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Effector‐mediated discovery of a novel resistance gene against Bremia lactucae in a nonhost lettuce species

Candidate effectors from lettuce downy mildew (Bremia lactucae) enable high‐throughput germplasm screening for the presence of resistance (R) genes. The nonhost species Lactuca saligna comprises a source of B. lactucae R genes that has hardly been exploited in lettuce breeding. Its cross‐compatibili...

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Autores principales: Giesbers, Anne K. J., Pelgrom, Alexandra J. E., Visser, Richard G. F., Niks, Rients E., Van den Ackerveken, Guido, Jeuken, Marieke J. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656935/
https://www.ncbi.nlm.nih.gov/pubmed/28833168
http://dx.doi.org/10.1111/nph.14741
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author Giesbers, Anne K. J.
Pelgrom, Alexandra J. E.
Visser, Richard G. F.
Niks, Rients E.
Van den Ackerveken, Guido
Jeuken, Marieke J. W.
author_facet Giesbers, Anne K. J.
Pelgrom, Alexandra J. E.
Visser, Richard G. F.
Niks, Rients E.
Van den Ackerveken, Guido
Jeuken, Marieke J. W.
author_sort Giesbers, Anne K. J.
collection PubMed
description Candidate effectors from lettuce downy mildew (Bremia lactucae) enable high‐throughput germplasm screening for the presence of resistance (R) genes. The nonhost species Lactuca saligna comprises a source of B. lactucae R genes that has hardly been exploited in lettuce breeding. Its cross‐compatibility with the host species L. sativa enables the study of inheritance of nonhost resistance (NHR). We performed transient expression of candidate RXLR effector genes from B. lactucae in a diverse Lactuca germplasm set. Responses to two candidate effectors (BLR31 and BLN08) were genetically mapped and tested for co‐segregation with disease resistance. BLN08 induced a hypersensitive response (HR) in 55% of the L. saligna accessions, but responsiveness did not co‐segregate with resistance to Bl:24. BLR31 triggered an HR in 5% of the L. saligna accessions, and revealed a novel R gene providing complete B. lactucae race Bl:24 resistance. Resistant hybrid plants that were BLR31 nonresponsive indicated other unlinked R genes and/or nonhost QTLs. We have identified a candidate avirulence effector of B. lactucae (BLR31) and its cognate R gene in L. saligna. Concurrently, our results suggest that R genes are not required for NHR of L. saligna.
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spelling pubmed-56569352017-11-01 Effector‐mediated discovery of a novel resistance gene against Bremia lactucae in a nonhost lettuce species Giesbers, Anne K. J. Pelgrom, Alexandra J. E. Visser, Richard G. F. Niks, Rients E. Van den Ackerveken, Guido Jeuken, Marieke J. W. New Phytol Research Candidate effectors from lettuce downy mildew (Bremia lactucae) enable high‐throughput germplasm screening for the presence of resistance (R) genes. The nonhost species Lactuca saligna comprises a source of B. lactucae R genes that has hardly been exploited in lettuce breeding. Its cross‐compatibility with the host species L. sativa enables the study of inheritance of nonhost resistance (NHR). We performed transient expression of candidate RXLR effector genes from B. lactucae in a diverse Lactuca germplasm set. Responses to two candidate effectors (BLR31 and BLN08) were genetically mapped and tested for co‐segregation with disease resistance. BLN08 induced a hypersensitive response (HR) in 55% of the L. saligna accessions, but responsiveness did not co‐segregate with resistance to Bl:24. BLR31 triggered an HR in 5% of the L. saligna accessions, and revealed a novel R gene providing complete B. lactucae race Bl:24 resistance. Resistant hybrid plants that were BLR31 nonresponsive indicated other unlinked R genes and/or nonhost QTLs. We have identified a candidate avirulence effector of B. lactucae (BLR31) and its cognate R gene in L. saligna. Concurrently, our results suggest that R genes are not required for NHR of L. saligna. John Wiley and Sons Inc. 2017-08-21 2017-11 /pmc/articles/PMC5656935/ /pubmed/28833168 http://dx.doi.org/10.1111/nph.14741 Text en © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Giesbers, Anne K. J.
Pelgrom, Alexandra J. E.
Visser, Richard G. F.
Niks, Rients E.
Van den Ackerveken, Guido
Jeuken, Marieke J. W.
Effector‐mediated discovery of a novel resistance gene against Bremia lactucae in a nonhost lettuce species
title Effector‐mediated discovery of a novel resistance gene against Bremia lactucae in a nonhost lettuce species
title_full Effector‐mediated discovery of a novel resistance gene against Bremia lactucae in a nonhost lettuce species
title_fullStr Effector‐mediated discovery of a novel resistance gene against Bremia lactucae in a nonhost lettuce species
title_full_unstemmed Effector‐mediated discovery of a novel resistance gene against Bremia lactucae in a nonhost lettuce species
title_short Effector‐mediated discovery of a novel resistance gene against Bremia lactucae in a nonhost lettuce species
title_sort effector‐mediated discovery of a novel resistance gene against bremia lactucae in a nonhost lettuce species
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656935/
https://www.ncbi.nlm.nih.gov/pubmed/28833168
http://dx.doi.org/10.1111/nph.14741
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