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RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma

RAD52 motif containing 1 (RDM1) encodes the RAD52 protein involved in DNA double-strand break repair and recombination events. However, the importance of RDM1 in papillary thyroid carcinoma (PTC) is largely unknown. In the present study, we examined the role of RDM1 in thyroid cancer. The RDM1 expre...

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Autores principales: Li, Wei, Huang, Qing, Sun, Danyang, Zhang, Guizhi, Tan, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655686/
https://www.ncbi.nlm.nih.gov/pubmed/28939762
http://dx.doi.org/10.1530/EC-17-0209
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author Li, Wei
Huang, Qing
Sun, Danyang
Zhang, Guizhi
Tan, Jian
author_facet Li, Wei
Huang, Qing
Sun, Danyang
Zhang, Guizhi
Tan, Jian
author_sort Li, Wei
collection PubMed
description RAD52 motif containing 1 (RDM1) encodes the RAD52 protein involved in DNA double-strand break repair and recombination events. However, the importance of RDM1 in papillary thyroid carcinoma (PTC) is largely unknown. In the present study, we examined the role of RDM1 in thyroid cancer. The RDM1 expression in PTC patients was examined using immunohistochemistry. The expression levels of RDM1 mRNA in thyroid cancer cells were measured by quantitative real-time PCR (qRT-PCR). Lentivirus-mediated small interfering RNAs (siRNAs) were used to knock down the RDM1 expression in the K1 and TPC1 cells. Then, changes in the RDM1 target gene expression were determined by qRT-PCR and Western blot. Cell proliferation was examined by a high content screening assay. Cell cycle distribution and apoptosis were detected by flow cytometric analysis and MTT analysis. We showed that the RDM1 expression was higher in PTC tissue compared to pericarcinous tissue. RDM1 mRNA was found to be expressed by qRT-PCR. Using a lentivirus-based RNA interference (RNAi) approach, the RDM1 expression was significantly inhibited. The inhibition of RDM1 expression by RNAi significantly impaired cell proliferation, increased apoptosis and arrested cells in the G2/M phase. These data showed that RDM1 was highly expressed in PTC tissue and thyroid cancer cell lines. Moreover, RDM1 may play an important role in cell proliferation, cell cycle distribution and apoptosis of human PTC cells.
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spelling pubmed-56556862017-10-30 RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma Li, Wei Huang, Qing Sun, Danyang Zhang, Guizhi Tan, Jian Endocr Connect Research RAD52 motif containing 1 (RDM1) encodes the RAD52 protein involved in DNA double-strand break repair and recombination events. However, the importance of RDM1 in papillary thyroid carcinoma (PTC) is largely unknown. In the present study, we examined the role of RDM1 in thyroid cancer. The RDM1 expression in PTC patients was examined using immunohistochemistry. The expression levels of RDM1 mRNA in thyroid cancer cells were measured by quantitative real-time PCR (qRT-PCR). Lentivirus-mediated small interfering RNAs (siRNAs) were used to knock down the RDM1 expression in the K1 and TPC1 cells. Then, changes in the RDM1 target gene expression were determined by qRT-PCR and Western blot. Cell proliferation was examined by a high content screening assay. Cell cycle distribution and apoptosis were detected by flow cytometric analysis and MTT analysis. We showed that the RDM1 expression was higher in PTC tissue compared to pericarcinous tissue. RDM1 mRNA was found to be expressed by qRT-PCR. Using a lentivirus-based RNA interference (RNAi) approach, the RDM1 expression was significantly inhibited. The inhibition of RDM1 expression by RNAi significantly impaired cell proliferation, increased apoptosis and arrested cells in the G2/M phase. These data showed that RDM1 was highly expressed in PTC tissue and thyroid cancer cell lines. Moreover, RDM1 may play an important role in cell proliferation, cell cycle distribution and apoptosis of human PTC cells. Bioscientifica Ltd 2017-09-22 /pmc/articles/PMC5655686/ /pubmed/28939762 http://dx.doi.org/10.1530/EC-17-0209 Text en © 2017 The authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Research
Li, Wei
Huang, Qing
Sun, Danyang
Zhang, Guizhi
Tan, Jian
RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma
title RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma
title_full RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma
title_fullStr RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma
title_full_unstemmed RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma
title_short RDM1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma
title_sort rdm1 gene overexpression represents a therapeutic target in papillary thyroid carcinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655686/
https://www.ncbi.nlm.nih.gov/pubmed/28939762
http://dx.doi.org/10.1530/EC-17-0209
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