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Activation of gga-miR-155 by reticuloendotheliosis virus T strain and its contribution to transformation

The v-rel oncoprotein encoded by reticuloendotheliosis virus T strain (Rev-T) is a member of the rel/NF-κB family of transcription factors capable of transformation of primary chicken spleen and bone marrow cells. Rapid transformation of avian haematopoietic cells by v-rel occurs through a process o...

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Autores principales: Yao, Yongxiu, Vasoya, Deepali, Kgosana, Lydia, Smith, Lorraine P., Gao, Yulong, Wang, Xiaomei, Watson, Mick, Nair, Venugopal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5657028/
https://www.ncbi.nlm.nih.gov/pubmed/28113043
http://dx.doi.org/10.1099/jgv.0.000718
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author Yao, Yongxiu
Vasoya, Deepali
Kgosana, Lydia
Smith, Lorraine P.
Gao, Yulong
Wang, Xiaomei
Watson, Mick
Nair, Venugopal
author_facet Yao, Yongxiu
Vasoya, Deepali
Kgosana, Lydia
Smith, Lorraine P.
Gao, Yulong
Wang, Xiaomei
Watson, Mick
Nair, Venugopal
author_sort Yao, Yongxiu
collection PubMed
description The v-rel oncoprotein encoded by reticuloendotheliosis virus T strain (Rev-T) is a member of the rel/NF-κB family of transcription factors capable of transformation of primary chicken spleen and bone marrow cells. Rapid transformation of avian haematopoietic cells by v-rel occurs through a process of deregulation of multiple protein-encoding genes through its direct effect on their promoters. More recently, upregulation of oncogenic miR-155 and its precursor pre-miR-155 was demonstrated in both Rev-T-infected chicken embryo fibroblast cultures and Rev-T-induced B-cell lymphomas. Through electrophoresis mobility shift assay and reporter analysis on the gga-miR-155 promoter, we showed that the v-rel-induced miR-155 overexpression occurred by the direct binding to one of the putative NF-κB binding sites. Using the v-rel-induced transformation model on chicken embryonic splenocyte cultures, we could demonstrate a dynamic increase in miR-155 levels during the transformation. Transcriptome profiles of lymphoid cells transformed by v-rel showed upregulation of miR-155 accompanied by downregulation of a number of putative miR-155 targets such as Pu.1 and CEBPβ. We also showed that v-rel could rescue the suppression of miR-155 expression observed in Marek’s disease virus (MDV)-transformed cell lines, where its functional viral homologue MDV-miR-M4 is overexpressed. Demonstration of gene expression changes affecting major molecular pathways, including organismal injury and cancer in avian macrophages transfected with synthetic mature miR-155, underlines its potential direct role in transformation. Our study suggests that v-rel-induced transformation involves a complex set of events mediated by the direct activation of NF-κB targets, together with inhibitory effects on microRNA targets.
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spelling pubmed-56570282017-11-14 Activation of gga-miR-155 by reticuloendotheliosis virus T strain and its contribution to transformation Yao, Yongxiu Vasoya, Deepali Kgosana, Lydia Smith, Lorraine P. Gao, Yulong Wang, Xiaomei Watson, Mick Nair, Venugopal J Gen Virol Research Article The v-rel oncoprotein encoded by reticuloendotheliosis virus T strain (Rev-T) is a member of the rel/NF-κB family of transcription factors capable of transformation of primary chicken spleen and bone marrow cells. Rapid transformation of avian haematopoietic cells by v-rel occurs through a process of deregulation of multiple protein-encoding genes through its direct effect on their promoters. More recently, upregulation of oncogenic miR-155 and its precursor pre-miR-155 was demonstrated in both Rev-T-infected chicken embryo fibroblast cultures and Rev-T-induced B-cell lymphomas. Through electrophoresis mobility shift assay and reporter analysis on the gga-miR-155 promoter, we showed that the v-rel-induced miR-155 overexpression occurred by the direct binding to one of the putative NF-κB binding sites. Using the v-rel-induced transformation model on chicken embryonic splenocyte cultures, we could demonstrate a dynamic increase in miR-155 levels during the transformation. Transcriptome profiles of lymphoid cells transformed by v-rel showed upregulation of miR-155 accompanied by downregulation of a number of putative miR-155 targets such as Pu.1 and CEBPβ. We also showed that v-rel could rescue the suppression of miR-155 expression observed in Marek’s disease virus (MDV)-transformed cell lines, where its functional viral homologue MDV-miR-M4 is overexpressed. Demonstration of gene expression changes affecting major molecular pathways, including organismal injury and cancer in avian macrophages transfected with synthetic mature miR-155, underlines its potential direct role in transformation. Our study suggests that v-rel-induced transformation involves a complex set of events mediated by the direct activation of NF-κB targets, together with inhibitory effects on microRNA targets. Microbiology Society 2017-04 2017-04-28 /pmc/articles/PMC5657028/ /pubmed/28113043 http://dx.doi.org/10.1099/jgv.0.000718 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yao, Yongxiu
Vasoya, Deepali
Kgosana, Lydia
Smith, Lorraine P.
Gao, Yulong
Wang, Xiaomei
Watson, Mick
Nair, Venugopal
Activation of gga-miR-155 by reticuloendotheliosis virus T strain and its contribution to transformation
title Activation of gga-miR-155 by reticuloendotheliosis virus T strain and its contribution to transformation
title_full Activation of gga-miR-155 by reticuloendotheliosis virus T strain and its contribution to transformation
title_fullStr Activation of gga-miR-155 by reticuloendotheliosis virus T strain and its contribution to transformation
title_full_unstemmed Activation of gga-miR-155 by reticuloendotheliosis virus T strain and its contribution to transformation
title_short Activation of gga-miR-155 by reticuloendotheliosis virus T strain and its contribution to transformation
title_sort activation of gga-mir-155 by reticuloendotheliosis virus t strain and its contribution to transformation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5657028/
https://www.ncbi.nlm.nih.gov/pubmed/28113043
http://dx.doi.org/10.1099/jgv.0.000718
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