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Transcription factor TFEB cell-autonomously modulates susceptibility to intestinal epithelial cell injury in vivo

Understanding the transcription factors that modulate epithelial resistance to injury is necessary for understanding intestinal homeostasis and injury repair processes. Recently, transcription factor EB (TFEB) was implicated in expression of autophagy and host defense genes in nematodes and mammalia...

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Detalles Bibliográficos
Autores principales: Murano, Tatsuro, Najibi, Mehran, Paulus, Geraldine L. C., Adiliaghdam, Fatemeh, Valencia-Guerrero, Aida, Selig, Martin, Wang, Xiaofei, Jeffrey, Kate, Xavier, Ramnik J., Lassen, Kara G., Irazoqui, Javier E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655326/
https://www.ncbi.nlm.nih.gov/pubmed/29066772
http://dx.doi.org/10.1038/s41598-017-14370-4
Descripción
Sumario:Understanding the transcription factors that modulate epithelial resistance to injury is necessary for understanding intestinal homeostasis and injury repair processes. Recently, transcription factor EB (TFEB) was implicated in expression of autophagy and host defense genes in nematodes and mammalian cells. However, the in vivo roles of TFEB in the mammalian intestinal epithelium were not known. Here, we used mice with a conditional deletion of Tfeb in the intestinal epithelium (Tfeb (ΔIEC)) to examine its importance in defense against injury. Unperturbed Tfeb (ΔIEC) mice exhibited grossly normal intestinal epithelia, except for a defect in Paneth cell granules. Tfeb (ΔIEC) mice exhibited lower levels of lipoprotein ApoA1 expression, which is downregulated in Crohn’s disease patients and causally linked to colitis susceptibility. Upon environmental epithelial injury using dextran sodium sulfate (DSS), Tfeb (ΔIEC) mice exhibited exaggerated colitis. Thus, our study reveals that TFEB is critical for resistance to intestinal epithelial cell injury, potentially mediated by APOA1.