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Correlation between resistance mechanisms in Staphylococcus aureus and cell wall and septum thickening

PURPOSE: The aim of the present study is to examine cell wall and septum thickening of methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant S. aureus (MRSA), and methicillin- and linezolid-resistant S. aureus (MLRSA) isolates by transmission electron microscopy to correlate the...

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Autores principales: García, Ana Belén, Viñuela-Prieto, José Manuel, López-González, Laura, Candel, Francisco Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655128/
https://www.ncbi.nlm.nih.gov/pubmed/29089777
http://dx.doi.org/10.2147/IDR.S146748
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author García, Ana Belén
Viñuela-Prieto, José Manuel
López-González, Laura
Candel, Francisco Javier
author_facet García, Ana Belén
Viñuela-Prieto, José Manuel
López-González, Laura
Candel, Francisco Javier
author_sort García, Ana Belén
collection PubMed
description PURPOSE: The aim of the present study is to examine cell wall and septum thickening of methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant S. aureus (MRSA), and methicillin- and linezolid-resistant S. aureus (MLRSA) isolates by transmission electron microscopy to correlate the association of resistance mechanisms with major changes in the morphology of membrane or septum. MATERIALS AND METHODS: MSSA, MRSA, and MLRSA strains obtained from clinical samples of an outbreak that occurred in 2010 at the Intensive Care Unit of our Hospital were thawed and sown at 37°C in blood agar overnight. After that, they were washed, pelleted, and treated with a fixer solution. Pellets were dehydrated and finally embedded in resin. Transmission electron microscopy was used to characterize cell wall and septum thickening in all isolates. The comparison between the measurements obtained for each group was performed by a Kruskal–Wallis test and a post hoc Dunn–Bonferroni’s pairwise comparison method. RESULTS: Differences in cell wall and septum thickness were statistically significant (P<0.001 and P<0.001, respectively) between the three groups. Moreover, significant differences were detected in wall and septum thickness between the MSSA and MRSA strains (P<0.001 and P<0.001, respectively) and between the MSSA and MLRSA strains (P<0.001 and P<0.001, respectively) but not between the MRSA and MLRSA strains (P=0.386 and P=0.117). CONCLUSION: In this analysis, we correlate the resistance mediated by alterations in the cell membrane of S. aureus (methicillin-resistant, for example) with a greater thickness of the wall or septum. The resistance added to linezolid did not determine significant changes in the characteristics of the wall or septum with respect to those strains resistant only to methicillin.
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spelling pubmed-56551282017-10-31 Correlation between resistance mechanisms in Staphylococcus aureus and cell wall and septum thickening García, Ana Belén Viñuela-Prieto, José Manuel López-González, Laura Candel, Francisco Javier Infect Drug Resist Original Research PURPOSE: The aim of the present study is to examine cell wall and septum thickening of methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant S. aureus (MRSA), and methicillin- and linezolid-resistant S. aureus (MLRSA) isolates by transmission electron microscopy to correlate the association of resistance mechanisms with major changes in the morphology of membrane or septum. MATERIALS AND METHODS: MSSA, MRSA, and MLRSA strains obtained from clinical samples of an outbreak that occurred in 2010 at the Intensive Care Unit of our Hospital were thawed and sown at 37°C in blood agar overnight. After that, they were washed, pelleted, and treated with a fixer solution. Pellets were dehydrated and finally embedded in resin. Transmission electron microscopy was used to characterize cell wall and septum thickening in all isolates. The comparison between the measurements obtained for each group was performed by a Kruskal–Wallis test and a post hoc Dunn–Bonferroni’s pairwise comparison method. RESULTS: Differences in cell wall and septum thickness were statistically significant (P<0.001 and P<0.001, respectively) between the three groups. Moreover, significant differences were detected in wall and septum thickness between the MSSA and MRSA strains (P<0.001 and P<0.001, respectively) and between the MSSA and MLRSA strains (P<0.001 and P<0.001, respectively) but not between the MRSA and MLRSA strains (P=0.386 and P=0.117). CONCLUSION: In this analysis, we correlate the resistance mediated by alterations in the cell membrane of S. aureus (methicillin-resistant, for example) with a greater thickness of the wall or septum. The resistance added to linezolid did not determine significant changes in the characteristics of the wall or septum with respect to those strains resistant only to methicillin. Dove Medical Press 2017-10-17 /pmc/articles/PMC5655128/ /pubmed/29089777 http://dx.doi.org/10.2147/IDR.S146748 Text en © 2017 García et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
García, Ana Belén
Viñuela-Prieto, José Manuel
López-González, Laura
Candel, Francisco Javier
Correlation between resistance mechanisms in Staphylococcus aureus and cell wall and septum thickening
title Correlation between resistance mechanisms in Staphylococcus aureus and cell wall and septum thickening
title_full Correlation between resistance mechanisms in Staphylococcus aureus and cell wall and septum thickening
title_fullStr Correlation between resistance mechanisms in Staphylococcus aureus and cell wall and septum thickening
title_full_unstemmed Correlation between resistance mechanisms in Staphylococcus aureus and cell wall and septum thickening
title_short Correlation between resistance mechanisms in Staphylococcus aureus and cell wall and septum thickening
title_sort correlation between resistance mechanisms in staphylococcus aureus and cell wall and septum thickening
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655128/
https://www.ncbi.nlm.nih.gov/pubmed/29089777
http://dx.doi.org/10.2147/IDR.S146748
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