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A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech

Studies of neuronal network emergence during sensory processing and motor control are greatly facilitated by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the abili...

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Detalles Bibliográficos
Autores principales: Tomina, Yusuke, Wagenaar, Daniel A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656430/
https://www.ncbi.nlm.nih.gov/pubmed/28944754
http://dx.doi.org/10.7554/eLife.29839
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author Tomina, Yusuke
Wagenaar, Daniel A
author_facet Tomina, Yusuke
Wagenaar, Daniel A
author_sort Tomina, Yusuke
collection PubMed
description Studies of neuronal network emergence during sensory processing and motor control are greatly facilitated by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging, we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes.
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spelling pubmed-56564302017-10-26 A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech Tomina, Yusuke Wagenaar, Daniel A eLife Neuroscience Studies of neuronal network emergence during sensory processing and motor control are greatly facilitated by technologies that allow us to simultaneously record the membrane potential dynamics of a large population of neurons in single cell resolution. To achieve whole-brain recording with the ability to detect both small synaptic potentials and action potentials, we developed a voltage-sensitive dye (VSD) imaging technique based on a double-sided microscope that can image two sides of a nervous system simultaneously. We applied this system to the segmental ganglia of the medicinal leech. Double-sided VSD imaging enabled simultaneous recording of membrane potential events from almost all of the identifiable neurons. Using data obtained from double-sided VSD imaging, we analyzed neuronal dynamics in both sensory processing and generation of behavior and constructed functional maps for identification of neurons contributing to these processes. eLife Sciences Publications, Ltd 2017-09-25 /pmc/articles/PMC5656430/ /pubmed/28944754 http://dx.doi.org/10.7554/eLife.29839 Text en © 2017, Tomina et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Tomina, Yusuke
Wagenaar, Daniel A
A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
title A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
title_full A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
title_fullStr A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
title_full_unstemmed A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
title_short A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
title_sort double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656430/
https://www.ncbi.nlm.nih.gov/pubmed/28944754
http://dx.doi.org/10.7554/eLife.29839
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