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Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco
BACKGROUND: The C-repeat-binding factors/DRE-binding factors (CBF/DREBs) comprise a key transcription factor family involved in plant stress tolerance. Yet, there is limited information about switchgrass DREB genes and their functional roles. RESULTS: In this study, four cold-inducible PvDREB1s were...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655823/ https://www.ncbi.nlm.nih.gov/pubmed/29123463 http://dx.doi.org/10.1186/s41065-017-0050-4 |
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author | Wen, Wuwu Xie, Zheni Yu, Guohui Zhao, Chengliang Zhang, Jing Huang, Linkai Xu, Bin Huang, Bingru |
author_facet | Wen, Wuwu Xie, Zheni Yu, Guohui Zhao, Chengliang Zhang, Jing Huang, Linkai Xu, Bin Huang, Bingru |
author_sort | Wen, Wuwu |
collection | PubMed |
description | BACKGROUND: The C-repeat-binding factors/DRE-binding factors (CBF/DREBs) comprise a key transcription factor family involved in plant stress tolerance. Yet, there is limited information about switchgrass DREB genes and their functional roles. RESULTS: In this study, four cold-inducible PvDREB1s were identified from switchgrass (Panicum virgatum), among which PvDREB1C was the one responded to cold stress later than the other three PvDREB1s. Yet, ectopic overexpression of PvDREB1C led to significantly compromised, instead of improved cold tolerance in transgenic tobacco. On the other hand, PvDREB1C was transcriptionally down-regulated in response to salt stress, but overexpression of PvDREB1C improved plant salt tolerance in transgenic tobacco. The improved salt tolerance was associated with increased K(+)/Na(+) ratio and Ca(2+) content, higher cellular osmotic potential, and activation of stress-related functional genes in the leaves of transgenic plants under salt stress. CONCLUSIONS: The current results implied that PvDREB1C played opposite roles in plant cold and salt tolerance. Although DREB1s were known as positive stress regulators, particular attentions shall be paid to their potential negative regulatory role(s). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s41065-017-0050-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5655823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-56558232017-11-09 Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco Wen, Wuwu Xie, Zheni Yu, Guohui Zhao, Chengliang Zhang, Jing Huang, Linkai Xu, Bin Huang, Bingru Hereditas Research BACKGROUND: The C-repeat-binding factors/DRE-binding factors (CBF/DREBs) comprise a key transcription factor family involved in plant stress tolerance. Yet, there is limited information about switchgrass DREB genes and their functional roles. RESULTS: In this study, four cold-inducible PvDREB1s were identified from switchgrass (Panicum virgatum), among which PvDREB1C was the one responded to cold stress later than the other three PvDREB1s. Yet, ectopic overexpression of PvDREB1C led to significantly compromised, instead of improved cold tolerance in transgenic tobacco. On the other hand, PvDREB1C was transcriptionally down-regulated in response to salt stress, but overexpression of PvDREB1C improved plant salt tolerance in transgenic tobacco. The improved salt tolerance was associated with increased K(+)/Na(+) ratio and Ca(2+) content, higher cellular osmotic potential, and activation of stress-related functional genes in the leaves of transgenic plants under salt stress. CONCLUSIONS: The current results implied that PvDREB1C played opposite roles in plant cold and salt tolerance. Although DREB1s were known as positive stress regulators, particular attentions shall be paid to their potential negative regulatory role(s). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s41065-017-0050-4) contains supplementary material, which is available to authorized users. BioMed Central 2017-10-24 /pmc/articles/PMC5655823/ /pubmed/29123463 http://dx.doi.org/10.1186/s41065-017-0050-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Wen, Wuwu Xie, Zheni Yu, Guohui Zhao, Chengliang Zhang, Jing Huang, Linkai Xu, Bin Huang, Bingru Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco |
title | Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco |
title_full | Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco |
title_fullStr | Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco |
title_full_unstemmed | Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco |
title_short | Switchgrass PvDREB1C plays opposite roles in plant cold and salt tolerance in transgenic tobacco |
title_sort | switchgrass pvdreb1c plays opposite roles in plant cold and salt tolerance in transgenic tobacco |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5655823/ https://www.ncbi.nlm.nih.gov/pubmed/29123463 http://dx.doi.org/10.1186/s41065-017-0050-4 |
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