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Drosophila VAMP7 regulates Wingless intracellular trafficking

Drosophila Wingless (Wg) is a morphogen that determines cell fate during development. Previous studies have shown that endocytic pathways regulate Wg trafficking and signaling. Here, we showed that loss of vamp7, a gene required for vesicle fusion, dramatically increased Wg levels and decreased Wg s...

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Detalles Bibliográficos
Autores principales: Gao, Han, He, Fang, Lin, Xinhua, Wu, Yihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655445/
https://www.ncbi.nlm.nih.gov/pubmed/29065163
http://dx.doi.org/10.1371/journal.pone.0186938
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author Gao, Han
He, Fang
Lin, Xinhua
Wu, Yihui
author_facet Gao, Han
He, Fang
Lin, Xinhua
Wu, Yihui
author_sort Gao, Han
collection PubMed
description Drosophila Wingless (Wg) is a morphogen that determines cell fate during development. Previous studies have shown that endocytic pathways regulate Wg trafficking and signaling. Here, we showed that loss of vamp7, a gene required for vesicle fusion, dramatically increased Wg levels and decreased Wg signaling. Interestingly, we found that levels of Dally-like (Dlp), a glypican that can interact with Wg to suppress Wg signaling at the dorsoventral boundary of the Drosophila wing, were also increased in vamp7 mutant cells. Moreover, Wg puncta in Rab4-dependent recycling endosomes were Dlp positive. We hypothesize that VAMP7 is required for Wg intracellular trafficking and the accumulation of Wg in Rab4-dependent recycling endosomes might affect Wg signaling.
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spelling pubmed-56554452017-11-09 Drosophila VAMP7 regulates Wingless intracellular trafficking Gao, Han He, Fang Lin, Xinhua Wu, Yihui PLoS One Research Article Drosophila Wingless (Wg) is a morphogen that determines cell fate during development. Previous studies have shown that endocytic pathways regulate Wg trafficking and signaling. Here, we showed that loss of vamp7, a gene required for vesicle fusion, dramatically increased Wg levels and decreased Wg signaling. Interestingly, we found that levels of Dally-like (Dlp), a glypican that can interact with Wg to suppress Wg signaling at the dorsoventral boundary of the Drosophila wing, were also increased in vamp7 mutant cells. Moreover, Wg puncta in Rab4-dependent recycling endosomes were Dlp positive. We hypothesize that VAMP7 is required for Wg intracellular trafficking and the accumulation of Wg in Rab4-dependent recycling endosomes might affect Wg signaling. Public Library of Science 2017-10-24 /pmc/articles/PMC5655445/ /pubmed/29065163 http://dx.doi.org/10.1371/journal.pone.0186938 Text en © 2017 Gao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gao, Han
He, Fang
Lin, Xinhua
Wu, Yihui
Drosophila VAMP7 regulates Wingless intracellular trafficking
title Drosophila VAMP7 regulates Wingless intracellular trafficking
title_full Drosophila VAMP7 regulates Wingless intracellular trafficking
title_fullStr Drosophila VAMP7 regulates Wingless intracellular trafficking
title_full_unstemmed Drosophila VAMP7 regulates Wingless intracellular trafficking
title_short Drosophila VAMP7 regulates Wingless intracellular trafficking
title_sort drosophila vamp7 regulates wingless intracellular trafficking
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655445/
https://www.ncbi.nlm.nih.gov/pubmed/29065163
http://dx.doi.org/10.1371/journal.pone.0186938
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