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Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco

BACKGROUND: The C-repeat-binding factors/DRE-binding factors (CBF/DREBs) comprise a key transcription factor family involved in plant stress tolerance. Yet, there is limited information about switchgrass DREB genes and their functional roles. RESULTS: In this study, four cold-inducible PvDREB1s were...

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Autores principales: Wen, Wuwu, Xie, Zheni, Yu, Guohui, Zhao, Chengliang, Zhang, Jing, Huang, Linkai, Xu, Bin, Huang, Bingru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655823/
https://www.ncbi.nlm.nih.gov/pubmed/29123463
http://dx.doi.org/10.1186/s41065-017-0050-4
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author Wen, Wuwu
Xie, Zheni
Yu, Guohui
Zhao, Chengliang
Zhang, Jing
Huang, Linkai
Xu, Bin
Huang, Bingru
author_facet Wen, Wuwu
Xie, Zheni
Yu, Guohui
Zhao, Chengliang
Zhang, Jing
Huang, Linkai
Xu, Bin
Huang, Bingru
author_sort Wen, Wuwu
collection PubMed
description BACKGROUND: The C-repeat-binding factors/DRE-binding factors (CBF/DREBs) comprise a key transcription factor family involved in plant stress tolerance. Yet, there is limited information about switchgrass DREB genes and their functional roles. RESULTS: In this study, four cold-inducible PvDREB1s were identified from switchgrass (Panicum virgatum), among which PvDREB1C was the one responded to cold stress later than the other three PvDREB1s. Yet, ectopic overexpression of PvDREB1C led to significantly compromised, instead of improved cold tolerance in transgenic tobacco. On the other hand, PvDREB1C was transcriptionally down-regulated in response to salt stress, but overexpression of PvDREB1C improved plant salt tolerance in transgenic tobacco. The improved salt tolerance was associated with increased K(+)/Na(+) ratio and Ca(2+) content, higher cellular osmotic potential, and activation of stress-related functional genes in the leaves of transgenic plants under salt stress. CONCLUSIONS: The current results implied that PvDREB1C played opposite roles in plant cold and salt tolerance. Although DREB1s were known as positive stress regulators, particular attentions shall be paid to their potential negative regulatory role(s). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s41065-017-0050-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-56558232017-11-09 Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco Wen, Wuwu Xie, Zheni Yu, Guohui Zhao, Chengliang Zhang, Jing Huang, Linkai Xu, Bin Huang, Bingru Hereditas Research BACKGROUND: The C-repeat-binding factors/DRE-binding factors (CBF/DREBs) comprise a key transcription factor family involved in plant stress tolerance. Yet, there is limited information about switchgrass DREB genes and their functional roles. RESULTS: In this study, four cold-inducible PvDREB1s were identified from switchgrass (Panicum virgatum), among which PvDREB1C was the one responded to cold stress later than the other three PvDREB1s. Yet, ectopic overexpression of PvDREB1C led to significantly compromised, instead of improved cold tolerance in transgenic tobacco. On the other hand, PvDREB1C was transcriptionally down-regulated in response to salt stress, but overexpression of PvDREB1C improved plant salt tolerance in transgenic tobacco. The improved salt tolerance was associated with increased K(+)/Na(+) ratio and Ca(2+) content, higher cellular osmotic potential, and activation of stress-related functional genes in the leaves of transgenic plants under salt stress. CONCLUSIONS: The current results implied that PvDREB1C played opposite roles in plant cold and salt tolerance. Although DREB1s were known as positive stress regulators, particular attentions shall be paid to their potential negative regulatory role(s). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s41065-017-0050-4) contains supplementary material, which is available to authorized users. BioMed Central 2017-10-24 /pmc/articles/PMC5655823/ /pubmed/29123463 http://dx.doi.org/10.1186/s41065-017-0050-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wen, Wuwu
Xie, Zheni
Yu, Guohui
Zhao, Chengliang
Zhang, Jing
Huang, Linkai
Xu, Bin
Huang, Bingru
Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco
title Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco
title_full Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco
title_fullStr Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco
title_full_unstemmed Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco
title_short Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco
title_sort switchgrass pvdreb1c plays opposite roles in plant cold and salt tolerance in transgenic tobacco
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655823/
https://www.ncbi.nlm.nih.gov/pubmed/29123463
http://dx.doi.org/10.1186/s41065-017-0050-4
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