Cargando…

Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa

BACKGROUND: The emergence of drug-resistant strains of Mycobacterium tuberculosis has become a global public health problem. Polyherbal medicines offer great hope for developing alternative drugs for the treatment of tuberculosis. OBJECTIVE: To evaluate the anti-tubercular activity of polyherbal med...

Descripción completa

Detalles Bibliográficos
Autores principales: Famewo, Elizabeth B, Clarke, Anna M, Wiid, Ian, Ngwane, Andile, van Helden, Paul, Afolayan, Anthony J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Makerere Medical School 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656206/
https://www.ncbi.nlm.nih.gov/pubmed/29085406
http://dx.doi.org/10.4314/ahs.v17i3.21
_version_ 1783273673276260352
author Famewo, Elizabeth B
Clarke, Anna M
Wiid, Ian
Ngwane, Andile
van Helden, Paul
Afolayan, Anthony J
author_facet Famewo, Elizabeth B
Clarke, Anna M
Wiid, Ian
Ngwane, Andile
van Helden, Paul
Afolayan, Anthony J
author_sort Famewo, Elizabeth B
collection PubMed
description BACKGROUND: The emergence of drug-resistant strains of Mycobacterium tuberculosis has become a global public health problem. Polyherbal medicines offer great hope for developing alternative drugs for the treatment of tuberculosis. OBJECTIVE: To evaluate the anti-tubercular activity of polyherbal medicines used for the treatment of tuberculosis. METHODS: The remedies were screened against Mycobacterium tuberculosis H37Rv using Middlebrook 7H9 media and MGIT BACTEC 960 system. They were liquid preparations from King Williams Town site A (KWTa), King Williams Town site B (KWTb), King Williams Town site C (KWTc), Hogsback first site (HBfs), Hogsback second site (HBss), Hogsback third site (HBts), East London (EL), Alice (AL) and Fort Beaufort (FB). RESULTS: The susceptibility testing revealed that all the remedies contain anti-tubercular activity with KWTa, KWTb, KWTc, HBfs, HBts, AL and FB exhibiting more activity at a concentration below 25 µl/ml. Furthermore, MIC values exhibited inhibitory activity with the most active remedies from KWTa, HBfs and HBts at 1.562 µg/ml. However, isoniazid showed more inhibitory activity against M. tuberculosis at 0.05 µg/ml when compare to the polyherbal remedies. CONCLUSION: This study has indicated that these remedies could be potential sources of new anti-mycobacterial agents against M. tuberculosis. However, the activity of these preparations and their active principles still require in vivo study in order to assess their future as new anti-tuberculosis agents.
format Online
Article
Text
id pubmed-5656206
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Makerere Medical School
record_format MEDLINE/PubMed
spelling pubmed-56562062017-10-30 Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa Famewo, Elizabeth B Clarke, Anna M Wiid, Ian Ngwane, Andile van Helden, Paul Afolayan, Anthony J Afr Health Sci Articles BACKGROUND: The emergence of drug-resistant strains of Mycobacterium tuberculosis has become a global public health problem. Polyherbal medicines offer great hope for developing alternative drugs for the treatment of tuberculosis. OBJECTIVE: To evaluate the anti-tubercular activity of polyherbal medicines used for the treatment of tuberculosis. METHODS: The remedies were screened against Mycobacterium tuberculosis H37Rv using Middlebrook 7H9 media and MGIT BACTEC 960 system. They were liquid preparations from King Williams Town site A (KWTa), King Williams Town site B (KWTb), King Williams Town site C (KWTc), Hogsback first site (HBfs), Hogsback second site (HBss), Hogsback third site (HBts), East London (EL), Alice (AL) and Fort Beaufort (FB). RESULTS: The susceptibility testing revealed that all the remedies contain anti-tubercular activity with KWTa, KWTb, KWTc, HBfs, HBts, AL and FB exhibiting more activity at a concentration below 25 µl/ml. Furthermore, MIC values exhibited inhibitory activity with the most active remedies from KWTa, HBfs and HBts at 1.562 µg/ml. However, isoniazid showed more inhibitory activity against M. tuberculosis at 0.05 µg/ml when compare to the polyherbal remedies. CONCLUSION: This study has indicated that these remedies could be potential sources of new anti-mycobacterial agents against M. tuberculosis. However, the activity of these preparations and their active principles still require in vivo study in order to assess their future as new anti-tuberculosis agents. Makerere Medical School 2017-09 /pmc/articles/PMC5656206/ /pubmed/29085406 http://dx.doi.org/10.4314/ahs.v17i3.21 Text en Copyright © Makerere Medical School, Uganda 2017 @ 2017 Famewo et al; licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Famewo, Elizabeth B
Clarke, Anna M
Wiid, Ian
Ngwane, Andile
van Helden, Paul
Afolayan, Anthony J
Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa
title Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa
title_full Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa
title_fullStr Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa
title_full_unstemmed Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa
title_short Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa
title_sort anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in eastern cape, south africa
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656206/
https://www.ncbi.nlm.nih.gov/pubmed/29085406
http://dx.doi.org/10.4314/ahs.v17i3.21
work_keys_str_mv AT famewoelizabethb antimycobacteriumtuberculosisactivityofpolyherbalmedicinesusedforthetreatmentoftuberculosisineasterncapesouthafrica
AT clarkeannam antimycobacteriumtuberculosisactivityofpolyherbalmedicinesusedforthetreatmentoftuberculosisineasterncapesouthafrica
AT wiidian antimycobacteriumtuberculosisactivityofpolyherbalmedicinesusedforthetreatmentoftuberculosisineasterncapesouthafrica
AT ngwaneandile antimycobacteriumtuberculosisactivityofpolyherbalmedicinesusedforthetreatmentoftuberculosisineasterncapesouthafrica
AT vanheldenpaul antimycobacteriumtuberculosisactivityofpolyherbalmedicinesusedforthetreatmentoftuberculosisineasterncapesouthafrica
AT afolayananthonyj antimycobacteriumtuberculosisactivityofpolyherbalmedicinesusedforthetreatmentoftuberculosisineasterncapesouthafrica