vent fields, with implications for understanding evolutionary processes in subseafloor microbial populations. Nature Publishing Group UK 2017-10-24 /pmc/articles/PMC5655027/ /pubmed/29066755 http://dx.doi.org/10.1038/s41467-017-01228-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Anderson, Rika E.
Reveillaud, Julie
Reddington, Emily
Delmont, Tom O.
Eren, A. Murat
McDermott, Jill M.
Seewald, Jeff S.
Huber, Julie A.
Genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents
title Genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents
title_full Genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents
title_fullStr Genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents
title_full_unstemmed Genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents
title_short Genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents
title_sort genomic variation in microbial populations inhabiting the marine subseafloor at deep-sea hydrothermal vents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655027/
https://www.ncbi.nlm.nih.gov/pubmed/29066755
http://dx.doi.org/10.1038/s41467-017-01228-6
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