Low fraction of the 222K PrP variant in the protease-resistant moiety of PrP(res) in heterozygous scrapie positive goats

The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrP(res) formation in natural and experimental Q/K scrapie cases, we applied...

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Detalles Bibliográficos
Autores principales: Mazza, Maria, Guglielmetti, Chiara, Ingravalle, Francesco, Brusadore, Sonia, Langeveld, Jan P. M., Ekateriniadou, Loukia V., Andréoletti, Olivier, Casalone, Cristina, Acutis, Pier Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2017
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656779/
https://www.ncbi.nlm.nih.gov/pubmed/28691895
http://dx.doi.org/10.1099/jgv.0.000843
Descripción
Sumario:The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrP(res) formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrP(res) seems to be formed nearly totally by the 222Q variant provides evidence that the 222K PrP variant confers resistance to conversion to PrP(res) formation and reinforces the view that this mutation has a protective role against classical scrapie in goats.

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