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Drosophila VAMP7 regulates Wingless intracellular trafficking
Drosophila Wingless (Wg) is a morphogen that determines cell fate during development. Previous studies have shown that endocytic pathways regulate Wg trafficking and signaling. Here, we showed that loss of vamp7, a gene required for vesicle fusion, dramatically increased Wg levels and decreased Wg s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655445/ https://www.ncbi.nlm.nih.gov/pubmed/29065163 http://dx.doi.org/10.1371/journal.pone.0186938 |
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author | Gao, Han He, Fang Lin, Xinhua Wu, Yihui |
author_facet | Gao, Han He, Fang Lin, Xinhua Wu, Yihui |
author_sort | Gao, Han |
collection | PubMed |
description | Drosophila Wingless (Wg) is a morphogen that determines cell fate during development. Previous studies have shown that endocytic pathways regulate Wg trafficking and signaling. Here, we showed that loss of vamp7, a gene required for vesicle fusion, dramatically increased Wg levels and decreased Wg signaling. Interestingly, we found that levels of Dally-like (Dlp), a glypican that can interact with Wg to suppress Wg signaling at the dorsoventral boundary of the Drosophila wing, were also increased in vamp7 mutant cells. Moreover, Wg puncta in Rab4-dependent recycling endosomes were Dlp positive. We hypothesize that VAMP7 is required for Wg intracellular trafficking and the accumulation of Wg in Rab4-dependent recycling endosomes might affect Wg signaling. |
format | Online Article Text |
id | pubmed-5655445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56554452017-11-09 Drosophila VAMP7 regulates Wingless intracellular trafficking Gao, Han He, Fang Lin, Xinhua Wu, Yihui PLoS One Research Article Drosophila Wingless (Wg) is a morphogen that determines cell fate during development. Previous studies have shown that endocytic pathways regulate Wg trafficking and signaling. Here, we showed that loss of vamp7, a gene required for vesicle fusion, dramatically increased Wg levels and decreased Wg signaling. Interestingly, we found that levels of Dally-like (Dlp), a glypican that can interact with Wg to suppress Wg signaling at the dorsoventral boundary of the Drosophila wing, were also increased in vamp7 mutant cells. Moreover, Wg puncta in Rab4-dependent recycling endosomes were Dlp positive. We hypothesize that VAMP7 is required for Wg intracellular trafficking and the accumulation of Wg in Rab4-dependent recycling endosomes might affect Wg signaling. Public Library of Science 2017-10-24 /pmc/articles/PMC5655445/ /pubmed/29065163 http://dx.doi.org/10.1371/journal.pone.0186938 Text en © 2017 Gao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gao, Han He, Fang Lin, Xinhua Wu, Yihui Drosophila VAMP7 regulates Wingless intracellular trafficking |
title | Drosophila VAMP7 regulates Wingless intracellular trafficking |
title_full | Drosophila VAMP7 regulates Wingless intracellular trafficking |
title_fullStr | Drosophila VAMP7 regulates Wingless intracellular trafficking |
title_full_unstemmed | Drosophila VAMP7 regulates Wingless intracellular trafficking |
title_short | Drosophila VAMP7 regulates Wingless intracellular trafficking |
title_sort | drosophila vamp7 regulates wingless intracellular trafficking |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655445/ https://www.ncbi.nlm.nih.gov/pubmed/29065163 http://dx.doi.org/10.1371/journal.pone.0186938 |
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