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Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes

Melanopsin (OPN4) is a photo-pigment found in a small subset of intrinsically photosensitive ganglion cells (ipRGCs) of the mammalian retina. These cells play a role in synchronizing the central circadian pacemaker to the astronomical day by conveying information about ambient light to the hypothala...

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Autores principales: Moraes, Maria Nathália, de Assis, Leonardo Vinícius Monteiro, Magalhães-Marques, Keila Karoline, Poletini, Maristela Oliveira, de Lima, Leonardo Henrique Ribeiro Graciani, Castrucci, Ana Maria de Lauro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656685/
https://www.ncbi.nlm.nih.gov/pubmed/29070825
http://dx.doi.org/10.1038/s41598-017-13939-3
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author Moraes, Maria Nathália
de Assis, Leonardo Vinícius Monteiro
Magalhães-Marques, Keila Karoline
Poletini, Maristela Oliveira
de Lima, Leonardo Henrique Ribeiro Graciani
Castrucci, Ana Maria de Lauro
author_facet Moraes, Maria Nathália
de Assis, Leonardo Vinícius Monteiro
Magalhães-Marques, Keila Karoline
Poletini, Maristela Oliveira
de Lima, Leonardo Henrique Ribeiro Graciani
Castrucci, Ana Maria de Lauro
author_sort Moraes, Maria Nathália
collection PubMed
description Melanopsin (OPN4) is a photo-pigment found in a small subset of intrinsically photosensitive ganglion cells (ipRGCs) of the mammalian retina. These cells play a role in synchronizing the central circadian pacemaker to the astronomical day by conveying information about ambient light to the hypothalamic suprachiasmatic nucleus, the site of the master clock. We evaluated the effect of a heat stimulus (39.5 °C) on clock gene (Per1 and Bmal1) expression in cultured murine Melan-a melanocytes synchronized by medium changes, and in B16-F10 melanoma cells, in the presence of the selective OPN4 antagonist AA92593, or after OPN4 knockdown by small interfering RNA (siRNA). In addition, we evaluated the effects of heat shock on the localization of melanopsin by immunocytochemistry. In both cell lines melanopsin was found in a region capping the nucleus and heat shock did not affect its location. The heat-induced increase of Per1 expression was inhibited when melanopsin was pharmacologically blocked by AA92593 as well as when its protein expression was suppressed by siRNA in both Melan-a and B16-F10 cells. These data strongly suggest that melanopsin is required for thermo-reception, acting as a thermo-opsin that ultimately feeds the local circadian clock in mouse melanocytes and melanoma cells.
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spelling pubmed-56566852017-10-31 Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes Moraes, Maria Nathália de Assis, Leonardo Vinícius Monteiro Magalhães-Marques, Keila Karoline Poletini, Maristela Oliveira de Lima, Leonardo Henrique Ribeiro Graciani Castrucci, Ana Maria de Lauro Sci Rep Article Melanopsin (OPN4) is a photo-pigment found in a small subset of intrinsically photosensitive ganglion cells (ipRGCs) of the mammalian retina. These cells play a role in synchronizing the central circadian pacemaker to the astronomical day by conveying information about ambient light to the hypothalamic suprachiasmatic nucleus, the site of the master clock. We evaluated the effect of a heat stimulus (39.5 °C) on clock gene (Per1 and Bmal1) expression in cultured murine Melan-a melanocytes synchronized by medium changes, and in B16-F10 melanoma cells, in the presence of the selective OPN4 antagonist AA92593, or after OPN4 knockdown by small interfering RNA (siRNA). In addition, we evaluated the effects of heat shock on the localization of melanopsin by immunocytochemistry. In both cell lines melanopsin was found in a region capping the nucleus and heat shock did not affect its location. The heat-induced increase of Per1 expression was inhibited when melanopsin was pharmacologically blocked by AA92593 as well as when its protein expression was suppressed by siRNA in both Melan-a and B16-F10 cells. These data strongly suggest that melanopsin is required for thermo-reception, acting as a thermo-opsin that ultimately feeds the local circadian clock in mouse melanocytes and melanoma cells. Nature Publishing Group UK 2017-10-25 /pmc/articles/PMC5656685/ /pubmed/29070825 http://dx.doi.org/10.1038/s41598-017-13939-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Moraes, Maria Nathália
de Assis, Leonardo Vinícius Monteiro
Magalhães-Marques, Keila Karoline
Poletini, Maristela Oliveira
de Lima, Leonardo Henrique Ribeiro Graciani
Castrucci, Ana Maria de Lauro
Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes
title Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes
title_full Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes
title_fullStr Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes
title_full_unstemmed Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes
title_short Melanopsin, a Canonical Light Receptor, Mediates Thermal Activation of Clock Genes
title_sort melanopsin, a canonical light receptor, mediates thermal activation of clock genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656685/
https://www.ncbi.nlm.nih.gov/pubmed/29070825
http://dx.doi.org/10.1038/s41598-017-13939-3
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