Chloromethyl-triazole: a new motif for site-selective pseudo-acylation of proteins

Rapid, site-selective modification of cysteine residues with chloromethyl-triazole derivatives generates pseudo-acyl sLys motifs, mimicking important post-translational modifications. Near-native biotinylation of peptide and protein substrates is shown to be site-selective and modified histone H4 re...

Descripción completa

Detalles Bibliográficos
Autores principales: Brewster, Richard C., Gavins, Georgina C., Günthardt, Barbara, Farr, Sarah, Webb, Kimberly M., Voigt, Philipp, Hulme, Alison N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656099/
https://www.ncbi.nlm.nih.gov/pubmed/27722332
http://dx.doi.org/10.1039/c6cc06801d
Descripción
Sumario:Rapid, site-selective modification of cysteine residues with chloromethyl-triazole derivatives generates pseudo-acyl sLys motifs, mimicking important post-translational modifications. Near-native biotinylation of peptide and protein substrates is shown to be site-selective and modified histone H4 retains functional activity.

Ejemplares similares