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Anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of Oldenlandia affinis (R. & S.) D.C. (Rubiaceae)

BACKGROUND: Oldenlandia affinis, commonly called ‘kalata-kalata’, a versatile plant used locally to treat malaria fever in some parts of sub-Saharan Africa was investigated for anti-plasmodial and anti-inflammatory activities. OBJECTIVE: The study was designed to evaluate the antiplasmodial as well...

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Autores principales: Nworu, Chukwuemeka Sylvester, Ejikeme, Tochukwu Ifenyinwa, Ezike, Adaobi Chioma, Ndu, Okechukwu, Akunne, Theophine Chinwuba, Onyeto, Collins Azubuike, Okpalanduka, Paul, Akah, Peter Achunike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Makerere Medical School 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656185/
https://www.ncbi.nlm.nih.gov/pubmed/29085411
http://dx.doi.org/10.4314/ahs.v17i3.26
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author Nworu, Chukwuemeka Sylvester
Ejikeme, Tochukwu Ifenyinwa
Ezike, Adaobi Chioma
Ndu, Okechukwu
Akunne, Theophine Chinwuba
Onyeto, Collins Azubuike
Okpalanduka, Paul
Akah, Peter Achunike
author_facet Nworu, Chukwuemeka Sylvester
Ejikeme, Tochukwu Ifenyinwa
Ezike, Adaobi Chioma
Ndu, Okechukwu
Akunne, Theophine Chinwuba
Onyeto, Collins Azubuike
Okpalanduka, Paul
Akah, Peter Achunike
author_sort Nworu, Chukwuemeka Sylvester
collection PubMed
description BACKGROUND: Oldenlandia affinis, commonly called ‘kalata-kalata’, a versatile plant used locally to treat malaria fever in some parts of sub-Saharan Africa was investigated for anti-plasmodial and anti-inflammatory activities. OBJECTIVE: The study was designed to evaluate the antiplasmodial as well as anti-inflammatory activities of whole extract and cyclotide-rich fraction of Oldenlandia affinis METHOD: The dichloromethane-methanol extract (ODE) of the plant, O. affinis was investigated for suppressive and curative antiplasmodial activities against Plasmodium berghei in mice. ODE and the cyclotide-rich fraction (CRF) was investigated for chronic and acute anti-inflammatory activities in rat models of inflammation. Inhibition of pro-inflammatory mediators was studied in RAW264.7 macrophages. RESULTS: ODE exhibited significant (p<0.05) reduction in mean parasitaemia in both the suppressive and curative models of Plasmodium berghei infection in mice.Administration of ODE(100, 200, or 400 mg/kg) and CRF (100, 200, or 400 mg/kg) produced significant inhibition of rodent models of acute and chronic inflammation . This observation is supported by the significant (P<0.05) inhibition of pro-inflammatory mediators, inducible nitric oxide (iNO) and tumour necrosis factor-alpha (TNF-α), and the reactive radical scavenging activities in RAW264.7 macrophages. CONCLUSION: These findings could explain, at least in part, the successes reported in the use of the herb, Oldenlandia affinis in the traditional treatment of malaria fever
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spelling pubmed-56561852017-10-30 Anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of Oldenlandia affinis (R. & S.) D.C. (Rubiaceae) Nworu, Chukwuemeka Sylvester Ejikeme, Tochukwu Ifenyinwa Ezike, Adaobi Chioma Ndu, Okechukwu Akunne, Theophine Chinwuba Onyeto, Collins Azubuike Okpalanduka, Paul Akah, Peter Achunike Afr Health Sci Articles BACKGROUND: Oldenlandia affinis, commonly called ‘kalata-kalata’, a versatile plant used locally to treat malaria fever in some parts of sub-Saharan Africa was investigated for anti-plasmodial and anti-inflammatory activities. OBJECTIVE: The study was designed to evaluate the antiplasmodial as well as anti-inflammatory activities of whole extract and cyclotide-rich fraction of Oldenlandia affinis METHOD: The dichloromethane-methanol extract (ODE) of the plant, O. affinis was investigated for suppressive and curative antiplasmodial activities against Plasmodium berghei in mice. ODE and the cyclotide-rich fraction (CRF) was investigated for chronic and acute anti-inflammatory activities in rat models of inflammation. Inhibition of pro-inflammatory mediators was studied in RAW264.7 macrophages. RESULTS: ODE exhibited significant (p<0.05) reduction in mean parasitaemia in both the suppressive and curative models of Plasmodium berghei infection in mice.Administration of ODE(100, 200, or 400 mg/kg) and CRF (100, 200, or 400 mg/kg) produced significant inhibition of rodent models of acute and chronic inflammation . This observation is supported by the significant (P<0.05) inhibition of pro-inflammatory mediators, inducible nitric oxide (iNO) and tumour necrosis factor-alpha (TNF-α), and the reactive radical scavenging activities in RAW264.7 macrophages. CONCLUSION: These findings could explain, at least in part, the successes reported in the use of the herb, Oldenlandia affinis in the traditional treatment of malaria fever Makerere Medical School 2017-09 /pmc/articles/PMC5656185/ /pubmed/29085411 http://dx.doi.org/10.4314/ahs.v17i3.26 Text en Copyright © Makerere Medical School, Uganda 2017 @ 2017 Nworu et al; licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Nworu, Chukwuemeka Sylvester
Ejikeme, Tochukwu Ifenyinwa
Ezike, Adaobi Chioma
Ndu, Okechukwu
Akunne, Theophine Chinwuba
Onyeto, Collins Azubuike
Okpalanduka, Paul
Akah, Peter Achunike
Anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of Oldenlandia affinis (R. & S.) D.C. (Rubiaceae)
title Anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of Oldenlandia affinis (R. & S.) D.C. (Rubiaceae)
title_full Anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of Oldenlandia affinis (R. & S.) D.C. (Rubiaceae)
title_fullStr Anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of Oldenlandia affinis (R. & S.) D.C. (Rubiaceae)
title_full_unstemmed Anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of Oldenlandia affinis (R. & S.) D.C. (Rubiaceae)
title_short Anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of Oldenlandia affinis (R. & S.) D.C. (Rubiaceae)
title_sort anti-plasmodial and anti-inflammatory activities of cyclotide-rich extract and fraction of oldenlandia affinis (r. & s.) d.c. (rubiaceae)
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656185/
https://www.ncbi.nlm.nih.gov/pubmed/29085411
http://dx.doi.org/10.4314/ahs.v17i3.26
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