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Clues for two-step virion infectivity factor regulation by core binding factor beta

Lentiviruses threaten human and animal health. Virion infectivity factor (Vif) is essential for the infectivity of most lentiviruses, except for the equine infectious anaemia virus (EIAV). Vif promotes viral infectivity by recruiting a Cullin-based E3 ligase to induce the degradation of a class of h...

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Autores principales: Ai, Youwei, Ma, Jianzhang, Wang, Xiaojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656798/
https://www.ncbi.nlm.nih.gov/pubmed/28516844
http://dx.doi.org/10.1099/jgv.0.000749
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author Ai, Youwei
Ma, Jianzhang
Wang, Xiaojun
author_facet Ai, Youwei
Ma, Jianzhang
Wang, Xiaojun
author_sort Ai, Youwei
collection PubMed
description Lentiviruses threaten human and animal health. Virion infectivity factor (Vif) is essential for the infectivity of most lentiviruses, except for the equine infectious anaemia virus (EIAV). Vif promotes viral infectivity by recruiting a Cullin-based E3 ligase to induce the degradation of a class of host restriction factors, named APOBEC3. Core binding factor beta (CBF-β) is necessary for several primate lentiviral Vif functions, including HIV-1 Vif. Although much progress has been made in understanding the contribution of CBF-β to Vif function, the precise mechanism has not yet been fully elucidated. In this study, we found that an interaction with CBF-β altered the oligomerization and subcellular distribution pattern and increased the stability of two primate lentiviral Vifs, HIV-1 Vif and Macaca simian immunodeficiency virus (SIVmac) Vif. Moreover, using a CBF-β loss-of-function mutant, we demonstrated that the interaction between CBF-β and Vif was not sufficient for Vif assistance; a region including F68 in CBF-β was also required for the stability and function of Vif. For the first time, this study separates the binding and regulating processes of CBF-β when it is promoting Vif function, which further extends our understanding of the biochemical regulation of Vif by CBF-β.
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spelling pubmed-56567982017-11-14 Clues for two-step virion infectivity factor regulation by core binding factor beta Ai, Youwei Ma, Jianzhang Wang, Xiaojun J Gen Virol Research Article Lentiviruses threaten human and animal health. Virion infectivity factor (Vif) is essential for the infectivity of most lentiviruses, except for the equine infectious anaemia virus (EIAV). Vif promotes viral infectivity by recruiting a Cullin-based E3 ligase to induce the degradation of a class of host restriction factors, named APOBEC3. Core binding factor beta (CBF-β) is necessary for several primate lentiviral Vif functions, including HIV-1 Vif. Although much progress has been made in understanding the contribution of CBF-β to Vif function, the precise mechanism has not yet been fully elucidated. In this study, we found that an interaction with CBF-β altered the oligomerization and subcellular distribution pattern and increased the stability of two primate lentiviral Vifs, HIV-1 Vif and Macaca simian immunodeficiency virus (SIVmac) Vif. Moreover, using a CBF-β loss-of-function mutant, we demonstrated that the interaction between CBF-β and Vif was not sufficient for Vif assistance; a region including F68 in CBF-β was also required for the stability and function of Vif. For the first time, this study separates the binding and regulating processes of CBF-β when it is promoting Vif function, which further extends our understanding of the biochemical regulation of Vif by CBF-β. Microbiology Society 2017-05 2017-05-18 /pmc/articles/PMC5656798/ /pubmed/28516844 http://dx.doi.org/10.1099/jgv.0.000749 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ai, Youwei
Ma, Jianzhang
Wang, Xiaojun
Clues for two-step virion infectivity factor regulation by core binding factor beta
title Clues for two-step virion infectivity factor regulation by core binding factor beta
title_full Clues for two-step virion infectivity factor regulation by core binding factor beta
title_fullStr Clues for two-step virion infectivity factor regulation by core binding factor beta
title_full_unstemmed Clues for two-step virion infectivity factor regulation by core binding factor beta
title_short Clues for two-step virion infectivity factor regulation by core binding factor beta
title_sort clues for two-step virion infectivity factor regulation by core binding factor beta
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656798/
https://www.ncbi.nlm.nih.gov/pubmed/28516844
http://dx.doi.org/10.1099/jgv.0.000749
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