Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment

Although long noncoding RNAs (lncRNAs) have been emerging as critical regulators in various tissues and biological processes, little is known about their expression and regulation during the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in inflammatory microenvironment. In t...

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Autores principales: Zhang, Qingbin, Chen, Li, Cui, Shiman, Li, Yan, Zhao, Qi, Cao, Wei, Lai, Shixiang, Yin, Sanjun, Zuo, Zhixiang, Ren, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656573/
https://www.ncbi.nlm.nih.gov/pubmed/29070806
http://dx.doi.org/10.1038/s41598-017-14451-4
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author Zhang, Qingbin
Chen, Li
Cui, Shiman
Li, Yan
Zhao, Qi
Cao, Wei
Lai, Shixiang
Yin, Sanjun
Zuo, Zhixiang
Ren, Jian
author_facet Zhang, Qingbin
Chen, Li
Cui, Shiman
Li, Yan
Zhao, Qi
Cao, Wei
Lai, Shixiang
Yin, Sanjun
Zuo, Zhixiang
Ren, Jian
author_sort Zhang, Qingbin
collection PubMed
description Although long noncoding RNAs (lncRNAs) have been emerging as critical regulators in various tissues and biological processes, little is known about their expression and regulation during the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in inflammatory microenvironment. In this study, we have identified 63 lncRNAs that are not annotated in previous database. These novel lncRNAs were not randomly located in the genome but preferentially located near protein-coding genes related to particular functions and diseases, such as stem cell maintenance and differentiation, development disorders and inflammatory diseases. Moreover, we have identified 650 differentially expressed lncRNAs among different subsets of PDLSCs. Pathway enrichment analysis for neighboring protein-coding genes of these differentially expressed lncRNAs revealed stem cell differentiation related functions. Many of these differentially expressed lncRNAs function as competing endogenous RNAs that regulate protein-coding transcripts through competing shared miRNAs.
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spelling pubmed-56565732017-10-31 Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment Zhang, Qingbin Chen, Li Cui, Shiman Li, Yan Zhao, Qi Cao, Wei Lai, Shixiang Yin, Sanjun Zuo, Zhixiang Ren, Jian Sci Rep Article Although long noncoding RNAs (lncRNAs) have been emerging as critical regulators in various tissues and biological processes, little is known about their expression and regulation during the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in inflammatory microenvironment. In this study, we have identified 63 lncRNAs that are not annotated in previous database. These novel lncRNAs were not randomly located in the genome but preferentially located near protein-coding genes related to particular functions and diseases, such as stem cell maintenance and differentiation, development disorders and inflammatory diseases. Moreover, we have identified 650 differentially expressed lncRNAs among different subsets of PDLSCs. Pathway enrichment analysis for neighboring protein-coding genes of these differentially expressed lncRNAs revealed stem cell differentiation related functions. Many of these differentially expressed lncRNAs function as competing endogenous RNAs that regulate protein-coding transcripts through competing shared miRNAs. Nature Publishing Group UK 2017-10-25 /pmc/articles/PMC5656573/ /pubmed/29070806 http://dx.doi.org/10.1038/s41598-017-14451-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Qingbin
Chen, Li
Cui, Shiman
Li, Yan
Zhao, Qi
Cao, Wei
Lai, Shixiang
Yin, Sanjun
Zuo, Zhixiang
Ren, Jian
Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment
title Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment
title_full Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment
title_fullStr Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment
title_full_unstemmed Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment
title_short Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment
title_sort expression and regulation of long noncoding rnas during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656573/
https://www.ncbi.nlm.nih.gov/pubmed/29070806
http://dx.doi.org/10.1038/s41598-017-14451-4
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